Method development for efficacy testing of veterinary disinfectants using bacteriophage MS2
![]() |
Rhee, Chae Hong
(Veterinary Drugs & Biologics Division, Department of Animal Disease Control & Quarantine, Animal and Plant Quarantine Agency)
Kim, Soohee (Veterinary Drugs & Biologics Division, Department of Animal Disease Control & Quarantine, Animal and Plant Quarantine Agency) Han, Bokhee (Veterinary Drugs & Biologics Division, Department of Animal Disease Control & Quarantine, Animal and Plant Quarantine Agency) Kim, Young-Wook (Veterinary Drugs & Biologics Division, Department of Animal Disease Control & Quarantine, Animal and Plant Quarantine Agency) Her, Moon (Veterinary Drugs & Biologics Division, Department of Animal Disease Control & Quarantine, Animal and Plant Quarantine Agency) Jeong, Wooseog (Veterinary Drugs & Biologics Division, Department of Animal Disease Control & Quarantine, Animal and Plant Quarantine Agency) |
1 | 농림축산검역본부. 2018. 소독제 효력시험지침. 농림축산검역본부 고시 제2018-16호. |
2 | 배경선, 신귀암. 2016. 하수 처리 과정의 염소 소독에 대한 여러 박테리오파지들의 저항성 평가; 물 재이용 과정의 안전성 관리를 위한 바이러스 지표미생물의 개발. 상하수도학회지 30: 285-291. DOI |
3 | Adcock NJ, Rice EW, Sivaganesan M, Brown JD, Stallknecht DE, Swayne DE. 2009. The use of bacteriophages of the family Cystoviridae as surrogates for H5N1 highly pathogenic avian influenza viruses in persistence and inactivation studies. J Environ Sci Health A Tox Hazard Subst Environ Eng 44:1362-1366. DOI |
4 | Aquino de Carvalho NA, Stachler EN, Cimabue N, Bibby K. 2017. Evaluation of Phi6 persistence and suitability as an enveloped virus surrogate. Environ Sci Technol 51: 8692-8700. DOI |
5 | Buckley D, Dharmasena M, Wang H, Huang J, Adams J, Pettigrew C, Fraser A, Jiang X. 2020, Efficacy of novel aqueous photo-chlorine dioxide against a human norovirus surrogate, bacteriophage MS2 and Clostridium difficile endospores, in suspension, on stainless steel and under greenhouse conditions. J Appl Microbiol 130: 1531-1545. |
6 | Cho M, Chung H, Choi W, Yoon J. 2005. Different inactivation behaviors of MS-2 phage and Escherichia coli in TiO2 photocatalytic disinfection. Appl Environ Microbiol 71: 270-275. DOI |
7 | Prodelalova J, Malenovska H, Moutelikova R, Titera D. 2017. Virucides in apiculture: persistence of surrogate enterovirus under simulated field conditions. Pest Manag Sci 73: 2544-2549. DOI |
8 | Turgeon N, Toulouse MJ, Martel B, Moineau S, Duchaine C. 2014. Comparison of five bacteriophages as models for viral aerosol studies. Appl Environ Microbiol 80: 4242-4250. DOI |
9 | Kampf G, Grotheer D, Steinmann J. 2005. Efficacy of three ethanol-based hand rubs against feline calicivirus, a surrogate virus for norovirus. J Hosp Infect 60: 144-149. DOI |
10 | Cormier J, Janes M. 2014. A double layer plaque assay using spread plate technique for enumeration of bacteriophage MS2. J Virol Methods 196: 86-92. DOI |
11 | US EPA. 2001. Method 1601: Male-specific (F+) and somatic coliphage in water by two-step enrichment procedure. U.S. Environmental Protection Agency, Washington, DC, EPA 821-R-01-030. https://www.epa.gov/sites/default/files/2015-12/documents/method_1601_2001.pdf. |
12 | Wyrzykowska-Ceradini B, Calfee M, Touati A, Wood J, Mickelsen R, Miller L, Colby M, Slone C, Gatchalian N, Pongur S, Aslett D. 2019. The use of bacteriophage MS2 for the development and application of a virucide decontamination test method for porous and heavily soiled surfaces. J Appl Microbiol 127: 1315-1326. DOI |
13 | Eggers M, Schwebke I, Suchomel M, Fotheringham V, Gebel J, Meyer B, Morace G, Roques C, Visa P, Steinhauer K. 2021. The European tiered approach for virucidal efficacy testing - rationale for rapidly selecting disinfectants against emerging and re-emerging viral diseases. Euro Surveill 26 pii=2000708. https://doi.org/10.2807/1560-7917.ES.2021.26.3.2000708. DOI |
14 | 서영석, 김애린, 조민. 2019. 전해 염소수/자외선 결합 시스템을 이용한 병원성 미생물의 불활성화 키네틱스 평가. 상하수도학회지 33: 379-388. DOI |
15 | US EPA. 2016. Effectiveness of spray-based decontamination methods for spores and viruses on heavily soiled surfaces. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-16/162. file:///C:/Users/LG/Downloads/EFFECTIVENESS+OF+SPRAYBASED+DECONTAMINATION.PDF. |
16 | Gallandat K, Lantagne D. 2017. Selection of a Biosafety Level 1 (BSL-1) surrogate to evaluate surface disinfection efficacy in Ebola outbreaks: Comparison of four bacteriophages. PLoS ONE 12: e0177943. https://doi.org/10.1371/journal.pone.0177943. DOI |
17 | Lillehaug, D. 1997. An improved plaque assay for poor plaque-producing temperate lactococcal bacteriophages. J Appl Microbiol 83: 85-90. DOI |
18 | Morin T, Martin H, Soumet C, Fresnel R, Lamaudie S, Le Sauvage AL, Deleurme K, Maris P. 2015. Comparison of the virucidal efficacy of peracetic acid, potassium monopersulphate and sodium hypochlorite on bacteriophages P001 and MS2. J Appl Microbiol 119: 655-665. DOI |
19 | Sanders ER. 2012. Aseptic Laboratory Techniques: Plating Methods. J Vis Exp 63: e3064. doi:10.3791/3064. DOI |
20 | Steinmann J. 2004. Surrogate viruses for testing virucidal efficacy of chemical disinfectants. J Hosp Infect 56: S49-S54. DOI |
21 | Ye Y, Ellenberg RM, Graham KE, Wigginton KR. 2016. Survivability, partitioning, and recovery of enveloped viruses in untreated municipal wastewater. Environ Sci Technol 50: 5077-5085. DOI |
![]() |