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http://dx.doi.org/10.5857/RCP.2014.3.4.79

Regulation of DNAzyme function by hypoxic irradiation that induces one-electron reduction of 2-oxoalkyl group on thymine base  

Kanezaki, Hiroshi (Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University)
Nishimoto, Sei-Ichi (Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University)
Tanabe, Kazuhito (Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University)
Publication Information
Rapid Communication in Photoscience / v.3, no.4, 2014 , pp. 79-80 More about this Journal
Abstract
We characterized the one-electron reduction of oligodeoxynucleotides with a 2-oxopropyl group on a thymine base ($d^{oxo}T$) and applied the reaction to the radiolytic activation of DNAzyme function. We designed a system in which the DNAzyme function of cleaving mRNA was suppressed by introduction of $d^{oxo}T$ into the strand of DNAzyme. Hypoxic X-irradiation led to recovery of the cleavage ability because the 2-oxopropyl group was removed to form unmodified DNAzyme. We characterized the DNAzyme function by monitoring the fluorescence change of fluorophore- and quencher-labeled target strands. We confirmed that the DNAzyme function could be regulated by hypoxic X-irradiation and the reaction of $d^{oxo}T$.
Keywords
X-irradiation; DNAzyme; Artificial oligonucleotides;
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