Browse > Article
http://dx.doi.org/10.5536/KJPS.2012.39.4.273

Production of Immunospecific Egg Yolk Antibody with Recombinant Staphylococcal Enterotoxin B (SEB) Protein  

Lee, Seong (Department of Animal Resources and Science, Dankook University)
Lee, Sang-Rae (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jung, Kyung Min (Department of Animal Resources and Science, Dankook University)
Kim, Jung Woo (Department of Animal Resources and Science, Dankook University)
Publication Information
Korean Journal of Poultry Science / v.39, no.4, 2012 , pp. 273-278 More about this Journal
Abstract
Staphylococcal enterotoxin B (SEB), which is a bacterial superantigen produced by Staphylococcus aureus, is associated with serious diseases, including food poisoning and atopic dermatitis. This study was performed to produce about 30 kDa of recombinant SEB protein and to immunize in chickens to acquire the specific egg yolk antibody (IgY) against the recombinant SEB. Chickens were immunized with the recombinant SEB intramuscularly in the breast muscle by injection 3 times at intervals of two weeks. Serum- and egg yolk-antibody titers of hens against SEB were highest at 4 weeks after first immunization. In western blot, anti-recombinant SEB IgY was reacted immunospecifically against the recombinant SEB and commercialized SEB. These results suggested that the recombinant SEB antigen could be used as an immunogen to elicit antibody (IgY) against SEB and the anti-recombinant SEB IgY could neutralized staphylococcal enterotoxin B effectively.
Keywords
recombinant SEB; IgY; immune reaction;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Mathew AG, Rattanatabtimtong S, Nyachoti CM, Fang L 2009 Effects of in-feed egg yolk antibodies on Salmonella shedding, bacterial antibiotic resistance, and health of pigs. J Food Prot 72(2):267-273.   DOI
2 Shimizu M, Fitzsimmons RC, Nakai S 1988 Anti-E. coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. J Food Sic 53(5):1360-1366.   DOI
3 Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner EK, Goeke NM, Olson BJ, Klenk DC 1985 Measurement of protein using bicinchoninic acid. Anal Biochem 150:76-85.   DOI   ScienceOn
4 김정우 김도균 김철 2000 장관독성 대장균 K99(F5)의 섬모 항원에 대한 특이 난황항체의 생산. 한국동물자원학과학회지 42:371-378.
5 이희수 김종만 우승룡 정병열 조윤상 유한상 윤용덕 허원 문영식 오진식 2004 난황면역제를 이용한 개 주요 소화기 및 호흡기질병의 방제에 관한 연구 II. 난황면역제의 실험동물과 개에 있어서의 질병방제 효과. 대한수의학회 44(3):415-420.
6 Bar-Joseph Md, Malkinson M 1980 Hen egg yolk as a source of antiviral antibodies in the enzyme linked immunosorbent assay(ELISA): a comparison of two plant viruses. J Virol Methods 1:179.   DOI   ScienceOn
7 Dorte N, Pedersin LJ, Skov PS, Vejlsgaard GL, Poulsen LK, Jarlov JO, Karlsmak T, Nolte H 1997 IgE-binding components of staphylococcal enterotoxins in patients with atopic dermatitis. Ann Allergy Asthma Immunol 79:403-408.   DOI   ScienceOn
8 Knecht W, Kohler R, Minet M, Loffler M, 1996 Anti-peptide immunoglobulins from rabbit and chicken eggs recognize recombinant human dihydroorotate dehydrogenase and a 44-kDa protein from rat liver mitochondria. Eur J Biochem 236:609-613.   DOI   ScienceOn
9 Kovacs-Nolan J, Mine Y 2004 Passive immunization through avian egg antibodies. Food Biotechnology 18(1):39-62.   DOI   ScienceOn
10 LeClare RD, Hunt RE, Bavari S 2002 Protection against bacterial superantigen staphylococcal enterotoxin B by passive vaccination. Infect Immuni 70:2278-2281.   DOI
11 Lee SH, Lillehoj HS, Park DW, Jang SI, Morales A, García D, Lucio E, Larios R, Victoria G, Marrufo D, Lillehoj EP 2009 Protective effect of hyperimmune egg yolk IgY antibodies against Eimeria tenella and Eimeria maxima infections. Vet Parasitol 163(1-2):123-126.   DOI   ScienceOn
12 Li XY, Jin LJ, Uzonna JE, Li SY, Liu JJ, Li HQ, Lu YN, Zhen YH, Xu YP 2009 Chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY): In vivo evaluation in a pig model of enteric colibacillosis. Vet Immunol Immunopathol 129(1-2):132-136.   DOI   ScienceOn
13 Lin YT, Shau WY, Wang LF, Yang YH, Hwang YW, Tsai MJ, Tsao PN, Chiang BL 2000 Comparison of serum specific IgE antibodies to staphylococcal enterotoxins between atopic children with and without atopic dermatitis. Allergy 55:641-646.   DOI   ScienceOn