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http://dx.doi.org/10.7853/kjvs.2015.38.3.173

QuEChERS-based determination of tissue residues and acute toxicity of pyraclofos in rat  

Pyo, Min-Jung (Institute of Animal Medicine, College of Vetrinary Medicine, Gyeongsang National University)
Hah, Do-Yun (Gyeongnam Livestock Promotion Research Institue)
Choi, You-Jeong (Gyeongnam Livestock Promotion Research Institue)
Jeong, Kwi-Ok (Gyeongnam Livestock Promotion Research Institue)
Han, Chang-Hee (Gyeongnam Livestock Promotion Research Institue)
Park, Young-Ho (Gyeongnam Livestock Promotion Research Institue)
Kim, Min-Hee (Gyeongnam Livestock Promotion Research Institue)
Kim, Won-Gyu (Gyeongnam Livestock Promotion Research Institue)
Jung, Jing-Gune (Gyeongnam Livestock Promotion Research Institue)
Kim, Munki (Department of Anatomy, College of Vetrinary Medicine, Gyeongsang National University)
Kim, Euikyung (Institute of Animal Medicine, College of Vetrinary Medicine, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Service / v.38, no.3, 2015 , pp. 173-180 More about this Journal
Abstract
Environmental pesticides used for insect control can be transferred from plants to animals even to livestock animals through food chain. Human beings also can be exposed to pesticides by consuming polluted dairy products, including meats, eggs and other milk products. Therefore, the Ministry of Food and Drug Safety (MFDS) established Standard for Pesticide Residue Limits in dairy products. The QuEChERS (quick, easy, cheap, effective, rugged and safe) methods for detecting residual pesticides are relatively well established for fruits and vegetables, however, the methods for meat have not been appropriately studied yet. In the present work, pyraclofos was used as an organophosphate pesticide to examine its tissue residue in experimental animals by QuEChERS methods. For this, pyraclofos (150 mg/kg body weight) was orally administered to male rats once a day for 2 days. After 6, 12, and 24 hr of the treatment, the tissue residues in liver and femoral muscle of the rats were determined using QuEChERS methods followed by HPLC analyses. In preliminary studies, the recovery rates of spiking samples of pyraclofos demonstrated approximately 109~110% from the tissues. In previous study, pyraclofos tissue residues were observed with significantly high levels in livers and muscles at 6 hr of oral treatment. Then, they were almost completely disappeared after 24 hr of the administration, indicating the orally exposed pyraclofos is rapidly absorbed and distributed to body organs, then quickly excreted from the body with a negligible level of tissue residue. The alterations in blood chemistry as well as the histopathology of heart, lung, liver, spleen and kidney have also been investigated in the experimental animals for assessing acute toxic effects of pyraclofos. The obtained blood chemistry indexes (ALT and AST) showed maximum peak values at 12 hr after the oral administration and decreased to the normal levels at 24 hr of the treatment. Histopathologic observation exhibited acute hepatic damages at 24 hr of the treatment. In conclusion, we suggest that QuEChERS method can be adequately optimized for the analysis of pyraclofos residues in animal tissues.
Keywords
QuEChERS method; Pyraclofos; Tissue residue; Acute toxicity; HPLC;
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1 한국작물보호협회. 2013. 농약연보. 한국작물보호협회. 521.3059농 872.
2 Codex Alimentarius Commission. 2010. Pesticide Residues in Food. Rome: FAO/WHO Food Standard. Available from ; http://www.fao.org/docrep/012/i1400e/i1400e.pdf
3 Ecobichon, D.J. 1991. Pesticides. In : Amdur M.O., Doull, J. and Klaassen, C.D. (Eds.), Casarett and Doull's Toxicology; The Basic Science of Posions. Perganmo Press, New York, pp. 580-592.
4 Edwards R, Millburn P, Hutson DH. 1986. Comparative toxicity of cis-cypermethrin in rainbow trout, frog, mouse, and quail. Toxicol Appl Pharmacol 84: 512-522.   DOI
5 Fishel, F. M. 2007. Pesticide use trends in the US: Global comparison. Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Gainesville.
6 Kono Y, Manabe Y and Sato Y. 1986. Relation of AChE and AliE inhibition of anti-AChE insecticides, TIA-230, fenitrothion and carbaryl to their residual activity in Sodoptera litura larvae (Lepidoptera: Noctuidae). Applied Entomology and Zoology 21: 363-369.   DOI
7 Korea Health Industry Development Institute. KHIDI. 2011. MRLs for pesticides in Foods. 87-88.
8 LeDoux M. 2011. Analytical methods applied to the determination of pesticide residues in foods of animal origin. A review of the past two decades. J chromatogr A. 1218: 1021-1036.   DOI   ScienceOn
9 Lehotay SJ. 2007. Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study. J AOAC Int l90: 485-520.
10 Park JY, Choi JH, Abd El-Aty AM, Kim BM, Oh JH, Do JA, Kwon KS, Shim KH, Choi OJ, Shin SC, Shim JH. 2011. Simultaneous multiresidue analysis of 41 pesticide residues in cooked foodstuff using QuEChERS: Comparison with classical method. Food Chem 128: 241-253.   DOI   ScienceOn
11 Wilkowska A, Biziuk M. 2011. Determination of pesticide residues in food matrices using the QuEChERS methodology. Food Chem 125: 803-812.   DOI   ScienceOn
12 Paya P, Anastassiades M, Mack D, Sigalova L, Tasdelen B, Oliva J, Barba A. 2007. Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) pesticide multiresidue method in combination with gas and liquid chromatography and tandem mass spectrometric detection. Anal Bioanal Chem 389: 1697-1714.   DOI
13 Sherman M, Ross E, Yates JR 3rd. 1971. Comparative Toxicity of Four Halogenated Organophosphorus Insecticides to Chicks, Japanese Quail, and Diptera. J econ entomol 64: 814-819.   DOI
14 Valverde A, Pihlstrijmb T, Anderssonb A. 1997. Analysis of pyraclofos in crops by the Swedish National Food Administration gas chromatographic multiresidue methodology. Analytica Chimica Acta 338: 63-68.   DOI   ScienceOn