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http://dx.doi.org/10.13160/ricns.2018.11.2.69

Extracts from the Red Algae Gracilaria vermiculophylla have Antioxidant Effects in Human Bone Marrow Mesenchymal Stem Cells  

Jeong, Sin-Gu (Department of Biology, College of Natural Science, Chosun University)
Lee, Jae-Joon (Department of Food and Nutrition, College of Natural Science, Chosun University)
Kim, Ho-Tae (Department of Biology, College of Natural Science, Chosun University)
Ahn, Min-Ji (Department of Biology, College of Natural Science, Chosun University)
Son, Hee-Kyoung (Department of Food and Nutrition, College of Natural Science, Chosun University)
Lee, Jun Sik (Department of Biology, College of Natural Science, Chosun University)
Oh, Won Keun (Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Science, College of Pharmacy, Seoul National University)
Cho, Tae Oh (Department of Biology, College of Natural Science, Chosun University)
Cho, Goang-Won (Department of Biology, College of Natural Science, Chosun University)
Publication Information
Journal of Integrative Natural Science / v.11, no.2, 2018 , pp. 69-75 More about this Journal
Abstract
The red algae Gracilaria vermiculophylla is widely spread around seaside areas across the globe, and has been used as a food resource in Southeast Asian countries. Previous studies have shown that Gracilaria red algae extracts have beneficial antihypercholesterolemic, antioxidant, anti-inflammatory, and antimicrobial effects. In this study, we investigated the antioxidant effects of Gracilaria vermiculophylla extracts (GV-Ex) on human bone marrow mesenchymal stem cells (hBM-MSCs). The acetone and DMSO/ethanol solvents of the tested GV contain higher total flavonoid and polyphenolic contents that can strongly scavenge reactive oxygen species (ROS). Pre-treatment with GV-Ex protected hBM-MSCs against oxidative stress induced by hydrogen peroxide treatment. The protective effects of GV-Ex treatment were confirmed by MTT assay. The elevated levels of ROS in hBM-MSCs caused by hydrogen peroxide induced oxidative stress were significantly decreased by GV extract treatment. The levels of the antioxidant proteins superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase (CAT) were also restored or protected by GV-Ex treatment, suggesting that GV extracts moderate excess ROS levels and prevent cells from oxidative damage.
Keywords
Gracilaria vermiculophylla; Anti-oxidant; Mesenchymal Stem Cells; Reactive Oxygen Species; ROS;
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