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http://dx.doi.org/10.5536/KJPS.2010.37.2.159

Virulence of Ornithobacterium rhinotracheale Isolates for Embryonated SPF Eggs and Broilers  

Kwon, Yong-Kuk (National Veterinary Research & Quarantine Service)
Jeon, Woo-Jin (National Veterinary Research & Quarantine Service)
Kang, Min-Soo (National Veterinary Research & Quarantine Service)
Oh, Jae-Young (National Veterinary Research & Quarantine Service)
An, Byung-Ki (National Veterinary Research & Quarantine Service)
Song, Eun-A (National Veterinary Research & Quarantine Service)
Kwon, Jun-Hun (National Veterinary Research & Quarantine Service)
Lee, Cheong-San (Chungbuk Livestock and Veterinary Research Institute)
Kim, Jae-Hong (College of Veterinary Medicine, Seoul National University)
Publication Information
Korean Journal of Poultry Science / v.37, no.2, 2010 , pp. 159-165 More about this Journal
Abstract
Field strains of Ornithobacterium rhinotracheale (OR) were tested on their virulence in specific pathogen free (SPF) embryonated chicken eggs and 3-week-old broilers. When infected with three different OR isolates (OR-161, OR-240 and OR-295) through yolk sac infection route, all strains appeared to be highly pathogenic with responsible mortality 66% and 100% within 12 days post infection (DPI). To test the virulence of OR in the commercial broilers, 3 week-old broilers were grouped depends on the inoculation route of OR isolate (OR-295) through five different infection routes; group 1 (IT: intratracheal), group 2 (IM: intramuscular), group 3 (IV: intravenous), group 4 (aerosol) and group 5 [Mixed: NDV (LaSota)+OR aerosol]. Within 5 to 7 days after inoculation, only broilers given NDV+OR were slightly depressed and coughing, and had mild facial redness. Grossly, foamy and yellow-white yogurt like exudate in the air sacs, predominantly in the abdominal air sacs was present. In histology, infiltration of the air sac epithelium and lamina propria by macrophage and polymorphonuclear granulocytes was seen with cell debris and inflammatory cells, correlated with the presence of OR antigen, as demonstrated by immunohistochemistry. Field strains of OR were able to induce high mortality in the embryonated chicken eggs, whereas broilers were less susceptible to OR infection. Interestingly, in the absence of NDV infection, the four groups of OR single infection only different route showed minimal and temporary microscopic air sac lesions. Thus, Newcastle disease virus (LaSota strain) showed triggering effects on the OR infection in chickens.
Keywords
Ornithobacterium rhinotracheale; broiler; embryonated egg; pathogenicity; newcastle disease;
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1 Sprenger SJ, Back A, Shaw DP, Nagaraja KV, Roepke DC, Halvorson DA 1998 Ornithobacterium rhinotracheale infection in turkeys: experimental reproduction of the disease. Avian Dis 42:154-161.   DOI   ScienceOn
2 Travers AF 1996 Concomitant Ornithobacterium rhinotracheale and Newcastle disease infection in broilers in South Africa. Avian Dis 40:488-490.   DOI   ScienceOn
3 Turan N, AK S 2002 Investigation of the presence of Ornithobacterium rhinotracheale in chickens in Turkey and determination of the seroprevalance of the infection using the enzyme-linked immunosorbent assay. Avian Dis 46:442-446.   DOI   ScienceOn
4 van Empel PCM, Hafez HM 1999 Ornithobacterium rhinotracheale: A review. Avian Path 28:217-227.   DOI   ScienceOn
5 van Empel P, van den Bosch H, Loeffen P, Stormand P 1997 Identification and serotyping of Ornithobacterium rhinotracheale. J Clin Microbiol 35:418-421.
