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Effect of Dietary Betaine and Energy Levels on Liver Fats and Cholesterol in Laying Hens  

Park J. H. (Department of Animal Resources and Biotechnology, Chonbuk National University)
Park S. Y. (Department of Animal Resources and Biotechnology, Chonbuk National University)
Ryu K. S. (Department of Animal Resources and Biotechnology, Chonbuk National University)
Publication Information
Korean Journal of Poultry Science / v.32, no.3, 2005 , pp. 149-156 More about this Journal
Abstract
Two experiments were conducted to examine the effects of betaine intake on blood and yolk cholesterol, abdominal fat, liver fat, tissue triglyceride(TG) and liver HMG-CoA reductase In laying hens. In Expt. 1, a total of 72 ISA-brown laying hens were individually assigned into four treatments from 18 to 21 weeks old. Com-soybean meal based diet were fed with the addition of 0, 300, 600 and 1,200ppm. In Expt. 2, 72 ISA-brown laying hens were housed into individual cage to evaluate the effect of dietary betaine(0, 600ppm) and energy(ME, 2,800, 2,900kca/kg) from 70 to 74 weeks. Serum total cholesterol, LDL and HDL-cholesterol and TG concentration in blood of hens fed betaine tended to increase compared to those of the control, but were not significantly different. However, betaine supplementation showed a statistically significant decrease in yolk cholesterol(P<0.05). There was no significant difference in abdominal fat among the treatments. Liver fats and 7c of birds 130 betaine was decreased compared with control. Serum total cholesterol, LDL-cholesterol and triglyceride concentration were significantly inc.eased by ffeding a diet containing 600ppm betaine in Expt. 2(P<0.05), but were not influenced by the dietary energy levels. Yolk cholesterol, abdominal fat and HMG-CoA reductase activity were affected neither by dietary energy nor betaine level.
Keywords
betaine; energy; fat; cholesterol; laying hens;
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1 Simon J 1999 Choline, betaine and methionine interactions in chickens, pigs and fish including crustaceans. World's Poult Sci 55:353-374   DOI   ScienceOn
2 Yao Z, Vance DE 1989 Head group specificity in the requirement of phosphotidylcholine biosnthesis for very low density lipoprotein secretion from cultured hepatocytes. Journal of Biological Chemistry 264: 11373-11380
3 Wolford JH, Polin D 1975 Effect of inositol, lecithin, vitamins (B12 with choline and E), and iodinated casein on induced fatty liver-hemorrhagic syndrome in laying chickens. Poultry Sci 54:981-991   DOI   ScienceOn
4 Barak AJ, Beckenhauer HC, Tuma DJ 1994 S-adenosylmethionine generation and prevention of alcoholic fatty liver by betaine. Alcohol 11:501-503   DOI   ScienceOn
5 Beckenhauer HC, Barak AJ, Tuma DJ 1993 Relationship of administration dietary levels of betaine to hepatic S-adnosylmethionine and triglyceride. Hepatology 18:254
6 Cadogan DJ, Campbell RG, Harrison D, Edwards AC 1993 The effects of betaine on the growth performance and carcass characteristics of female pigs. In: Batterham, E.S. (Ed.), Manipulating Pig Production IV. Australasian Pig Science Assocation, Attwood Victoria, Australia, pp. 219
7 De Ridder JJ, Van Dam K 1975 Control of choline oxidation by rat-liver mitochondria. Biochim Biophys Acta 408: 112-122   DOI   ScienceOn
8 Fenton M, Sim JS 1991 Determination of egg yolk cholesterol content by on-column capillary gas chromatography. J Chromatography 540:323-329   DOI   ScienceOn
9 Folch J, Lees M, Sloane-Stanley GH 1957 A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chern 226:497-509
10 Hulcher FH, Oleson WH 1973 Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. J Lipid Res 14: 625-631
11 Klasing KC, Adler KL, Calvert CC, Remus JC 2001 Effect of dietary betaine on intestinal leukocyte numbers, osmolality, and morphology during an Eimeria acervulina challenge. J Anim Sci 79(Suppl 1):170. (Abstr.)
