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http://dx.doi.org/10.7853/kjvs.2020.43.3.173

Comparison of PRRSV and antibody detection in oral fluid and serum samples from different age categories of PRRSV endemic farms  

Kim, Jung-Hee (Dodram Pig Farmers Cooperative Veterinary Clinic)
Son, Jae Guk (Muruki Farm)
Kim, Won-Il (College of Veterinary Medicine, Jeonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.43, no.3, 2020 , pp. 173-179 More about this Journal
Abstract
The objective of this study was to evaluate the usefulness of detection of PRRSV and PRRSV-specific antibodies in oral fluids for monitoring of PRRSV infection in endemic farms. The level of PRRSV and anti-PRRSV antibodies in serum and oral fluids was evaluated in five age groups of pigs (6, 9, 12, 16 weeks of age and gilts). The samples (25 serums and 5 oral fluids/per a farm) were collected from 5 different farms endemically infected by PRRSV. Both serum and oral fluid samples were tested for PRRSV by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and for anti-PRRSV antibodies by two commercial PRRSV ELISA kits. ELISA mean s/p ratios (2.98 vs 1.63) and positive rate (84.0% vs 68.8%) of the oral fluid samples showed significantly higher levels but had similar patterns to the seroprofile of the blood samples. The PRRSV positive rate of oral fluid and serum samples was 40.0% and 44.0% respectively. In conclusion, the use of oral fluids for PRRS monitoring in endemic farms is strongly recommended.
Keywords
Porcine reproductive and respiratory syndrome; Pen-based oral fluid; Serum samples; ELISA; qRT-PCR;
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1 Trang NT, Hirai T, Yamamoto T, Matsuda M, Okumura N, Giang NT, Lan NT and Yamaguchi R. 2014. Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd. J Vet Sci 15: 361-367.   DOI
2 Wills RW, Zimmerman JJ, Yoon KJ, et al. 1997a. Porcine reproductive and respiratory syndrome virus: a persistent infection. Vet Microbiol 55: 231-240.   DOI
3 Wills RW, Zimmerman JJ, Yoon KJ, et al. 1997b. Porcine reproductive and respiratory syndrome virus: routes of excretion. Vet Microbiol 57: 69-81.   DOI
4 Woonwong Y, Kedkovid R, Arunorat J, Sirisereewan C, Nedumpun T, Poonsuk K, Panyasing Y, Poolperm P, Boonsoongnern A, Thanawongnuwech R. 2018. Detection of PRRSV circulation using oral fluid samples for nursery management in endemically PRRSV-infected farms. Thai J Vet Med 48: 85-94.
5 Goodell CK, Prickett J, Kittawornrat A, Zhou F, Rauh R, Nelson W, O'Connell C, Burrell A, Wang C, Yoon KJ, Zimmerman JJ. 2013. Probability of detecting influenza A virus subtypes H1N1 and H3N2 in individual pig nasal swabs and pen-based oral fluid specimens over time. Veterinary Microbiology 166: 450-460.   DOI
6 Baron T, Albina E, Leforban Y, Madec F, Guilmoto H, Plana Duran J, Vannier P. 1992. Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation. Ann Rech Vet 23: 161-166.
7 Biernacka K, Karbowiak P, Wrobel P, Chareza T, Czopowicz M, Balka G, Goodell C, Rauh R, Stadejek T. 2016. Detection of porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) in oral fluid of pigs. Res Vet Sci 109: 74-80.   DOI
8 Cuong NV, Carrique-Mas J, Thu HTV, Hien ND, Hoa NT, Nguyet LA, Anh PH and Bryant JE. 2014. Serological and virological surveillance for porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and influenza A viruses among smallholder swine farms of the Mekong Delta, Vietnam. J Swine Health Prod 22: 224-231.
