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http://dx.doi.org/10.7853/kjvs.2021.44.2.103

Pathologic studies for porcine pleuropneumonia and serotypes of the agents in Jeju  

Kim, Ki-Seung (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Yang, Hyoung-Seok (Jeju Self-Governing Provincial Veterinary Research Institute)
Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Publication Information
Korean Journal of Veterinary Service / v.44, no.2, 2021 , pp. 103-111 More about this Journal
Abstract
Actinobacillus (A.) pleuropneumoniae is the etiological agent of a porcine pleuropneumonia and have great economic importance to the global swine industry. For recent 5 years, a total of 50 pleuropneumonia cases of 24 pig farms were selected from pig lungs submitted to the College of Veterinary Medicine, Jeju National University using polymerase chain reaction (PCR) analysis. Collected lungs were fixed in 10% neutral phosphate-buffered formalin and processed for histological examination. Serotypes of A. pleuropneumoniae in pneumonic lesions were analyzed by PCR methods. And the antimicrobial susceptibility of A. pleuropneumoniae isolates was determined by a disc diffusion test. Grossly, unilateral distribution of hemorrhagic or necrotic pneumonic lesions was more common than bilateral distribution in lungs. In peracute or acute cases, histopathologic changes were characterized by necrosis, hemorrhage, neutrophils infiltration, vascular thrombosis, widespread edema and fibrinous exudates. Following the acute response, macrophage infiltration, marked fibrosis around zonal necrotic areas, and marked fibrous pleuritis were characteristic in chronic cases. A total of 50 pleuropneumonia were associated with A. pleuropneumoniae serotype 5 in 46 cases (92%), serotype 2 in 3 cases (6%), and both 2 and 5 in 1 case (2%). More than 90% of collected isolates showed high sensitivity to ceftiofur, amoxicillin, and colistin. However, ampicillin, penicillin, and tylosin showed low susceptibility. The results of this study demonstrated that A. pleuropneumoniae serotype 5 was predominant at porcine pleuropneumonia cases in Jeju.
Keywords
Actinobacillus pleuropneumonia; Antimicrobial susceptibility; PCR; Porcine pleuropneumonia; Serotype;
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1 Schuchert JA, Inzana TJ, Angen O, Jessing S. 2004. Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR. J Clin Microbiol 42: 4344-4348.   DOI
2 Shin DH , Byun JW, Kim HY, Kim DK, Jang WN, Moon OK, Lee OS, Jung BY. 2011. Distribution of serotype and biofilm of Actinobacillus pleuropneumoniae isolated from pigs. Kor J Vet Publ Hlth 35: 7-12.
3 Ajito T, Haga Y, Homma S, Goryo M, Okada K. 1996. Immunohistological evaluation on respiratory lesions of pigs intranasally inoculated with Actinobacillus pleuropneumoniae serotype 1. J Vet Med Sci 58: 297-303.   DOI
4 Jung BY, Cho GJ, Kim BH, Cho KH. 1996. Biochemical characteristics and serotypes of Actinobacillus pleuropneumoniae isolated from pneumonic lungs of pigs. Korean J Vet Res 36: 181-186.
5 Lee JH, Jeong JY, Jeon YS, Seok HB. 1999. Studies on serotyping and detection of Actinobacillus pleuropneumoniae by Multiplex PCR techniques and immunodiffusion test. Kor J Anim Sci 41: 387-396.
6 Zhou L, Jones SC, Angen O, Bosse JT, Nash JH, Frey J, Zhou R, Chen HC, Kroll JS, Rycroft AN, Langford PR. 2008. Multiplex PCR that can distinguish between immunologically cross-reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains. J Clin Microbiol 46: 800-803.   DOI
7 Pijpers A, Vernooy JACM. van Leegoed LAMG, Verheijden JHM. 1990. Feed and water consumption in pigs following an Actinobacillus pleuropneumoniae challenge. Proc Int Congr Pig Vet Soc 11: 39.
8 Kamp EM, Popma JK, Anakotta J, Smits MA. 1991. Identification of hemolytic and cytotoxic proteins of Actinobacillus pleuropneumoniae by use of monoclonal antibodies. Infect Immun 59: 3079-3085.   DOI
9 Kim JA. Kim SI, Woo HC, Lim YK, Bae JH, Kim HS, Lee DS. 1998. Studies on biochemical characteristics and serotypes of Actinobacillus pleuropneumoniee isolated from pneumonic lungs of swine in Che-ju. Kor J Vet Publ Hlth 22: 147-155.
10 Marsteller TA, Fenwick B. 1999. Actinobacillus pleuropneumoniae disease and serology. Swine Health Prod 7: 161-165.
11 Yang CY, Lin CN, Lin CF, Chang TC, Chiou MT. 2011. Serotypes, antimicrobial susceptibility, and minimal inhibitory concentrations of Actionbacillus pleuropneumoniae isolated from slaughter pigs in Taiwan (2002-2007). J Vet Med Sci 73: 205-208.   DOI
12 Gorden WAM. 1963. The effect of housing on the degree and incidence of pneumonia in bacon pigs. Br Vet J 119: 307-314.   DOI
13 Asawa T, Kobayashi H, Mitani K, Ito N, Morozumi T. 1995. Serotypes and antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolated from piglets with pleuropneumonia. J Vet Med Sci 57: 757-759.   DOI
14 Frey J, Nicolet J. 1988. Purification and partial characterization of a hemolysin produced by Actinobacillus pleuropneumoniae type strain 4074. FEMS Microbiol Lett 55: 41-45.   DOI
15 Frey J. 1995. Virulence in Actinobacillus pleuropneumoniae and RTX toxins. Trends Microbiol 3: 257-261.   DOI
16 Gottschalk M, Taylor DJ. 2006. Actinobacillus pleuropneumoniae. pp. 563-576. In: Straw BE, Zimmerman JJ, D'Allaire S, Taylor DJ (eds), Diseases of swine, 9th ed. Blackwell Publishing, Ames, USA.
17 Angen O, Ahrens P, Jessing SG. 2008. Development of a multiplex PCR test for identification of Actinobacillus pleuropneumoniae serovars 1, 7, and 12. Vet Microbiol 132: 312-318.   DOI
18 Gutierrez-Martin CB, del Blanco NG, Blanco M, Navas J, Rodriguez-Ferri EF. 2006. Changes in antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolated from pigs in Spain during the last decade. Vet Microbiol 115: 218-222.   DOI
19 Jessing SG, Angen O, Inzana TJ. 2003. Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6. J Clin Microbiol 41: 4095-4100.   DOI
20 Jeong HK, Han JH. 2003. A pathological study in subsequent infection of PRRS virus to pigs with porcine pleuropneumoniae. Korean J Vet Pathol 7: 27-41.
21 Jeong HK, Han JH. Kim JH. 1996. Effect of relative humidity in swine house on pathogenesis of swine pleuropneumonia. Korean J Vet Res 36: 131-142.
22 Songer JG, Post WJ. 2005. Veterinary Microbiology: Bacterial and Fungal Agents of Animal Disease. pp. 174-180. Elsevier Saunders, St. Louis, Missouri, USA.