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Study on analytical method of residual macrolide antibiotics in livestock products by LC/MS  

황래홍 (서울특별시보건환경연구원)
윤은선 (서울특별시보건환경연구원)
김연주 (서울특별시보건환경연구원)
김동언 (서울특별시보건환경연구원)
양윤모 (서울특별시보건환경연구원)
이정학 (서울특별시보건환경연구원)
이병동 (서울특별시보건환경연구원)
Publication Information
Korean Journal of Veterinary Service / v.25, no.3, 2002 , pp. 221-227 More about this Journal
Abstract
This study was carried out to confirm analytical method of residual macrolides in livestock products by LC/MS. 1. Macrolides were analyzed by LC/MS on XTerra C$\sub$18/ column with 0.1% TFA(trifluoroacetic acid)-methanol in a gradient mode as mobile phase, and that were identified by positive chemical ionization with selective ion monitoring at 50～1000 mass range. 2. Residual macrolides were extracted from tissue with acetonitrile, and the extract is purified with a Sep-pak C$\sub$18/ cartridge, and elute macrolides with 0.1M methanolic ammonium acetate. 3. The procedure confirms the presence of each macrolide at 50$\mu\textrm{g}$/kg in spiked sample.
Keywords
Macrolide antibiotics; LC/MS; Livestock products;
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