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http://dx.doi.org/10.7853/kjvs.2020.43.2.53

Prevalence of enterovirulent Escherichia coli from diarrhea of cattles in Jeonbuk, Korea  

Jeong, Hansol (Jeollabuk-do Institute of Livestock & Veterinary Research)
Baek, Kui-Jeong (Jeollabuk-do Institute of Livestock & Veterinary Research)
Koh, Won-Seok (Jeollabuk-do Institute of Livestock & Veterinary Research)
Lee, Jeong-Won (Jeollabuk-do Institute of Livestock & Veterinary Research)
Jeong, Jae-Kyo (Jeollabuk-do Institute of Livestock & Veterinary Research)
Publication Information
Korean Journal of Veterinary Service / v.43, no.2, 2020 , pp. 53-58 More about this Journal
Abstract
Enterovirulent Escherichia coli are among the most important causes of diarrhea in cattles. Between January and December, 2017, a total of 150 stool specimens from cattles were investigated for enterohaemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC) and enteroinvasive E. coli (EIEC) using real-time PCR. 131 E. coli were isolated from feces. The most frequently isolated pathotype in feces was EHEC (37 isolates). EPEC, ETEC and EAEC were detected in feces with 14, 7 and 3 respectively. EIEC was not detected. Antimicrobial resistance test was performed by agar disc diffusion method with 14 antimicrobials. Enterovirulent E. coli isolates showed the highest antimicrobial resistance to ampicillin 61.3%, followed by tetracycline 54.5% and streptomycin 45.5%. They had low resistance to amikacin 11.4%. Of 44 isolates, 37 (84.1%) were resistant to more than 2 antimicrobials. futher study a highest antimicrobial susceptibility to trimethoprim/sulfamethoxazole 50.0% and florofenicol 47.7%.
Keywords
Escherichia coli; Cattles; Real-time PCR; Antimicrobial resistance;
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