[KSCI] Korea Science Citation Index Service

Character of Listeria spp. isolated from livestock products and their related environmental areas

Hur, Jin (College of Veterinary Medicine, Chonbuk National University)
Kim, Jun-Man (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Park, Young-Ho (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)

Publication Information

Korean Journal of Veterinary Service / v.33, no.1, 2010 , pp. 59-66 More about this Journal

Abstract

This study was carried out to investigate the characters of Listeria monocytogenes isolated from food, animal feces, dry cattle food, and the environment in Seoul and Kyonggi province during the period from 1998 to 2003. Serotyping of 70 L. monocytogenes isolates was performed according to the manufacturer's instruction. Minimum inhibitory concentrations (MICs) were determined by the microdilution method according to the guidelines of the Clinical and Laboratory Standards Institute. All the isolates were tested against 20 antimicrobial agents. The serotypes of the 70 L. monocytogenes isolates were 1/2c (62.8%), 1/2a (20%) and 1/2b (17.2%). Of the 70 L. monocytogenes isolates, 67.1%, 57.1%, 11.4%, 5.7%, 2.8%, 1.4% and 1.4% were resistant to tetracycline (Te), minocycline (Mi), norfloxacin (Nor), ciprofloxacin (Cip), neomycin (N), chloramphenicol (C) and cephalothin (Cf), respectively. However, all isolates were 100% sensitive to antibiotics such as amikacin, ampicillin, erythromycin, gentamycin, imipenem, kanamycin, ofloxcin, streptomycin, penicillin, trimethoprim, trimethoprim/sulfamethoxazole, tobramycin, and vancomycin. Multiple resistance patterns of the isolates were observed in TeMiNor Cip (1.4%), TeMiNor (7.1%), TeMiCip (2.9%), TeMiN (1.4%) and TeMi (44.3%). The results of this study indicate that many L. monocytogenes isolates are resistant to antimicrobial agents including Te and Mi. The possibility that the isolates could increasingly acquire multiple antimicrobial resistant properties cannot be precluded.

Keywords

Listeria monocytogenes; Antibiotic susceptibilities; Serotype;

