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Analysis for benzimidazoles in eggs  

Kang, Hyung-Joo (Chungnam Livestock & Veterinary Research Institute)
Kim, Mun-Bae (Chungnam Livestock & Veterinary Research Institute)
Lee, Jae-Bong (Chungnam Livestock & Veterinary Research Institute)
Kim, Bong-Sik (Chungnam Livestock & Veterinary Research Institute)
Kim, Young-Kab (Chungnam Livestock & Veterinary Research Institute)
Publication Information
Korean Journal of Veterinary Service / v.26, no.2, 2003 , pp. 157-162 More about this Journal
Abstract
This stud was attempted to detect six benzimidazoles[thiabendazole(TBZ), oxfendazole(OFZ), mebendazole(MBZ), flubendazole(FLBZ), albendazole(ABZ), and febendazole(FBZ)] in eggs using high performance liquid chromatography(HPLC) with photodiode array detector(DAD) simultaneously. The eluates were determined by HPLC on a waters X-Terra$^{TM}\;C_{18}$ reverse-phase column($4.6{\times}250nm,\;5{\mu}m$) with DAD at 295nm. The mobile phase was 0.04M ammonium phosphate(pH 7.5)/ACN(62.28 v/v) run isocratically. The calibration curves were linear(r>0.999) for six benzimidazoles. Average recovery rate from eggs were 94.54% for TBZ, 97.71% for OFZ, 88.82% for MBZ, 93.71% for FLBZ, 78.61% for ABZ, and 75.09% for FBZ, respectively. The limits of detection and quantification were 2.27ppb, 3.88 for TBZ, 9.34ppb, 15.61ppb for OFZ, 28.53ppb, 45.15ppb for MBZ, 27.39ppb, 40.95ppb for FBZ, 8.61ppb, 13.95ppb for ABZ and 12.79ppb, 22.34ppb for FBZ, respectively.
Keywords
Benzimidazoles; Eggs; HPLC; DAD;
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