6 van Empel P, Vrijenhoek M, Goovaerts D, van den Bosch H 1999 Immunohistochemical and serlogical investigation of experimental Ornithobacterium rhinotracheale in chickens. Avian Pathol 28:187-193.   DOI
7 van Emperl P, van den Bosch H, Goovaerts HD, Storm P 1996 Experimental infection in turkeys and chickens with Ornithobacterium rhinotracheale. Avian Dis 40:858-864.   DOI   ScienceOn
8 van Veen L, Hartman E, Fabri T 2001 In vitro antibiotic sensitivity of strains of Ornithobacterium rhinotracheale isolated in the Netherlands between 1996 and 1999. Vet Rec 149: 611-613.   DOI
9 Varga J, Fodor L, Makraiand L 2001 Characterisation of some Ornithobacterium rhinotracheale strains and examination of their transmission via eggs. Acta Vet Hungarica 49:125-130.   DOI   ScienceOn
10 권용국 전우진 김재홍 2003 닭에서 Ornithobacteriusm rhinotracheale 감염증에 대한 연구. 대한수의학회지 43:121-127.
11 De Rosa M, Droual R, Chin R, Shivaprasad H 1997 Interaction of Ornithobacterium rhinotracheale and Bordetella avium in turkey poults. In proceedings of the 46th Western Poultry Disease Conference, Sacramento pp 52-53.
12 Devriese LA, De Herdt P, Haesebrouck F 2001 Antibiotic sensitivity and resistance in Ornithobacterium rhinotracheale strains from Belgian broiler chickens. Avian Path 30:197-200.   DOI   ScienceOn
13 Devriese LA, Hommez J, Vandamme P, Kerster K, Haesebrouck F 1995 In vitro antibiotic sensitivity of Ornithobacterium rhinotracheale strains from poultry and wild birds. Vet Rec 137:435-436.   DOI
14 Hafez HM, Beyer W 1997 Preliminary investigation on Ornithobacterium rhinotracheale isolates using PCR-fingerprints. In proceedings of 11th International Congress of the World Veterinary Poultry Association. Budapest p 51.
15 Heeder CJ, Lopes VC, Nagaraja KV, Shaw DP, Halvorson DA 2001 Seroprevalence of Ornithobacterium rhinotracheale infection in commercial laying hens in the north central region of the United States. Avian Dis 45:1064-1067.   DOI   ScienceOn
16 Hinz KH, Blome C, Ryll M 1994 Acute exudative pneumonia and airsacculitis associated with Ornithobacterium rhinotracheale in turkeys. Vet Rec 135:233-234.   DOI
17 Ak S, Turan N 2001 Antimicrobial susceptibility of Ornithobacterium rhinotracheale isolated from broiler chickens in Turkey. Veterinarski Arhiv 71:121-127.
18 Joubert P, Higgins R, Laperle A, Mikaelian I, Venne D, Silim A 1999 Isolation of Ornithobacterium rhinotracheale from Turkeys in Quebec, Canada. Avian Dis 43:622-626.   DOI   ScienceOn
19 Roepke DC, Back A, Shaw DP, Nagaraja KV, Sprenger SJ, Halvorson DA 1998 Isolation and identification of Ornithobacterium rhiontracheale from commercial turkey flocks in the upper midwest. Avian Dis 42: 219-221.   DOI   ScienceOn
20 Sakai E, Tokuyama Y, Nonaka F, Ohishi S, Ishikawa Y, Tanaka M, Taneno A 2000 Ornithobacterium rhinotracheale infection in Japan: Preliminary investigations. Vet Rec 146:502-503.   DOI
21 Back A, Halvorson D, Rajashekara G, Nagaraja KV 1998a Development of a serum plate agglutination test to detect antibodies to Ornithobacterium rhinotracheale. J Vet Diagn Invest 10:84-86.   DOI   ScienceOn
22 Back A, Rajashekara G, Jeremiah RB, Halvorson DA, Nagaraja KV 1998b Tissue distribution of Ornithobacterium rhinotracheale in experimentally infected turkey. Vet Rec 12: 627-632.
23 Chin RP, van Empel PCM, Hafez HM 2003 Ornithobacterium rhinotracheale infection, p.683-690. In Y. M. Saif (ed.), Diseases of Poultry, 11th ed. Ames, Iowa State University Press.