Morphological Study on the Mast Cell of Proventriculus in Pheasant (Phasianus colchicus)

Mast cells have been studied extensively in various animals including rats and mice, whereas little is known the morphological data about pheasant mast cells. Here, morphological features of Korean pheasant mast cells are described in this study using light and electron microscopes. For light microscopy, mast cells had many metachromatic granules stained with toluidine blue in the cytoplasm. The fixation with $10\%$ neutral buffered formalin blocked staining of most mast cells but a modified Karnovsky solution proved to be a good fixative. In Korean pheasants, toluidine blue stained more mast cells than did alcian blue. For electron microscopy, the mast cells of the Korean pheasant were round, oval, spindle-like and irregular form and occasionally had a few short cytoplasmic processes. These cells had membrane-bounded granules and poorly developed organells. Some granules in the cytoplasm of the mast cells had bilayer membrane. Most granules were round shape and the membrane of several granules was concave or convex. The granules were composed of three parts, homogenous, particulate and reticular pattern.

꿩 전위의 비만세포에 관한 형태학적 연구

꿩 비만세포는 원형, 난원형, 방추형 또는 부정형이었고 세포질돌기를 가지고 있었다. 꿩 비만세포는 Karnovsky 용액으로 고정했을 때 가장 좋은 염색성을 얻었고, Toluidine blue로 염색을 했을 때 비만세포의 과립은 이염색성(meta-chromatic)을 나타내었다. 비만세포의 세포질에는 막으로 둘러싸인 과립과 발달이 미약한 세포소기관으로 채워져 있었다. 비만세포의 과립은 원형과 난원형이었고 일부 과립은 막이 함몰된 오목한 모양과 밖으로 돌출된 볼록한 모양이었다. 과립 속의 구조는 동질성, 입자형 또는 망상형이었다.