Browse > Article
http://dx.doi.org/10.7853/kjvs.2019.42.4.257

Protective efficacy of formalin-inactivated Salmonella Gallinarum whole cells vaccine using mastoparan V1 as adjuvant against fowl typhoid  

Moon, Ja-Young (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Kwak, Kil Han (Animal Health Institute of Jeollabukdo)
Ochirkhuyag, Enkhsaikhan (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Kim, Seon-Min (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Lee, Jun-Woo (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Jo, Young-Gyu (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Kim, Won-Kyong (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Bang, Woo Young (National Institute of Biological Resources, Ministry of Environment)
Bae, Chang Hwan (National Institute of Biological Resources, Ministry of Environment)
Hur, Jin (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.42, no.4, 2019 , pp. 257-264 More about this Journal
Abstract
Mastoparan V1 was used as adjuvant of formalin-inactivated Salmonella Gallinarum whole cells vaccine against fowl typhoid in a chicken model. The 75 brown nick chickens were equally divided into 5 groups, and all chickens of each group were immunized at 6 weeks of age (0 WPPI; weeks prime post immunization), and at 9 weeks of age (3 WPPI) (except group B). Group A chickens were intramuscularly (IM) inoculated with 500 uL of sterile phosphate-buffered saline (PBS), and group B chickens were subcutaneously immunized with 0.2 ml containing 5×107 viable vaccine strain/bird. The chickens in groups C~E were IM inoculated with approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells, approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells with mastoparan V1 as adjuvant, and 0.5 mL of PBS, respectively. S. Gallinarum outer membrane proteins-specific serum IgG titers were considerably higher in groups B~D than in groups A and E. However, the levels of IFN-γ in groups B and D only than in groups A and E were significantly higher. Following oral challenge with virulent wild-type S. Gallinarum, no chicken in groups A (no challenge group) and B was dead, and only 30% of chickens in group D was dead. However, 70% of chickens in group C and all chickens in group E were dead after oral challenge. The results of this study demonstrated that IM immunization with approximately 3×109 of the formalin-inactivated S. Gallinarum whole cells containing mastoparan V1 induced robust antibody and cell-mediated immune responses in chickens. The whole cells also conferred protection against infection with wild-type S. Gallinarum.
Keywords
Fowl typhoid; Chicken; Salmonella Gallinarum; Mastoparan V1; Vaccine;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jawale CV, Chaudhari AA, Lee JH. 2014. Generation of a safety enhanced Salmonella Gallinarum ghost using antibiotic resistance free plasmid and its potential as an effective inactivated vaccine candidate against fowl typhoid. Vaccine 32(9): 1093-9.   DOI
2 Jawale CV, Lee JH. 2014. A novel approach for the generation of Salmonella Gallinarum ghostsand evaluation of their vaccine potential using a prime-boosterimmunization strategy. Vaccine 32: 6776-6782.   DOI
3 Kang MS, Kwon YK, Jung BY, Kim A, Lee KM, An BK, Song EA, Kwon JH, Chung GS. 2011. Differential identification of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum based on polymorphic regions of glgC and speC genes. Vet Microbiol 147(1-2): 181-5   DOI
4 Kim NH, Ha EJ, Ko DS, Lee CY, Kim JH, Kwon HJ. 2019. Molecular evolution of Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum in the field. Vet Microbiol 235: 63-70.   DOI
5 Kim Y, Son M, Noh E-Y, Kim S, Kim C, Yeo J-H, Park C, Lee KW, Bang WY. 2016. MP-V1 from the venom of social wasp Vespula vulgaris is a de novo type of mastoparan that displays superior antimicrobial activities. Molecules 21: 512.   DOI
6 King TP, Jim SY, Wittkowski KM. 2003. Inflammation role of two venom components of yellow jackets (Vespula vulgaris): a mast cell degranulating peptide mastoparan and phospholipase A1. Int Arch Allergy Immunol 131(1): 25-32.   DOI
7 Koehbach J, Craik DJ. 2019. The vast structural diversity of antimicrobial peptides. Trends Pharmacol Sci 40: 517-528.   DOI
