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http://dx.doi.org/10.7853/kjvs.2021.44.3.133

Comparison on prevalence and antimicrobial resistance of Pseudomonas spp. isolated from bovine mastitis milk in South Korea  

Kang, Hye Jeong (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Kim, Ha-Young (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Hong, Serim (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Park, Dasom (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Yoon, Soon-Seek (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Moon, Jin-San (Bacterial Disease Division, Animal and Plant Quarantine Agency)
Publication Information
Korean Journal of Veterinary Service / v.44, no.3, 2021 , pp. 133-140 More about this Journal
Abstract
This study was aimed to investigate the prevalence and antimicrobial resistance of Pseudomonas spp. isolated from bovine mastitis milk samples. A total of 50 (4.9%) Pseudomonas spp. was isolated from 1,023 samples, those collected between 2018 and 2021, derived from 110 dairy farms. The prevalence of the identified species of Pseudomonas isolates was as follows; P. aeruginosa (70.0%), P. fluorescens (14.0%), P. putida (10.0%), P. fragi (4.0%), and P. chlororaphis (2.0%). Most of somatic cell counts in the quarter milk carrying Pseudomonas spp. were less than 3,000,000 cell/ml (90.0%). The isolates of Pseudomonas spp. showed high susceptibility to cefepime (98.0%), ciprofloxacin (98.0%), ceftazidime (96.0%), and colistin (96.0%). The rate of antibiotic resistance in the isolates was highest to ceftiofur (92.0%), followed by the resistance rate to chloramphenicol (86.0%) and trimethoprim/sulphamethoxazole (80.0%). In addition, there is a remarkable difference in antimicrobial resistance pattern among Pseudomonas species. P. aeruginosa and P. putida showed a similar resistance pattern, whereas P. fluorescens showed exceptionally lower resistance to trimethoprim/sulphamethoxazole and chloramphenicol than that of the other species. This study showed that prevalence of Pseudomonas spp. other than P. aeruginosa were 30.0% in bovine mastitis milk, and the occurrence rate of antibiotic resistance were similar or higher level, compared with the previous reports on the mastitisderived Pseudomonas spp. isolated in Korea.
Keywords
Antimicrobial resistance; Bovine mastitis; Pseudomonas spp.; Somatic cell count;
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