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Isolation of antibiotic resistant Lactobacillus spp from fermented milk and starters, and detection of transmissible R plasmids  

Baek Kui-Jeong (Jeonbuk Livestock Development & Research Institute)
Seo Heyng-Seok (Jeonbuk Livestock Development & Research Institute)
Roh Young-Sun (Jeonbuk Livestock Development & Research Institute)
Yang Hae-Dong (Statistical Research Institute, Chonbuk National University)
Hur Boo-Hong (Statistical Research Institute, Chonbuk National University)
Seo Lee-Won (Jeonbuk Livestock Development & Research Institute)
Joung Dong-Suk (Jeonbuk Livestock Development & Research Institute)
Song Hee-Jong (Bio-Safety Research Institute)
Publication Information
Korean Journal of Veterinary Service / v.29, no.3, 2006 , pp. 277-285 More about this Journal
Abstract
Plasmids are covalently closed circular molecules of DNA that are stably inherited and replicate somewhat independently of the bacterial chromosome. Genes carried on plasmids can mediate a wide variety of important functions, including antibiotics (R plasmids) and heavy metals resistance, toxins production, cell penetration, iron chelation, complement resistance, and metabolic characteristics such as sucrose and lactose fermentation. Fifty strains of lactobacilli were isolated from 26 staters and 29 fermented milk products. They were classified 27 strains as Lactobacillus paracasei subsp paracasei, 11 stains as Lactococcus lactis subsp cremoris, 6 strains as L delbrueckii subsp lactis, 4 strains as L acidophius, and 2 strains as L delbrueckii subsp bulgaricus. All of these strains were examined for drug resistance and transferability of R plasmids. All of the isolates were sensitive to Am, C, CF, E, NB, P, T, and Te. But resistant to SXT 94% (47 strains), K 66% (33 strains), S 56% (28 strains), ENR 50% (25 strains), NOR 38% (19 strains) CIP 38% (19 strains), GM 16% (8 strains), and N 14% (7 strains), in order. And 32 different resistant patterns were found. The most frequently encountered patterns were CIP-ENR-K-NOR-S-SXT (5 strains). In vitro R plasmids transfer experiment, 57 antibiotic resistant strains which were not transfer to the recipient 2 Escherichia coli strains by conjugation, These results indicate that Lactobacillus in internal trade market' stater recognize R factor but transmissible R plasmid is not existed.
Keywords
Lactobacillus; Starter; Fermented milk; Drug susceptibility; R plasmids;
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