Browse > Article
http://dx.doi.org/10.13160/ricns.2018.11.2.101

Protein-Protein Interaction Analysis of Corticotropin - Releasing Hormone Receptor 1 with Corticotropin-Releasing Hormone and Sauvagine  

Nagarajan, Santhosh Kumar (Department of Genetic Engineering, School of Bioengineering, SRM University)
Publication Information
Journal of Integrative Natural Science / v.11, no.2, 2018 , pp. 101-106 More about this Journal
Abstract
Corticotropin - releasing hormone receptor 1 (CRHR1) forms an integral part of the pathophysiology of disorders like post-traumatic stress disorder, stress, anxiety, addiction, and depression. Hence it is essential to look for new, potent and structure-specific inhibitors of CRHR1. We have analysed the protein-protein interaction complexes of the CRHR1 receptor with its native ligand CRF and full agonist Sauvagine. The structure of Sauvagine was predicted using homology modelling. We have identified that the residues TYR253, ASP254, GLU256, GLY265, ARG1014 and LY1060 are important in the formation of protein-protein complex formation. Future studies on these residues could throw light on the crucial structural features required for the formation of CRHR1-inhibitor complex and in studies that try to solve the structural complexities of CRHR1.
Keywords
Corticotropin - Releasing Hormone Receptor 1; CRHR1; CRF; Sauvagine; Protein-Protein Docking;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 B. P. Grone and K. P. Maruska, "A second corticotropin-releasing hormone gene (CRH2) is conserved across vertebrate classes and expressed in the hindbrain of a basal neopterygian fish, the spotted gar", J. Comp. Neurol., Vol. 523, pp. 2015.
2 R. L. Hauger, D. E. Grigoriadis, M. F. Dallman, P. M. Plotsky, W. W. Vale, and F. M. Dautzenberg, "Current status of the nomenclature for receptors for corticotropin-releasing factor and their ligands", Pharmacol. Rev., Vol. 55, pp. 21-26, 2003.   DOI
3 N. Sato, K. Takagi, T. Suzuki, Y. Miki, S. Tanaka, S. Nagase, H. Warita, S. Fukudo, F. Sato, H. Sasano, and K. Ito, "Immunolocalization of corti-cotropin-releasing hormone (CRH) and its receptors (CRHR1 and CRHR2) in human endometrial car-cinoma: CRHR1 as a potent prognostic factor", Int. J. Gynecol. Cancer, Vol. 24, pp. 1549-1557, 2014.   DOI
4 F. Stamatelou, E. Deligeoroglou, N. Vrachnis, S. Iliodromiti, Z. Iliodromiti, S. Sifakis, G. Far-makides, and G. Creatsas, "Corticotropin-releasing hormone and progesterone plasma levels association with the onset and progression of labor", Clin. Exp. Obstet. Gynecol., 2013
5 F. C. Raadsheer, J. J. van Heerikhuize, P. J. Lucas- sen, and W. J. Hoogendijk, "Corticotropin-releasing hormone mRNA levels in the paraventricular nucleus of patients with Alzheimer's disease and depression", Am. J. Psychiatry, Vol. 152, pp. 1372-1376, 1995.   DOI
6 H.-B. Fan, T. Zhang, K. Sun, S.-P. Song, S.-B. Cao, H.-L. Zhang, and W. Shen, "Corticotropin-releasing factor mediates bone cancer induced pain through neuronal activation in rat spinal cord", Tumor Bilogy, Vol. 36, pp. 9559-9565, 2015.   DOI
7 R. Pastor, C. S. McKinnon, A. C. Scibelli, S. Burkhart-Kasch, C. Reed, A. E. Ryabinin, S. C. Coste, M. P. Stenzel-Poore, and T. J. Phillips, "Corticotropin-releasing factor-1 receptor involvement in behavioral neuroadaptation to ethanol: A urocortin1-independent mechanism", Proc. Natl. Acad. Sci. U.S.A., Vol. 105, pp. 9070-9075, 2008.   DOI
