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Analysis of Telomere Length and Telomerase Activity of Tissues in Korean Native Chicken  

Jung G.S. (Department of Animal Science & Biotechnology, Jinju National University)
Cho E.J. (Department of Animal Science & Biotechnology, Jinju National University)
Choi D.S. (Department of Animal Science & Biotechnology, Jinju National University)
Lee M.J. (Department of Animal Science & Biotechnology, Jinju National University)
Park C. (Department of Animal Science & Biotechnology, Jinju National University)
Jeon I.S. (Division of Animal Biotechnology, National Livestock Research Institute)
Sohn S.H. (Department of Animal Science & Biotechnology, Jinju National University)
Publication Information
Korean Journal of Poultry Science / v.33, no.2, 2006 , pp. 97-103 More about this Journal
Abstract
Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Even though telomere length and telomerase activity have been studied extensively, very little is known to analyze the telomere dynamics in chicken cells. This study was carried out to analyze the telomere distribution and telomerase activity of Korean Native Chicken cells along with aging. The cells were collected from brain, heart, liver, kidney and germinal tissues during physiological stages. Telomere distribution was analyzed by Quantitative-Fluorescence in situ Hybridization (Q-FISH) techniques using the chicken telomeric DNA probe. Telomerase activity was performed by Telomeric Repeat Amplification Protocol (TRAP) assay. In results, the telomeres of chicken were found at the ends of all chromosomes with the interstitial telomeres on chromosomes 1, 2 and 3. The amount of telomeres on chicken cells was decreased along with aging in most tissues. Furthermore, the telomere quantity was significantly different among tissues. The relative amount of telomeres in proliferous cells such as testis cells had much more than those of liver, brain, heart, blood and kidney cells. The telomerase activity was down-regulated in cells of brain, heart and liver tissues. Whereas gonadal cells showed a constitutive activity of telomerase during all stage of life. In conclusions, the telomere quantity and telomerase activity in chicken are closely relate to cell proliferation and tissue specificity during developmental stages and aging. There is also closely correlated between the amounts of telomeric DNA and telomerase activity in chicken tissues.
Keywords
chicken tissues; telomere; telomerase; FISH; TRAP;
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