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http://dx.doi.org/10.7853/kjvs.2018.41.4.229

Detection of methicillin-resistant S. pseudintermedius (MRSP) isolated from dogs and cats in Kyungpook Area  

Byun, Jun Ho (North Branch, Gyeongbuk Veterinary Service Laboratory)
Sohn, Jun Hyung (North Branch, Gyeongbuk Veterinary Service Laboratory)
Lee, Young Mi (North Branch, Gyeongbuk Veterinary Service Laboratory)
Jeong, Hyo Hoon (College of Veterinary Medicine, Kyungpook National University)
Oh, Tae Ho (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Service / v.41, no.4, 2018 , pp. 229-237 More about this Journal
Abstract
Identification of antibiotic resistant bacteria isolated from animals is necessary for seeking a proper treatment and for preventing the spread of the bacteria among animals. Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. This study was conducted to investigate the antimicrobial susceptibility of Staphylococcus pseudintermedius (S. pseudintermedius) isolated from dogs and cats. Out of the total number of 150 specimens, 35 isolates (23.3%) were identified as S. pseudintermedius when tested by MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy). Among them, 5 isolates (14.3%) were the case of MRSP. In the results of the antimicrobial susceptibility test, the isolates of S. pseudintermedius were susceptible to amikacin (100%), cephalothin (100%), vancomycin (100%), amoxicillin (85.7%), oxacillin (85.7%), enrofloxacin (82.8%), chloramphenicol (80%). On the other hand, they were resistant to penicillin (71.4%), tetracycline (48.6%), ampicillin (37.1%), kanamycin (31.5%), erythromycin (31.4%), respectively.
Keywords
Staphylococcus pseudintermedius; MRSP; Antimicrobial susceptibility; MALDI-TOF;
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1 Wang Y, Yang J, Logue CM, Liu K, Cao X, Zhang W, Shen J, Wu C. 2012. Methicillin-resistant Staphylococcus pseudintermedius isolated from canine pyoderma in North China. J Appl Microbiol 112: 623-630.   DOI
2 Weese JS, van Duijkeren E. 2010. Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Vet Microbiol. 140: 418-429.   DOI
3 Wettstein K, Descloux S, Rossano A, Perreten V. 2008. Emergence of methicillin-resistant Staphylococcus pseudintermedius in Switzerland: three cases of urinary tract infections in cats. Schweiz Arch Tierheilkd. 150: 339-343.   DOI
4 Yoo JH, Yoon JW, Lee SY, Park HM. 2010. High prevalence of Fluoroquinolone- and Methicillin- resistant Staphylococcus pseudintermedius isolates from canine pyoderma and otitis externa in veterinary teaching hospital. J Microbiol Biotechnol 20: 798-802.
5 Yoon JW, Lee KJ, Lee SY, Chae MJ, Park JK, Yoo JH, Park HM. 2010. Antibiotic resistance profiles of Staphylococcus pseudintermedius isolates from canine patients in Korea. J Microbiol Biotechnol 20: 1764-1768.
6 Zur G, Gurevich B, Elad D. 2016. Prior antimicrobial use as a risk factor for resistance in selected Staphylococcus pseudintermedius isolates from the skin and ears of dogs. Vet Dermatol. 27: 468-e125.   DOI
7 Anhalt JP, Fenselau C. 1975. Identification of bacteria using mass spectrometry. Anal. Chem. 47: 219-225.   DOI
8 Barbuddhe SB, Maier T, Schwarz G, Kostrzewa M, Hof H, Domann E, Chakraborty T, Hain T. 2008. Rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl Environ Microbiol 74: 5402-5407.   DOI
9 Bardiau M, Yamazaki K, Ote I, Misawa N, Mainil JG. 2013. Characterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats. Microbiol Immunol 57: 496-501.
10 Cho JK, Lee MR, Kim JM, Kim HD. 2016. Methicillin-resistant or susceptible Staphylococcus pseudintermedius isolates from dogs and cats. Korean J Vet Serv 39: 175-181.   DOI
11 Clinial and Laboratory Standards Institute (CLSI). 2014. Performance standards for antimicrobial susceptibility testing; Twenty-Fourth informational supplement. M100-S24. Clinical and Laboratory Standards Insitute. 34: 68-75.
