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http://dx.doi.org/10.5536/KJPS.2013.40.3.207

Comparative Study on the Viability of Frozen-thawed Primordial Germ Cells using Vitrification in Chicken Breed  

Kim, Hyun (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Kim, Dong Hun (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Han, Jae Yong (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Choi, Sung Bok (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Ko, Yeoung Gyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Do, Yoon Jung (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Seong, Hwan Hoo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Kim, Sung Woo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Publication Information
Korean Journal of Poultry Science / v.40, no.3, 2013 , pp. 207-216 More about this Journal
Abstract
This study was conducted to establish the method for preserving PGCs that enables long-term storage in liquid nitrogen for developmental engineering or preservation of species. The purpose of this study is to clarify the effects of freeze-thaw treatment on viability of PGCs in chickens. PGCs were collected separately from a germinal gonad of an early embryo of 5.5~6 day (stage 28) of Isa brown, Korean Oge (KO), White Leghorn and Commercial breeds. PGCs separated from a germinal gonad of an early embryo of 5.5~6 day (stage 28) are suspended in a freezing medium containing a freezing and protecting agents (e.g. dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol). The PGCs were then purified using magnetic activated cell sorting (MACS) method. The viability of PGCs after thawing was $87.4{\pm}0.4%$ and $89.4{\pm}0.2%$ with the 10% EG treatments with no significant difference between the Isa brown and Commercial breeds. The viability of PGCs after freeze- thawing was significantly higher for Isa brown ($87.4{\pm}0.4%$) and Commercial breeds ($89.4{\pm}0.2%$) than Korean Oge (KO) ($77.6{\pm}1.1%$) and White Leghorn ($76.2{\pm}0.9%$)(p<0.05) using 10% EG cryoprotectant. This study established a method for pre- serving chicken PGCs that enables systematic storage and labeling of cryopreserved PGCs in liquid ($LN_2$) at agermplasm repository and ease of entry into a data base. In the future, the importance for this new technology is that poultry lines can be conserved while work is being conducted on improving the production of germline chimeras.
Keywords
primordial germ cells (PGCs); vitrification; cryoprotective agents; viability; chicken breed;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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