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http://dx.doi.org/10.7853/kjvs.2018.41.3.149

Analysis of antibiotic susceptibility of Salmonella Enteritidis isolated from Gyeongnam province and the bacterial genotyping by using RAPD-PCR  

Kim, Eun-Gyeong (Gyeongnam Veterinary Service Laboratory)
Kim, Min-Kyung (Gyeongnam Veterinary Service Laboratory)
Kwon, Hyun-Ae (Gyeongnam Veterinary Service Laboratory)
Youn, Do-Kyung (Gyeongnam Veterinary Service Laboratory)
Koo, Jeong-Heon (Gyeongnam Veterinary Service Laboratory)
Park, So-Yeon (Gyeongnam Veterinary Service Laboratory)
Lee, Hui-Geun (Gyeongnam Veterinary Service Laboratory)
Jo, Myeong-Hui (Gyeongnam Veterinary Service Laboratory)
Hah, Do-Yun (Gyeongnam Veterinary Service Laboratory)
Kim, Cheol-Ho (Gyeongnam Veterinary Service Laboratory)
Hwang, Bo-Won (Gyeongnam Veterinary Service Laboratory)
Kim, Sang-Hyun (Laboratory of Veterinary Microbiology, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Service / v.41, no.3, 2018 , pp. 149-155 More about this Journal
Abstract
Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400~3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.
Keywords
Salmonella Enteritidis; Antibiotic resistance pattern; RAPD-PCR; Genotyping;
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