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http://dx.doi.org/10.5536/KJPS.2014.42.1.77

Construction of Genetic Linkage Map using Microsatellite and SNP Markers in Korean Native Chicken  

Seo, Dong Won (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Park, Hee Bok (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Choi, Nu Ri (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Jin, Shil (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Yoo, Chae Kyoung (Institute of Agriculture and Life Science, Gyeongsang National University)
Sultana, Hasina (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Heo, Kang Nyeong (Poultry Science Division, National Institute of Animal Science, RDA)
Jo, Cheorun (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University)
Lee, Jun Heon (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Poultry Science / v.42, no.1, 2015 , pp. 77-86 More about this Journal
Abstract
Chicken is one of the major livestock, especially for supplying proteins to human. The chicken genome size is approximately one-third compared with that of the human genome and regarded as a valuable model animal for genetics and development biology. In this study, we constructed the genetic linkage map for Korean native chicken (KNC) using 131 microsatellite (MS) and 8 single nucleotide polymorphism (SNP) markers. As a result, the total map length was calculated as 2729.4 cM and the average genetic distance between markers was 19.64 cM. The marker orders and genetic distances were well matched with the consensus linkage map except for the physical order of ADL0278 and MCW0351 in GGA8. In addition, the recombination rates in marcrochromosomes were 3.7 times higher than that of microchromosomes. The average numbers of alleles, expected heterozygosity (Hexp) and polymorphic information content (PIC) values were calculated as 5.5, 0.63 and 0.58, respectively. These results will give useful information for the understanding of genetic structure and QTL studies in KNC.
Keywords
Korean native chicken; linkage map; microsatellite; single nucleotide polymorphism(SNP);
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Times Cited By KSCI : 6  (Citation Analysis)
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