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Comparison of pretreatment methods for tetracyclines analysis by HPLC  

Kim, Dong-Eon (Seoul Metropolitan Health & Environment Research Institute)
Hwang, Lae-Hwong (Seoul Metropolitan Health & Environment Research Institute)
Yun, En-Sun (Seoul Metropolitan Health & Environment Research Institute)
Ham, Hee-Jin (Seoul Metropolitan Health & Environment Research Institute)
Yang, Yoon-Mo (Seoul Metropolitan Health & Environment Research Institute)
Kim, Chang-Gi (Seoul Metropolitan Health & Environment Research Institute)
Ki, No-Jun (Seoul Metropolitan Health & Environment Research Institute)
Lee, Jung-Hark (Seoul Metropolitan Health & Environment Research Institute)
Publication Information
Korean Journal of Veterinary Service / v.26, no.3, 2003 , pp. 199-202 More about this Journal
Abstract
Solid phase extraction(SPE) and matrix solid phase dispersion(MSPD) have been studied as preparation procedures for tetracyclines analysis by high-performance liquid chromatography(HPLC) in meat. The recovery range was 74${\sim}$98% for SPE, and 72${\sim}$93% for MSPD at spiked levels of 100ng/g for oxytetracycline(OTC), tetracycline(TC), chlortetracycline(CTC), and doxycycline(DC). The detection limits were 15${\sim}$78ng/g for SPE and 25${\sim}$84ng/g for MSPD, respectively. Analytical method was HPLC with UV detector. The purpose of this study was developing a practical, accurate and precise method for rapid extraction and quantitation of tetracycline residues in meat.
Keywords
SPE; MSPD; Antibiotics; HPLC;
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  • Reference
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