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http://dx.doi.org/10.5536/KJPS.2007.34.2.085

Identification of Differentially Expressed Genes in Four Different Growing Stages in Korea Native Chicken Liver  

Lee, K.Y. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Yu, S.L. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Jung, K.C. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Jang, B.K. (National Livestock Research Institute, R.D.A.)
Choi, K.D. (School of Biotechnology, Hankyong National University)
Lee, J.H. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Publication Information
Korean Journal of Poultry Science / v.34, no.2, 2007 , pp. 85-90 More about this Journal
Abstract
The chicken liver has been involved in various biological functions including detoxification, glycogen storage and plasma protein synthesis. The aim of this study was to investigate differentially expressed genes in chicken liver in four different growing stages. Using 10 arbitrary Annealing Control Primers (ACPs), five differentially expressed genes have been identified. Based on the Basic Local Alignment Search Tool (BLAST) search results, three of them were matched with previously known genes, and the other two were matched with unknown EST sequence and a hypothetical protein, respectively. In order to confirm the expression results, quantitative real-time PCR was also performed. The high similarities between the expression data using arbitrary ACPs and quantitative real-time PCR indicate that the identified genes are the real differentially expressed genes in different growing stages. The genes identified in this study can be used as valuable biomarkers in chicken with further investigation of the functions.
Keywords
annealing control primers; chicken liver; differentially expressed genes; growing stages;
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