8 Koerich PKV, Fonseca BB, Balestrin E, Tagliari V, Hoepers PG, Ueira-Vieira C, Oldoni I, Rauber RH, Ruschel L, Nascimento VP. 2018. Salmonella Gallinarum field isolates and its relationship to vaccine strain SG9R. Br Poult Sci 59(2): 154-159.   DOI
9 Powers J-PS, Hancock REW. 2003. The relationship between peptide structure and antibacterial activity. Peptides 24: 1681-1691.   DOI
10 Ramirez-Carreto S, Jimenez-Vargas JM, Rivas-Santiago B, Corzo G, Possani LD, Becerril B, Ortiz E. 2015. Peptides from the scorpion Vaejovis punctatus with broad antimicrobial activity. Peptides 73: 51-59.   DOI
11 Seo KW, Kim JJ, Mo IP, Lee YJ. 2019. Molecular characteristic of antimicrobial resistance of Salmonella Gallinarum isolates from chickens in Korea, 2014 to 2018. Poult Sci 98(11): 5416-5423.   DOI
12 Setta AM, Barrow PA, Kaiser P, Jones MA. 2012. Early immune dynamics following infection with Salmonella enterica serovars Enteritidis, Infantis, Pullorum and Gallinarum: cytokine and chemokine gene expression profile and cellular changes of chicken cecal tonsils. Comp. Immunol Microbiol Infect Dis 35: 397-410.   DOI
13 Shivaprasad HL. 2000. Fowl typhoid and pullorum disease. Rev Sci Tech 19: 405-424.   DOI
14 Silva EN, Snoeyenbos GH, Weinack OM, Smyser CF. 1981. Studies on the use of 9R strain of Salmonella gallinarum as a vaccine in chickens. Avian Dis 25: 38-52.   DOI
15 Van Immerseel F, Studholme DJ, Eeckhaut V, Heyndrickx M, Dewulf J, Dewaele I, Van Hoorebeke S, Haesebrouck F, Van Meirhaeghe H, Ducatelle R, Paszkiewicz K, Titball RW. 2013. Salmonella Gallinarum field isolates from laying hens are related to the vaccine strain SG9R. Vaccine 31(43): 4940-5.   DOI
16 Wang K, Li Y, Xia Y, Liu C. 2016. Research on Peptide Toxins with Antimicrobial Activities. Ann Pharmacol Pharm 1: 1006.
17 Machado RJA, Estrela AB, Nascimento AKL, Melo MMA, Torres-Rego M, Lima EO, Rocha HAO, Carvalho E, Silva-Junior AA, Fernandes-Pedrosa MF. 2016. Characterization of TistH, a multifunctional peptide from the scorpion Tityus stigmurus: Structure, cytotoxicity and antimicrobial activity. Toxicon 119: 362-370.   DOI
18 Kuhn-Nentwig, L. 2003. Antimicrobial and cytolytic peptides of venomous arthropods. Cell Mol Life Sci 60: 2651-2668.   DOI
19 Kwon HJ, Cho SH. 2011. Pathogenicity of SG 9R, a rough vaccine strain against fowl typhoid. Vaccine 29: 1311-1318.   DOI
20 Lee SK, Chon JW, Song KY, Hyeon JY, Moon JS, Seo KH. 2013. Prevalence, characterization, and antimicrobial susceptibility of Salmonella Gallinarum isolated from eggs produced in conventional or organic farms in South Korea. Poult Sci 92: 2789-2797.   DOI
21 Mastroeni P, Chabalgoity JA, Dunstan SJ, Maskell DJ, Dougan G. 2001. Salmonella: immune responses and vaccines. Vet J 161: 132-164.   DOI
22 Matsuda K, Chaudhari AA, Kim SW, Lee KM, Lee JH. 2010. Physiology, pathogenicity and immunogenicity of lon and/or cpxR deleted mutants of Salmonella gallinarum as vaccine candidates for fowl typhoid. Vet Res 41: 59.   DOI
23 Moon JY, Kim SY, Kim WK, Rao Z, Park JH, Mun JY, Kim B, Choi HS, Hur J. 2017. Protective efficacy of a Salmonella Typhimurium ghost vaccine candidate constructed with a recombinant lysozyme-PMAP36 fusion protein in a murine model. Can J Vet Res 81(4): 297-303.
24 Oguiura N, Boni-Mitake M, Affonso R, Zhang G. 2011. In Vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus. J Antibiot 64: 327-331.   DOI
25 Ha YJ, Kim SW, Lee CW, Bae CH, Yeo JH, Kim IS, Gal SW, Hur J, Jung HK, Kim MJ, Bang WY. 2017. Anti-Salmonella activity modulation of mastoparan V1-a aasp venom toxin-using protease inhibitors, and its efficient production via an Escherichia coli secretion system. Toxins (Basel). 9: 321.   DOI
26 Wigley P. 2017. Salmonella enterica serovar Gallinarum: addressing fundamental questions in bacteriology sixty years on from the 9R vaccine. Avian Pathol 46: 119-124   DOI
27 Won G, Chaudhari AA, Lee JH. 2016. Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated Salmonella Gallinarum vaccine candidate. Clin Exp Vaccine Res 5(2): 148-58.   DOI
28 Yeung AT, Gellatly SL, Hancock RE. 2011. Multifunctional cationic host defence peptides and their clinical applications. Cell Mol Life Sci 68: 2161-2176.   DOI
29 김성국, 김영환, 엄현정, 장성준, 조광현, 이양수. 2006. 육계에서 분리한 Salmonella gallinarum의 약제내성 및 PFGE 양상. 한국가축위생학회지 29: 297-308.