Citations & Related Records

Reference

1	Allerberger F. 2003. Listeria: growth, phenotypic differentiation and molecular micrology. FEMS Immunol Med Microbiol 35: 183-189. DOI ScienceOn
2	Bower CK, Daeschel MA. 1999. Resistance responses of microorganisms in food environments. Int J Food Microbiol 50: 33-44. DOI ScienceOn
3	Poyart-Salmeron C, Carlier C, Trieu-Cuot P, Courtieu AL, Courvalin P. 1990. Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet 335: 1422-1426. DOI ScienceOn
4	Ploy MC, Grlaud C, Martin C, de Lumley L, Denis F. 1998. First clinical isolate of vancomycin-intermediate Staphylococcus aureus in a French hospital. Lancet 351:1212.
5	Quentin C, Thibaut MC, Horovitz J, Bebear C. 1990. Multiresistant strain of Listeria monocytogenes in septic abortion. Lancet 336: 375.
6	Rotun SS, McMath V, Schoonmaker DJ, Maupin PS, Tenover FC, Hill BC, Ackman DA. 1999. Staphylococuus aureus with reduced susceptibility to vancomycin isolated from a patient with fatal bacteremia. Emerg Infect Dis 5: 147-149. DOI ScienceOn
7	Safdar A, Armstrong D. 2003. Antimicrobial activities against 84 Listeria monocytogenes isolates from patients with systemic listeriosis at a comprehensive cancer center (1955-1997). J Clin Microbiol 41: 483-485. DOI ScienceOn
8	Tenover FC, Lancaster MV, Hill BC, Steward CD, Stocker SA, Hancock GA, O'Hara CM, Clark NC, Hiramatsu K. 1998. Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J Clin Microbiol 36: 1020-1027.
9	Salcedo C, Arreaza L, Alcala B, de la Fuente L, Vazquez JA. 2003. Development of a multilocus sequence typing method for analysis of Listeria monocytogenes clones. J Clin Microbiol 41: 757-762. DOI ScienceOn
10	Schuchat A, Swaminathan B, Broome CV. 1991. Epidemiology of human listeriosis. Clin Microbiol Rev 4: 169-183.
11	Tsakaris A, Papa A, Douboyas J, Antoniadis A. 1997. Neonatal meningitidis due to multi-resistant Listeria monocytogenes. J Antimicrob Chemother 39: 553-554. DOI ScienceOn
12	Geornaras I, von Holy A. 2001. Antimicrobial susceptibilities of isolates of Staphylococcus aureus, Listeria species and Salmonella serotypes associated with poultry processing. Int J Food Microbiol 70: 29-35. DOI ScienceOn
13	Gianfranceschi M, Gattuso A, Tartaro S, Aureli P. 2003. Incidence of Listeria monocytogenes in food and environmental samples in Italy between 1990 and 1999: Serotype distribution in food, environmental and clinical samples. Eur J Epidemiol 18: 1001-1006.
14	Hadorn K, Hchler H, Schaffner A, Kayser FH. 1993. Genetic characterization of plasmid-encoded multiple antibiotic resistance in a strain of Listeria monocytogenes causing endocarditis. Eur J Clin Microbiol Infect Dis 12: 928-937. DOI ScienceOn
15	Hof H, Nichterlein T, Kretschmar M. 1997. Management of listeriosis. Clin Microbiol Rev 10: 345-357.
16	Miettinen MK, Siitonen A, Heiskanen P, Haajanen H, Bjrkroth KJ, Korkeala HJ. 1999. Molecular epidemiology of an outbreak of febrile gastroenteritis caused by Listeria monocytogenes in cold-smoked rainbow trout. J Clin Microbiol 37: 2358-2360.
17	Klein G, Pack A, Reuter G. 1998. Antibiotic resistance patterns of enterococci and occurrence of vancomycin resistant enterococci in raw minced beef and pork in Germany. Appl Environ Microbiol 64: 1825-1830.
18	Lukinmaa S, Miettinen M, Nakari UM, Korkeala H, Siitonen A. 2003. Listeria monocytogenes isolates from invasive infection: variation of sero- and- genotypes during an 11-year period in Finland. J Clin Microbiol 41:1694-1700. DOI ScienceOn
19	MacGowan AP, Reeves DS, McLauchlin J. 1990. Antibiotic resistance in Listeria monocytogenes. Lancet 336:513-514.
20	Mulazimoglu L, Drenning SD, Muder RR. 1996. Vancomycin- gentamicin synergism revisited: effect of gentamicin susceptibility of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 40: 1534-1535.
21	Biavasco F, Giovanetti E, Miele A, Vignaroli C, Facinelli B, Varaldo PE. 1996. In vitro conjugative transfer of VanA vancomycin resistance between enterococci and Listeriae of different species. Eur J Clin Microbiol Infect Dis 15: 50-59. DOI ScienceOn
22	Borucki MK, Call DR. 2003. Listeria monocytogenes serotype identification by PCR. J Clin Microbiol 41: 5537-5540. DOI ScienceOn
23	Chasseignaux E, Gerault P, Toquin MT, Salvat G, Colin P, Ermel G. 2002. Ecology of Listeria monocytogenes in the environment of raw poultry meat and raw pork meat processing plants. FEMS Microbiol Lett 210: 271-275. DOI ScienceOn
24	Borucki MK, Krug MJ, Muraoka WT, Call DR. 2003. Discrimination among Listeria monocytogenes isolates using a mixed genome DNA microarray. Vet Microbiol 92:351-362. DOI ScienceOn
25	Charpentier E, Courvalin P. 1999. Antibiotic resistance in Listeria spp. Antimicrob Agents Chemother 43: 2103-2108.
26	Charpentier E, Gerbaud G, Jacquet C, Rocourt J, Courvalin P. 1995. Incidence of antibiotic resistance in Listeria spp. J Infect Dis 172: 277-281. DOI ScienceOn
27	Clinical and Laboratory Standards Institute (CLSI). 2008. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standard, 3rd ed., CLSI document M31-A3. CLSI, Wayne, PA, USA.
28	Facinelli B, Giovanetti E, Varaldo PE, Casolari P, Fabio U. 1991. Antibiotic resistance in foodborne Listeria. Lancet 338: 1272.
29	Farber JM, Peterkin PI. 1991. Listeria monocytogenes, a foodborne pathogen. Microbiol Rev 55: 476-511.
30	Abrahim A, Papa A, Soultos N, Ambrosiadis I, Antoniadis A. 1998. Antibiotic resistance of Salmonella spp. and Listeria spp. isolates from traditionally made fresh sausages in Greece. J Food Pro 61: 1378-1380.
31	Anonymous. 1996. Microbiology of food and animal feeding stuffs-Horizontal method for the detection and enumeration of Listeria monocytogenes. Part 1. Detection method, IOS11290-1.
32	Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Tech Bull Regist Med Technol 36: 49-52.