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http://dx.doi.org/10.7853/kjvs.2011.34.4.397

Application of alkaline phosphatase staining of cytology specimen for differential diagnosis of canine osteosarcoma  

Park, Byoung-Yong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Park, Chul (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Cho, Ho-Seong (College of Veterinary Medicine and Veterinary Diagnostic Center, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.34, no.4, 2011 , pp. 397-401 More about this Journal
Abstract
Aspiration of lytic bone lesions is an excellent diagnostic test in the initial evaluation of primary bone tumor. However, cytologically, it can be difficult to differentiate osteosarcoma (OSA) from other bone neoplasms, including fibrosarcoma, chondrosarcoma, synovial cell sarcoma, malignant fibrous histiocytoma and malignant peripheral nerve sheath tumor. The purpose of this study is to introduce alkaline phosphatase (ALP) staining to differentiate OSA from other mesenchymal tumors. Tumors actively producing bone are specifically positive for ALP staining. Unstained, cytologic specimens were incubated for 10 minutes with nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate toluidine salt-phosphatase substrate. Among 20 cases of cytology specimen, 14 were positive for ALP staining and histopathology, 6 were negative for ALP staining and histopathology. ALP staining was 100% sensitive and specificity for the diagnosis of OSA. Aspirate cytology with ALP staining was a simple, fast, safe and accurate diagnostic test for the evaluation of suspected OSA lesions in dogs.
Keywords
Alkaline phosphatase; Bone; Cytology; Osteosarcoma;
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1 Frostad B, Tani E, Brosjo O, Skoog L, Kogner P. 2002. Fine needle aspiration cytology in the diagnosis and management of children and adolescents with Ewing sarcoma and peripheral primitive neuroectodermal tumor. Med Pediatr Oncol 38: 33-40.   DOI   ScienceOn
2 Jubb KVF, Kennedy PK, Palmer N. 1999. Pathology of domestic animals. 4th ed. pp. 133-135. Academic press, San Diego.
3 Loukopoulos P, Rozmanec M, Sutton RH. 2005. Cytological versus histopathological diagnosis in canine osteosarcoma. Vet Rec 157: 784.
4 Mahaffey EA. 1999. Cytology of the musculoskeletal system. pp. 120-124. In: Cowell RL, Tyler RD, Meinkoth JH. Diagnostic cytology and hematology of the dog and cat. 2nd ed. Mosby, St. Louis, MO.
5 이창우, 조우진, 조재림, 김태승, 황건성. 2001. 골육종의 생화학적 표지자에 관한 연구. 대한골관절종양학회지 7: 41-50.
6 Agarwal S, Agarwal T, Agarwal R, Agarwal PK, Jain UK. 2000. Fine needle aspiration of bone tumors. Cancer Detect Prev 24: 602-609.
7 Brodey RS, Riser WH. 1969. Canine osteosarcoma. A clinicopathologic study of 194 cases. Clin Orthop Relat Res 62: 54-64.
8 Barger A, Graca R, Bailey K, Messick J, de Lorimier LP, Fan T, Hoffmann W. 2005. Use of alkaline phosphatase staining to differentiate canine osteosarcoma from other vimentin-positive tumors. Vet Pathol 42: 161-165.   DOI   ScienceOn
9 Berzina I, Sharkey LC, Matise I, Kramek B. 2008. Correlation between cytologic and histopathologic diagnoses of bone lesions in dogs: a study of the diagnostic accuracy of bone cytology. Vet Clin Pathol 37: 332-338.   DOI   ScienceOn
10 Bleier T, Lewitschek HP, Reinacher M. 2003. Canine osteosarcoma of the penile bone. J Vet Med A Physiol Pathol Clin Med 50: 397-398.   DOI   ScienceOn
11 Shin JB, Park NY, Kim YH, Cho HS. 2007. Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo. Kor J Vet Serv 30: 527-532.
12 Cavalcanti JN, Amstalden EMI, Guerra JL, Magna LC. 2004. Osteosarcoma in dogs: clinical-morphological study and prognostic correlation. Brazil J Vet Res Anim Sci 41: 299-305.
13 Dernell WS, Straw RC, Withrow SJ. 2001. Tumors of the skeletal system. pp. 378-417. In: Withrow SJ, MacEwen EG(ed.). Small animal clinical oncology. 3rd ed. WB Saunders, Philadelphia.
14 Mahaffey EA, Lago MP. 1991. Comparison of techniques for quantifying alkaline phosphatase isoenzymes in canine serum. Vet Clin Pathol 20: 51-55.   DOI   ScienceOn
15 Misdrop W, Hart AA. 1979. Some prognostic and epidemiological factors in canine osteosarcoma. J Natl Cancer Inst 62: 537-545.
16 Phillips L, Hager D, Parker R, Yanik D. 1986. Osteosarcoma with a pathologic fracture in a six month-old dog. Vet Radiol 27: 18-19.   DOI
17 Spodnick GJ, Berg J, Rand WM, Schelling SH, Couto G, Harvey HJ, Henderson RA, MacEwen G, Mauldin N, McCaw DL. Moore AS, Morrison W, Norris AM, O'Bradovich J, O'Keefe DA, Page R, Ruslander D, Klausner J, Straw R, Thompson JP, Withrow SJ. 1992. Prognosis for dogs with appendicular osteosarcoma treated by amputation alone: 162 cases (1978-1988). J Am Vet Med Assoc 200: 995-999.
18 Ward WG Sr, Kilpatrick S. 2000. Fine needle aspiration of primary bone tumors. Clin Orthop Relat Res 373: 80-87.   DOI