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Molecular typing of Listeria monocytogenes using pulsed-field gel electrophoresis (PFGE)  

Chae, Hee-Sun (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Kim, Ju-Young (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Kim, Yoen-Ha (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Yang, Yun-Mo (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Jin, Kyong-Sun (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Shin, Bang-Woo (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Lee, Jung-Hark (Seoul Metropolitan Government Research Institute of Public Health & Environment)
Publication Information
Korean Journal of Veterinary Service / v.30, no.3, 2007 , pp. 353-362 More about this Journal
Abstract
A total of 1,354 samples was collected from bovine and porcine carcass from January 2005 to December 2006 in a slaughter house. Twenty five strains(1.8%) of Listeria monocytogenes were isolated from 1,354 samples using selective media. Ten(1.4%) L monocytogenes were isolated from the 677 of bovine carcasses, and 15(2.2%) were isolated from the 677 of porcine carcasses. Among 15 L mono-cytogenes from porcine, 11 siolates were serovars 1/2c, followed by 1/2b (3 strains, 20.0%) and 1/2a(1 strain) Out of 10 bovine samples, positive cases in 1/2a were 9 strains (90.0%), 1/2b were 1 strains(10.0%). PCR primers were selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene (hlyA) of L mono-cytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested. A total of 25 L monocytogenes strains were analysed by PFGE after digestion with Apa I. PFGE analysis of genomic DNA showed the $14{\sim}18$ fragments ranging in size from 30 to 550 kb, resulting in 14 patterns.
Keywords
Listeria monocytogenes; Serovar; PFGE;
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