Browse > Article
http://dx.doi.org/10.7853/kjvs.2014.37.2.105

Virucidal efficacy of a disinfectant solution composed of n-alkyl-dimethyl-benzyl-ammonium chloride against porcine epidemic diarrhea virus  

Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
Yu, Eun-Ah (Tongyeong National Quarantine Station, Ministry of Health & Welfare)
Yoo, Chang-Yeul (Department of Computer Information, Gyeongnam Provincial Namhae College)
Cho, Ki-Yung (Meteology Education Platoon, Information & Comunication School, ROKAF Education & Training Command)
Lee, Soo-Ung (Chuncheon Bioindustry Foundation)
Kim, Suk (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University)
Lee, Hu-Jang (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Service / v.37, no.2, 2014 , pp. 105-110 More about this Journal
Abstract
Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED) and causes a considerable economic loss in swine industry. In this study, the virucidal efficacy of the disinfectant composed to n-alkyl-dimethyl-benzyl-ammonium chloride (n-ADBAC) was investigated against PEDV. A virucidal efficacy was determined with the viability of PEDV contacted with the disinfectant in Vero cells. The disinfectant and PEDV were reacted on the hard water (HW) or organic matter suspension (OM) condition. On HW condition, PEDV was inactivated with 50 fold dilutions of the disinfectant. When the antiviral effect on OM condition was evaluated, the antiviral activity of the disinfectant showed on 10 fold dilutions against PEDV. As the disinfectant possesses the virucidal efficacy against PEDV, the disinfectant solution can be used to limit the spread of animal viral diseases.
Keywords
N-alkyl-dimethyl-benzyl-ammonium chloride; Porcine epidemic diarrhea virus; Disinfectant efficacy;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Turgeon DC, Morin M, Jolette J, Higgins R, Marsolais G, DiFranco E. 1980. Coronavirus-like particles associated with diarrhea in baby pigs in Quebec. Can Vet J 21: 100-xxiii.
2 Louati Y, Shaarawy T. 2012. Controversy: Is benzalkonium chloride necessary in antiglaucoma drops? JOCGP 6: 104-107.   DOI
3 Park SJ, Kim HK, Moon HJ, Song DS, Rho SM, Han JY, Nguyen VG, Park BK. 2010. Molecular detection of porcine kobuviruses in pigs in Korea and their association with diarrhea. Arch Virol 155: 1803-1811.   DOI   ScienceOn
4 Pensaert MB, de Bouck P. 1978. A new coronavirus-like particles associated with diarrhea in swine. Arch Virol 58: 243-247.   DOI   ScienceOn
5 Puranaveja S, Poolperm P, Lertwatcharasarakul P, Kesdaengsakonwut S, Boonsoongnern A, Urairong K, Kitikoon P, Choojai P, Kedkovid R, Teankum K, Thanawongnuwech R. 2009. Chinese-like 19 strain of porcine epidemic diarrhea virus, Thailand. Emerg Infect Dis 15: 1112-1115.   DOI
6 Ryan J. 2013. A new threat for swine producers: Porcine epidemic diarrhea virus. Vet Advantage 3: 22-25.
7 Su X, D'Souza DH. 2012. Inactivation of human norovirus surrogates by benzalkonium chloride, potassium peroxymonosulfate, tannic acid, and gallic acid. Foodborne Pathog Dis 9: 829-835.   DOI   ScienceOn
8 The Center for Food Security and Public Health (CFSPH). 2013. Vaccines: Porcine Epidemic Diarrhea. Iowa State University, Ames, IA.
9 Tsao IF, Wang HY, Sipman C. 1989. Interaction of infectious viral particles with a quaternary ammonium chloride (QAC) surface. Biotechnol Bioeng 34: 639-646.   DOI   ScienceOn
10 Tuladhar E, de Koning MC, Fundeanu I, Beumer R, Duizer E. 2012. Different virucidal activities of hyperbranched quaternary ammonium coatings on poliovirus and influenza virus. Appl Environ Microbiol 78: 2456-2458.   DOI
11 Kim WI, Cho HS. 2013. Development of a TaqMan allelic discrimination assay between wild-type and vaccine isolates of porcine epidemic diarrhea virus (PEDV). Philipp J Vet Med 50: 51-54.
12 Duy DT, Toan NT, Puranaveja S, Thanawongnuwech R. 2011. Genetic characterization of porcine epidemic diarrhea virus PEDV isolates from southern Vietnam during 2009-2010 outbreaks. Thai J Vet Med 41: 55-64.
13 Frier M. 1971. Derivatives of 4-Amino-quinaldinium and 8-Hydroxyquinoline. pp. 107-120. In: Hugo WB (ed.). Inhibition and destruction of the microbial cell. Academic Press, London.
14 Karber G. 1931. Beitrag zur kollektiven behandlung pharmakologischer reihenversuche. Arch Exp Pathol Pharmakol 162: 480-487.   DOI
15 Li Z, Chen F, Yuan Y, Zeng X, Wei Z, Zhu L, Sun B, Xie Q, Cao Y, Xue C, Ma J, Bee Y. 2013. Sequence and phylogenetic analysis of nucleocapsid genes of porcine epidemic diarrhea virus (PEDV) strains in China. Arch Virol 158: 1267-1273.   DOI   ScienceOn
16 Debouck P, Pensaert M, Coussement W. 1981. The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, CV 777. Vet Microbiol 6: 157-165.   DOI   ScienceOn
17 Dellanno C, Vega Q, Boesenberg D. 2009. The antiviral action of common household disinfectants and antiseptics against murine hepatitis virus, a potential surrogate for SARS coronavirus. Am J Infect Control 37: 649-652.   DOI   ScienceOn
18 Animal and Plant Quarantine Agency (APQA). 2013. The guideline of the efficacy test for disinfectants. APQA Regulation No. 2013-34, APQA, Anyang.
19 Armstrong JA, Froelich EJ. 1964. Inactivation of viruses by benzalkonium chloride. Appl Microbiol 12: 132-137.
20 Cho WK, Kim H, Choi YJ, Yim NH, Yang HJ, Ma JY. 2012. Epimedium koreanum Nakai water extract exhibits antiviral activity against porcine epidermic diarrhea virus in vitro and in vivo. Evid Based Complement Alternat Med 2012: 985151.
21 Ducatelle R, Coussement W, Pensaert MB. 1981. In vivo morphogenesis of a new porcine enteric coronavirus, CV 777. Arch Virol 68: 35-44.   DOI   ScienceOn
22 Wood EN. 1977. An apparently new syndrome of porcine epidemic diarrhoea. Vet Rec 100: 243-244.   DOI   ScienceOn
23 Pensaert MB. 1999. Porcine epidemic diarrhea virus. pp. 179-185. In: Straw BE, D'Allaire S, Mengeling WL, Taylor DI (ed.). Diseases of Swine. 8th ed. Iowa State University Press, Ames, Iowa.