12 Matthews JO, Southern LL, Higbie AD, Persica MA. Bidner TD 2001 Effects of betaine on growth, carcass characteristics, pork quality, and plasma metabolites of finishing pigs. J Anim Sci 79:722-728
13 Schutte JB, De Jong J, Smink W, Pack M 1997 Replacement value of betaine for DL-methionine in male broiler chicks. Poultry Sci 76:321-325   DOI
14 Schexnailder R, Griffith M 1973 Liver fat and egg production of laying hens as influenced by choline and other nutrients. Poultry Sci 52:1188-1194   DOI   ScienceOn
15 Bocan TM, Mueller SB, Brown EQ, Lee P, Bocan MJ, Rea T, Pape ME 1998 HMG-CoA reductase and ACAT inhibitors act synergistically to lower plasma cholesterol and limit atherosclerotic lesion development in the cholesterol-fed rabbit. Atherosclerosis 139:21-30   DOI   ScienceOn
16 Hay JW, Yu WM, Ashraf T 1999 Pharmacoeconornics of lipidlowering agents for primary and secondary prevention of coronary artery disease. Pharmacoeconornics 15:47-74   DOI   ScienceOn
17 Saunderson CL, MacKinlay J 1990 Changes in body-weight, composition and hepatic enzyme activities in response to dietary methionine, betaine and choline levels in growing chicks. British Journal of Nutrition 63:339-349   DOI   ScienceOn
18 Matthews JO, Southern LL, Pontif JE, Higbie AD, Bidner TD 1998 Interactive effects of betaine, crude protein, and net energy in finishing pigs. J Anim Sci 76:2444-2455
19 SAS Instutute 1996 $SAS/STAT^{circledR}$ Guide Version 6.12. SAS Institute Inc. Cary NC
20 Cromwell GL, Lindemann MD, Randolph JR, Monegue HJ, Laurent KM, Parker GR 1999 Efficacy of betaine as a carcass modifier in finishing pigs fed normal and reduced energy diets. J Anim Sci 77(Suppl 1): 179 (Abstr)
21 Overland M, Rorvik KA, Skrede A 1999 Effect of trimethylamine oxide and betaine in swine diets on growth performance, carcass characteristics, nutrient digestibility, and sensory quality of pork. J Anim Sci 77:2143-2153   DOI
22 Mondola P, Santillo M, De Mercato R, Santangelo F 1992 The effect of L-carnitine on cholesterol metabolism in rat(Rattus bubalus) hepatocyte cells. Int J Biochem 24:1047-1050   DOI   ScienceOn
23 Pettey LA, Cromwell GL, Lindemann MD, Randolph JR, Mo-negue ill, Laurent KM, Parker R, Coffey RD 2001 Efficacy of betaine as a carcass modifier in finishing pigs fed normal and low protein diets supplemented with amino acids. J Anim Sci 79(Suppl 1):183 (Abstr)
24 Harper AE, Monson WJ, Benton DA, Elvehjem CA 1953 The influence of protein and certain amino acids, particularly threonine, on the disposition of fat in the liver of the rats. J Nutrition 50:383   DOI
25 Stryer L 1988 Biochemistry. 3rd Ed Freeman, New York pp.935
26 Steel RGD, Tome JH 1980 Principles and procedure of statistics. McGraw Hill New York
27 Daily JW, Sachan DS 1995 Choline supplementation alters carnitine homeostasis in humans and guinea pigs. J Nutr 125: 1938-1944
28 Sugiyama K, Akai H, Muramatsu K 1986 Effects of methionine and related compounds on plasma cholesterol level in rats fed a high cholesterol diet. J Nutr Sci Vitaminol 32:537-549   DOI
29 Femandez-Figares I, Wray-Cahen D, Steele NC, Campbell RG, Hall DD, Virtanen E 2002 Effect of dietary betaine on nutrient utilization and partitioning in the young growing feed-restricted pig. J Anim Sci 80:421-428
30 Casarin A, Forat M, Zabaras-Krick BJ 1997 Interrelationships between betaine (Betafin-BCR) and level of feed intake on the performance parameters and carcass characteristics of growing-finishing pigs. J Anim Sci 75(Suppl 1):75 (Abstr)
31 Ghoshal AK, Ahluwalia M, Farber E 1983 The rapid induction of liver cell death in rats fed a choline-deficient methioninelow diet. Am J Pathol 113:309-314
32 Kettunen H, Peuranen S, Tiihonen K 2001 Betaine aids in the osmoregulation of duodenal epithelium of broiler chicks, and affects the movement of water across the intestinal epithelium in vitro. Comp Biochem Physiol A 129:595-603   DOI   ScienceOn
33 Kidd MT, Ferket PR, Garlich JD 1997 Nutritional and osmoregulatory functions of betaine. World Poul Sci 53: 125139