12 Devriese LA, Vancanneyt M, Baele M, Vaneechoutte M, De Graef E, Snauwaert C, Cleenwerck I, Dawyndt P, Swings J, Decostere A, Haesebrouck F. 2005. Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals. Int J Syst Evol Microbiol. 55: 1569-1573.   DOI
13 Dziva F, Wint C, Auguste T, Heeraman C, Dacon C, Yu P, Koma LM. 2015. First identification of methicillin-resistant Staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in Trinidad, West Indies. Infect Ecol Epidemiol. 5: 29170.   DOI
14 Holland RD, Wilkes JG, Ralli F, Sutherland JB, Persons CC, Voorhees KJ, Lay Jr. JO. 1996. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry. Rapid Commun. Mass Sp. 10: 1227-1232   DOI
15 Faller A, Schleifer KH. 1981. Modified oxidase and benzidine tests for separation of staphylococci from micrococci. J Clin Microbiol 13: 1031-1035.
16 Feng Y, Tian W, Lin D, Luo Q, Zhou Y, Yang T, Deng Y, Liu YH, Liu JH. 2012. Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius in pets from South China. Vet Microbiol 160: 517-524.   DOI
17 Gold RM, Cohen ND, Lawhon SD. 2014. Amikacin Resistance in Staphylococcus pseudintermedius Isolated from Dogs. J Clin Microbiol. 52: 3641-3646.   DOI
18 Gronthal T, Eklund M, Thomson K, Piiparinen H, Sironen T, Rantala M. 2017. Antimicrobial resistance in Staphylococcus pseudintermedius and the molecular epidemiology of methicillin-resistant S. pseudintermedius in small animals in Finland. J Antimicrob Chemother. 72: 1021-1030.
19 Haenni M, de Moraes NA, Chatre P, Medaille C. 2014. Characterisation of clinical canine meticillin-resistant and meticillin-susceptible Staphylococcus pseudintermedius in France. J Global Antimicrobial Resistance 2: 119-123.   DOI
20 Kang MH, Chae MJ, Yoon JW, Kim SG, Lee SY, Yoo JH, Park HM. 2014. Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs. J Vet Sci 15: 409-415.   DOI
21 Khot PD, Couturier MR, Wilson A, Croft A, Fisher MA. 2012. Optimization of matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis for bacterial identification. J Clin Microbiol 50: 3845-3852.   DOI
22 Kim HW, Han JS, Seol KH, Han SH, Park BY, Oh MH. 2012. MALDI-TOF MS system for the identification of Microorganisms in Milk and Dairy Product. Korean J Dairy Sci Technol 30: 131-137.
23 Konnerth S, Rademacher G, Suerbaum S, Ziesing S, Sedlacek L, Vonberg RP. 2014. Identification of pathogens from blood culture bottles in spiked and clinical samples using matrix-assisted laser desorption ionization time-off-light mass-spectrometry analysis. BMC Res Notes 27: 405.
24 Krishnamurthy T, Ross PL, Rajamani U. 1996. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Rapid Commun. Mass Sp. 10: 883-888   DOI
25 Kristina Kadlec, Stefan Schwarz. 2012. Antimicrobial resistance of Staphylococcus pseudintermedius. Vet Dermatol. 23: 276-282.   DOI
26 Kudirkiene Egle, Welker Martin, Knudsen Nanna R, Bojesen Anders M. 2015. Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS. Syst Appl Microbiol 38: 315-322.   DOI
27 Lozano C, Rezusta A, Ferrer I, Perez-Laguna V, Zarazaga M, Ruiz-Ripa L, Revillo MJ, Torres C. 2016. Staphylococcus pseudintermedius Human Infection Cases in Spain: Dog-to-Human Transmission.
