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http://dx.doi.org/10.5536/KJPS.2008.35.1.009

Rapid Detection and Discrimination of the Three Salmonella Serotypes, S. Pullorum, S. Gallinarum and S. Enteritidis by PCR-RFLP of ITS and fliC Genes  

Cha, Se-Yeoun (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Jang, Du-Hee (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kim, Sang-Min (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Park, Jong-Beom (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Jang, Hyung-Kwan (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Publication Information
Korean Journal of Poultry Science / v.35, no.1, 2008 , pp. 9-13 More about this Journal
Abstract
Salmonella enterica serotype gallinarum biovar Gallinarum or Pullorum and Salmonella enterica serotype Enteritidis are the most important diseases in poultry industry. Transitional diagnosis methods of these diseases such as direct isolation and identification by a biochemical test are time consuming with low specificity. In this study, we have focused on the suitable procedure for the rapid and accurate diagnosis of diseases derived from the three Salmonella strains. We initially confirmed Salmonella species by PCR using a specific ITSF/ITSR primer pair instead of biochemical test, and then the PCR-amplified phase 1 flagellin (fliC) using a specific fliCF/fliCR primer pair was digested with a restriction endonuclease, Bpm I and/or Bfa I, to discriminate among S. Pullorum, S. Gallinarum, and S. Enteritidis. We found that these methods could be applied to field isolates of the three Salmonella strains to detect and to discriminate rapidly for convenient diagnosis.
Keywords
Salmonella; diagnosis; rapid detection; strain differentiation;
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