8 V. Coric, H. H. Feldman, D. A. Oren, A. Shekhar, J. Pultz, R. C. Dockens, X. Wu, K. A. Gentile, S.?P. Huang, E. Emison, T. Delmonte, B. B. D'Souza, D. L. Zimbroff, J. A. Grebb, A. W. Goddard, and E. G. Stock, "Multicenter, randomized, double-blind, active comparator and placebo-controlled trial of a corticotropin-releasing factor receptor-1 antagonist in generalized anxiety disorder", Depress. Anxiety, Vol. 27, pp. 417-425, 2010   DOI
9 E. M. Jutkiewicz, S. K. Wood, and J. H. Woods, "The effects of CRF antagonists, antalarmin, CP154,526, LWH234, and R121919, in the forced swim test and on swim-induced increases in adre- nocorticotropin in rats", Psychopharmacology (Berl.), Vol. 180, pp. 215-223, 2005.   DOI
10 B. Binneman, D. Feltner, S. Kolluri, Y. Shi, R. Qiu, and T. Stiger, "A 6-week randomized, placebo-con- trolled trial of CP-316,311 (a selective CRH1 antag- onist) in the treatment of major depression", Am. J. Psychiatry, Vol. 165, pp. 617-620, 2008.   DOI
11 M. Wiederstein and M. J. Sippl, "ProSA-web: inter- active web service for the recognition of errors in three-dimensional structures of proteins", Nucleic Acids Res., Vol. 35, pp. W407-W410. 2007.   DOI
12 S. K. Nagarajan and T. Madhavan, "3D-QSAR studies of 8-substituted-2-aryl-5-alkylaminoquino-lines as corticotropin-releasing factor-1 receptor antagonists", J. Chosun Natural Sci., Vol. 8, pp. 176-183, 2015.   DOI
13 (a) S. K. Nagarajan, T. Madhavan, "Comparative molecular similarity indices analysis (CoMSIA) of 8-substituted-2-aryl-5-alkylaminoquinolines as corticotropin-releasing factor-1 receptor antagonists", J. Chosun Natural Sci., Vol. 9, pp. 241-248, 2016.   DOI
14 Y. Shen, J. Maupetit, P. Derreumaux, and P. Tufféry, "Improved PEP-FOLD approach for peptide and miniprotein structure prediction", J. Chem. Theory Comput., Vol. 10, pp. 4745-4758. 2014.   DOI
15 S. C. Lovell, I. W. Davis, W. B. Arendall III, P. I. W. de Bakker, J. M. Word, M. G. Prisant, J. S. Richardson, and D. C. Richardson, "Structure validation by Ca geometry: f, y and Cb deviation", Proteins, Vol. 50, pp. 437-450, 2003.   DOI
16 C. Colovos and T. O. Yeates," Verification of protein structures: patterns of nonbonded atomic inter-actions", Protein Sci., Vol. 2, pp. 1511-1519. 1993.   DOI
17 S. R. Comeau, D. W. Gatchell, S. Vajda, and C. Camacho, "ClusPro: an automated docking and dis- crimination method for the prediction of protein complexes", Bioinformatics, Vol. 20, pp. 45-50. 2003.
18 S. C. Comeau, D. W. Gatchell, S. Vajda, and C. Camacho, "ClusPro: a fully automated algorithm for protein-protein docking", Nucleic Acids Res, Vol. 32, pp. 96-99, 2004.
19 D. Kozakov, D. Beglov, T. Bohnuud, S. Mottarella, B. Xia, D. R. Hall, and S. Vajda, "How good is automated protein docking?", Proteins, Vol. 81, pp. 2159-2166, 2013.   DOI
20 M. F. Lensink and S. J. Wodak, "Docking, scoring, and affinity prediction in CAPRI", Proteins, Vol. 81, pp. 2082-2095. 2013.   DOI
21 D. Kozakov, R. Brenke, S. R. Comeau, and S. Vajda, "PIPER: An FFT-based protein docking program with pairwise potentials", Proteins, Vol. 65, pp. 392-406, 2006.   DOI
22 S. K. Nagarajan, "Protein-Protein Interaction Analysis of KiSS1-Derived Peptide Receptor with Kisspeptin-10 and Kisspeptin-15", J. Chosun Natural Sci., Vol. 11, pp. 9-13, 2018.
23 B. Sathya, "Hologram Based QSAR Analysis of Xanthine Oxidase Inhibitors", J. Chosun Natural Sci., Vol. 10, pp. 202-208, 2017.