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A Study of the Retrovirus-Mediated Transgenic Chicken Production on Chicken Embryos  

Byun S. J. (National Livestock Research Institute, Division of Animal Biotechnology)
Park C. (National Livestock Research Institute, Division of Animal Biotechnology, Department of Animal Biotechnology, Jinju National University)
Kim S. W. (National Livestock Research Institute, Division of Animal Biotechnology)
Park J. K. (National Livestock Research Institute, Division of Animal Biotechnology)
Chang W. K. (National Livestock Research Institute, Division of Animal Biotechnology)
Yang B. S. (National Livestock Research Institute, Division of Animal Biotechnology)
Kim T. Y. (The Catholic University of Korea)
Sohn S. H. (Department of Animal Biotechnology, Jinju National University)
Kim S. H. (Department of Biology, Kyung Hee University)
Jeon I. S. (National Livestock Research Institute, Division of Animal Biotechnology)
Publication Information
Korean Journal of Poultry Science / v.32, no.4, 2005 , pp. 225-229 More about this Journal
Abstract
Microinjection of recombinant retrovirus beneath the blastoderm of non-incubated chicken embryo is now the most widespread method for generating transgenic chickens, but transgenesis rates are very low. So to improve this problem, we first introduced retrovirus vector carrying RSV-GFP gene to an one-cell embryo culture system. To investigate whether retrovirus could work on an one-cell chicken embryo, we microinjected the concentrated retrovirus stocks into the germinal disc of one cell or stage-X chicken embryos. Analysis of reporter gene expression on day 4 embryos showed that GFP expression was observed in the only stage-X chicken embryo but was not in the one-cell embryo group. These results suggest that retrovirus system is the most efficient method to generate transgenic chickens in the stage-X embryo.
Keywords
retrovirus; microinjection; GFP; chicken embryo;
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