28 Nienhoff U, Kadlec K, Chaberny IF, Verspohl J, Gerlach GF, Kreienbrock L, Schwarz S, Simon D, Nolte I. 2011. Methicillin-resistant Staphylococcus pseudintermedius among dogs admitted to a small animal hospital. Vet Microbiol 150: 191-197.   DOI
29 Normand EH, Gibson NR, Reid SW, Carmichael S, Taylor DJ. 2000. Antimicrobial-resistance trends in bacterial isolates from companion-animal community practice in the UK. Prev Vet Med 46: 267-278.   DOI
30 Norstrom M, Sunde M, Tharaldsen H, Mork T, Bergsjo B, Kruse H. 2009. Antimicrobial Resistance in Staphylococcus pseudintermedius in the Norwegian Dog Population. Microb Drug Resist. 15: 55-59.   DOI
31 Onuma K, Tanabe T, Sato H. 2011. Antimicrobial resistance of Staphylococcus pseudintermedius isolates from healthy dogs and dogs affected with pyoderma in Japan. Vet Dermatol. 23: 17-22.
32 Pellerin JL, Bourdeau P, Sebbag H, Person JM. 1998. Epidemiosurveillance of antimicrobial compound resistance of Staphylococcus intermedium clinical isolates from canine pyodermas. Comp Immunol Microbiol Infect Dis. 21: 115-133.   DOI
33 Perreten V, Kadlec K, Schwarz S, Gronlund Andersson U, Finn M, Greko C, Moodley A, Kania SA, Frank LA, Bemis DA, Franco A, Iurescia M, Battisti A, Duim B, Wagenaar JA, van Duijkeren E, Weese JS, Fitzgerald JR, Rossano A, Guardabassi L. 2010. Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study. J Antimicrob Chemother. 65: 1145-1154.   DOI
34 Priyantha R, Gaunt MC, Rubin JE. 2016. Antimicrobial susceptibility of Staphylococcus pseudintermedius colonizing healthy dogs in Saskatoon, Canada. Can Vet J 57: 65-69.
35 Rubin JE, Chirino-Trejo M. 2011. Prevalence, Sites of Colonization, and Antimicrobial Resistance Among Staphylococcus Pseudintermedius Isolated from Healthy Dogs in Saskatoon, Canada. J Vet Diagn Invest 23: 351-354.   DOI
36 Sohn JH, Kim YH, Shin SH, Lee EM, Kim ST, Cho MH, Yun MJ. 2014. Characteristics and antimicrobial resistance patterns of Pasteurella multocida isolated from swine in Gyeongbuk province. Korean J Vet Serv 37: 165-171.   DOI
37 Ruscher C, Lubke-Becker A, Semmler T, Wleklinski CG, Paasch A, Soba A, Stamm I, Kopp P, Wieler LH, Walther B. 2010. Widespread rapid emergence of a distinct methicillin- and multidrug-resistant Staphylococcus pseudintermedius (MRSP) genetic lineage in Europe. Vet Microbiol 144: 340-346.   DOI
38 Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatsu K. 2007. Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital. J Clin Microbiol 45: 1118-1125.   DOI
39 Shimizu A, Wakita Y, Nagase S, Okabe M, Koji T, Hayashi T, Nagase N, Sasaki A, Kawano J, Yamashita K, Takagi M. 2001. Antimicrobial susceptibility of Staphylococcus intermedius isolated from healthy and diseased dogs. J Vet Med Sci 63: 357-360.   DOI
40 Szabados F, Woloszyn J, Richter C, Kaase M, Gatermann S. 2010. Identification of molecularly defined Staphylococcus aureus strains using matrix-assisted laser desorption/ionization time of flight mass spectrometry and the Biotyper 2.0 database. J Med Microbiol 59: 787-790.   DOI
41 Timoney JF, Gillespie JH, Scott FW, Barlough JE. 1988. The Genus Staphylococcus. In: Hagan and Bruner's Microbiology and Infectious Disease of Domestic Animals. 8 eds. Comstock Publishing Associates, Ithaca and London: 171-196.
42 van Duijkeren E, Catry B, Greko C, Moreno MA, Pomba MC, Pyorala S, Ruzauskas M, Sanders P, Threlfall EJ, Torren-Edo J, Torneke K; Scientific Advisory Group on Antimicrobials (SAGAM). 2011. Review on methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother. 66: 2705-2714.   DOI