30 Fratini F, Cilia G, Turchi B, Felicioli A. 2017. Insects, arachnids and centipedes venom: A powerful weapon against bacteria. A literature review. Toxicon 130: 91-103.   DOI
31 Hajam IA, Kim J, Lee JH. 2018. Oral immunization with a novel attenuated Salmonella Gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens. Vet Res 12;49(1): 91.   DOI
32 Hajam IA, Kim JH, Lee JH. 2018. Incorporation of membrane-anchored flagellin into Salmonella Gallinarum bacterial ghosts induces early immune responses and protection against fowl typhoid in young layer chickens. Vet Immunol Immunopathol 199: 61-69.   DOI
33 Hetru C, Letellier L, Ziv O, Hoffmann JA, Yechiel S. 2000. Androctonin, a hydrophilic disulphide-bridged non-haemolytic anti-microbial peptide: A plausible mode of action. Biochem J 345: 653-664.   DOI
34 Hur J1, Song SO, Lim JS, Chung IK, Lee JH. 2011. Efficacy of a novel virulence gene-deleted Salmonella Typhimurium vaccine for protection against Salmonella infections in growing piglets. Vet Immunol Immunopathol 139(2-4): 250-256.   DOI
35 Ibe MI, Odimegwu DC, Onuigbo EB. 2019. Alginate-coated chitosan microparticles encapsulating an oral plasmid-cured live Salmonella enterica serovar Gallinarum vaccine cause a higher expression of interfron-gamma in chickens compared to the parenteral live vaccine. Avian Pathology 48(5): 423-428.   DOI
36 Bouzoubaa K, Nagaraja KV, Kabbaj FZ, Newman JA, Pomeroy BS. 1989. Feasibility of using proteins from Salmonella gallinarum vs. 9R live vaccine for the prevention of fowl typhoid in chickens. Avian Dis 33: 385-391.   DOI
37 김기석, 이희수, 모인필. 1995. 국내 닭에서의 가금티푸스 발생. 농촌진흥청 농업과학논문집 37(1): 544-549.
38 배종철, 김성국, 김영환, 조민희, 이영주*, 박청규. 2009. 닭에서 분리한 Salmonella Gallinarum의 약제내성 및 PFGE, 한국가축위생학회지 32:155-163.
39 이동석, 한태욱. 2000. 국내에서 분리한 Salmonella gallinarum의 병원성, 항생제 감수성 및 plasmid profile. 한국수의공중보건학회지 24(1): 49-57.
40 Arora D, Kumar S, Jindal N, Narang G, Kapoor PK, Mahajan NK, 2015. Prevalence and epidemiology of Salmonella enterica serovar Gallinarum from poultry in some parts of Haryana, India. Vet World 8: 1300-1304.   DOI
41 Bulet P, Stocklin R, Menin L. 2004. Anti-microbial peptides: From invertebrates to vertebrates. Immunol Rev 198:169-184.   DOI
42 Chen LW, Kao PH, Fu YS, Lin SR, Chang LS. 2011. Membranedamaging activity of Taiwan cobra cardiotoxin 3 is responsible for its bactericidal activity. Toxicon 58: 46-53.   DOI
43 Cheng Z, Yin J, Kang X, Geng S, Hu M, Pan Z, Jiao X. 2016. Safety and protective efficacy of a spiC and crp deletion mutant of Salmonella gallinarum as a live attenuated vaccine for fowl typhoid. Res Vet Sci 107: 50-54.   DOI
44 Desin TS, Koster W, Potter AA, 2013. Salmonella vaccines in poultry: past, present, and future. Expert Rev Vaccines. 12: 87-96.   DOI
45 Fashae K, Ogunsola F, Aarestrup FM, Hendriksen RS. 2010. Antimicrobial susceptibility and serovars of Salmonella from chickens and humans in Ibadan, Nigeria. J Infect Dev Ctries 4: 484-494   DOI