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http://dx.doi.org/10.7853/kjvs.2022.45.3.229

Monitoring for fluoroquinolones residues in raw meat in Sejong  

Jeong, Yoon-Kyung (Sejong Institute of Health and Environment)
Lee, Taeho (Sejong Institute of Health and Environment)
Lee, Jong Hoon (Sejong Institute of Health and Environment)
Kim, Mun-Bae (Sejong Institute of Health and Environment)
Publication Information
Korean Journal of Veterinary Service / v.45, no.3, 2022 , pp. 229-236 More about this Journal
Abstract
This study describes an analytical method based on LC-MS/MS for the quantitation of 5 fluoroquinolones (Enrofloxacin, Ciprofloxacin, Marbofloxacin, Norfloxacin and Danofloxacin) in meat, and was applied to 230 meat samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations (R2≥0.998) were obtained for all fluoroquinolones at 6 concentrations of 1~50 ㎍/kg. Satisfied recoveries of all fluoroquinolones were demonstrated in spiked meat at three levels from 10 to 50 ㎍/kg. The recoveries ranged between 75.8~99.2% in beef, 80.1~99.6% in pork and 72.2~99.8% in chicken, respectively. The limits of quantitation (LOQs) for fluoroquinolones ranged from 0.7 to 3.2 ㎍/kg. We also monitored fluoroquinolones residue in the sample (beef 107, pork 71, chicken 52) using LC-MS/MS. Residues of fluoroquinolones which exceeded maximum residue limits (MRL) were not exceed in any of the 230 samples.
Keywords
Fluoroquinolones; Residue; LC-MS/MS; Law meat; Sejong;
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