9 Decorte I, Campe WV, Mostin L, Cay AB, Regge ND. 2015. Diagnosis of the Lelystad strain of porcine reproductive and respiratory syndrome virus infection in individually housed pigs: comparison between serum and oral fluid samples for viral nucleic acid and antibody detection. J Vet Diagn Invest 27: 47-54.   DOI
10 Gerber PF, O'Neill K, Owolodun O, Wang, C, Harmon K., Zhang J, Halbur PG, Zhou L, Meng XJ, Opriessnig T. 2013. Comparison of commercial real-time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different sample types. J Clin Microbiol 51: 547-556.   DOI
11 Hirose O, Kudo H, Yoshizawa S, Hiroike T, Nakane T. 1995. Prevalence of porcine reproductive and respiratory syndrome virus in Chiba prefecture. J Jpn Vet Med Assoc 48: 650-653.   DOI
12 Kang H, Yu J, Shin J, Kang A, Kim W, Lee C, Lee J, Cho I. Choe S, Cha S. 2018. Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the republic of Korea between 2013 and 2016. BMC Vet Res 14: 160.   DOI
13 Holtkamp DJ, Polson DD, Torremorell M, Morrison B, Classen DM, Becton L, Henry S, Rodibaugh MT, Rowland RR, Snelson H, Straw B, Yeske P, Zimmerman J. 2011. Terminology for classifying the porcine reproductive and respiratory syndrome virus (PRRSV) status of swine herds. Tierarztl Prax Ausg G Grosstiere Nutztiere 39: 101-112.   DOI
14 Humphrey SP, Williamson RT. 2001. A review of saliva: normal composition, flow, and function. J Prosthet Dent 85: 162-169.   DOI
15 IDEXX. 2013. PRRS oral fluids Ab Test. Principle of oral fluid collection for the IDEXX PRRS oral Fluid Antibody Test. https://www.idexx.com/pdf/en_us/livestock-poultry/Oral-Fluids-FAQs.pdf. Accessed 22th October 2016.
16 Kittawornrat A, Panyasing Y, Goodell C, Wang C, Gauger P, Harmon K, Rauh R, Desfresne L, Levis I, Zimmerman J. 2014. Porcine reproductive and respiratory syndrome virus (PRRSV) surveillance using pre-weaning oral fluid samples detects circulation of wild-type PRRSV. Vet Microbiol 168: 331-339.   DOI
17 Kittawornrat A, Prickett J, Chittick W, Wang C, Engle M, Johnson J, Patnayak D, Schwartz T, Whitney D, Olsen C, Schwartz K, Zimmerman J. 2010. Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: Will oral fluid replace serum for PRRSV surveillance? Virus Res 154: 170-176.   DOI
18 Olsen C, Wang C, Christopher-Hennings J, Doolittle K, Harmon KM, Abate S, Kittawornrat A, Lizano S, Main R, Nelson EA, Otterson T, Panyasing Y, Rademacher C, Rauh R, Shah R, Zimmerman J. 2013. Probability of detecting Porcine reproductive and respiratory syndrome virus infection using pen-based swine oral fluid specimens as a function of within-pen prevalence. J Vet Diagn Invest 25: 328-335.   DOI
19 Kuhn JH, Lauck M, Bailey AL, Shchetinin AM, Vishnevskaya TV, Bao Y, Ng TF, LeBreton M, Schneider BS, Gillis A, Tamoufe U, Diffo JLD, Takuo JM, Kondov NO, Coffey LL, Wolfe ND, Delwart E, Clawson AN, Postnikova E, Bollinger L, Lackemeyer MG, Radoshitzky SR, Palacios G, Wada J, Shevtsova ZV, Jahrling PB, Lapin BA, Deriabin PG, Dunowska M, Alkhovsky SV, Rogers J, Friedrich TC, O'Connor DH, Goldberg TL. 2016. Reorganization and expansion of the nidoviral family Arteriviridae. Arch Virol 161: 755-768.   DOI
20 McKie A, Vyse A, Maple C. 2002. Novel methods for the detection of microbial antibodies in oral fluid. The Lancet Infectious Diseases, 2: 18-24.   DOI
21 Pepin BJ, Kittawornrat A, Liu F, Gauger PC, Harmon K, Abate S, Main R, Garton C, Hargrove J, Rademacher C, Ramirez A, Zimmerman J. 2015. Comparison of specimens for detection of porcine reproductive and respiratory syndrome virus infection in boar studs. Transbound Emerg Dis 62: 295-304.   DOI
22 Prickett JR, Kim WI, Simer R, Yoon KJ, Zimmerman J. 2008. Oral-fluid samples for surveillance of commercial growing pigs for porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 infections. J Swine Health Prod. 16: 86-91.
23 Regge ND and Cay B. 2016. Comparison of PRRSV nucleic acid and antibody detection in pen-based oral fluid and individual serum samples in three different age categories of post-weaning pigs from endemically infected farms. PLoS One 11: e0166300.   DOI