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http://dx.doi.org/10.7586/jkbns.2020.22.4.223

Effects of Taro Extract on Brain Resilience in In Vitro Parkinson's Disease Model Induced by 6-Hydroxydopamine  

Cho, Hyeyoung (Department of Nursing, Kunsan National University)
Kang, Kyoungah (Department of Nursing, Kunsan National University)
Publication Information
Journal of Korean Biological Nursing Science / v.22, no.4, 2020 , pp. 223-231 More about this Journal
Abstract
Purpose: The purpose of this study was to investigate the effects of taro extract on brain resilience in in vitro Parkinson's disease model induced by 6-hydroxydopamine (6-OHDA). Methods: To induce a neuroinflammatory reaction and the in vitro Parkinson's disease model, SH-SY5Y cells were stimulated with lipopolysaccharide (LPS) and 6-OHDA, respectively. After that, cells were treated with at various concentrations (1, 5, and 10 mg/mL) of taro extract. Then nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), interleukin (IL)-6, synaptophysin (SYP) and growth associated protein (GAP)-43 messenger ribonucleic acid (mRNA) expression level were measured. Results: Taro extract significantly suppressed LPS-induced NO production. Meanwhile, iNOS and IL-6 mRNA expression decreased in a dose-dependent manner. In addition, taro increased the mRNA expression of SYP and GAP-43 mRNA. Conclusion: These findings indicate that taro played an important role in brain resilience by inhibiting neuronal cell death and promoting neurite outgrowth, synaptogenesis, and neural plasticity. The results of this study suggest that taro may contribute to the prevention of neurodegenerative disease and become a new and safe therapeutic strategy for Parkinson's disease.
Keywords
Neuroprotection; Parkinson disease; Resilience; Taro;
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1 Nithianantharajah J, Hannan AJ. Enriched environments, experience dependent plasticity and disorders of the nervous system. Nature Reviews Neuroscience. 2006;7(9):697-709. https://doi.org/10.1038/nrn1970   DOI
2 Holahan M. GAP-43 in synaptic plasticity: molecular perspectives. Research and Reports in Biochemistry. 2015;5:137-146. https://doi.org/10.2147/RRBC.S73846   DOI
3 Kalia LV, Lang AE. Parkinson's disease. Lancet. 2015;386(9996):896-912. https://doi.org/10.1016/s0140-6736(14)61393-3   DOI
4 Weerkamp NJ, Tissingh G, Poels PJ, Zuidema SU, Munneke M, Koopmans RT, et al. Nonmotor symptoms in nursing home residents with Parkinson's disease: prevalence and effect on quality of life. Journal of the American Geriatrics Society. 2013;61(10):1714-1721. https://doi.org/10.1111/jgs.12458   DOI
5 Lee JE, Choi JK, Lim HS, Kim JH, Cho JH, Kim GS, et al. The prevalence and incidence of parkinson's disease in south korea: a 10-year nationwide population-based study. Journal of the Korean Neurological Association. 2017;35(4):191-198. https://doi.org/10.17340/jkna.2017.4.1.   DOI
6 Fried TR, O'Leary J, Towle V, Goldstein MK, Trentalange M, Martin DK. Health outcomes associated with polypharmacy in community-dwelling older adults: a systematic review. Journal of the American Geriatrics Society. 2014;62(12):2261-2272. https://doi.org/10.1111/jgs.13153   DOI
7 Park HY, Park JW, Sohn HS, Kwon JW. Association of parkinsonism or parkinson disease with polypharmacy in the year preceding diagnosis: a nested casecontrol study in south korea. Drug Safety. 2017;40(11):1109-1118. https://doi.org/10.1007/s40264-017-0559-5   DOI
8 Walrand S. Dietary supplement intake among the elderly: hazards and benefits. Current Opinion in Clinical Nutrition and Metabolic Care. 2018;21(6):465-470. https://doi.org/10.1097/mco.0000000000000512   DOI
9 Jeon YH, Lee JW, Son YJ, Hwang IK. Characteristics and sensory optimization of taro (Colocasia esculenta) under different aging conditions for food application of black taro. Korean Journal of Food Science and Technology. 2016;48(2):133-141. https://doi.org/10.9721/KJFST.2016.48.2.133   DOI
10 Kalariya M, Prajapati R, Parmar SK, Sheth N. Effect of hydroalcoholic extract of leaves of colocasia esculenta on marble-burying behavior in mice: Implications for obsessive-compulsive disorder. Pharmaceutical Biology. 2015;53(8):1239-1242. https://doi.org/10.3109/13880209.2015.1014923   DOI
11 Park GH, Kim HG, Ju MS, Kim AJ, Oh MS. Thuja orientalis leaves extract protects dopaminergic neurons against MPTP-induced neurotoxicity via inhibiting inflammatory action. The Korea Journal of Herbology. 2014;29(3):27-33. http://dx.doi.org/10.6116/kjh.2014.29.3.27.   DOI
12 Lee JS, Lee JY, Cho WG, Yang YC, Cho BP. Relationship between microglial activation and dopaminergic neuronal loss in 6-OHDA-induced parkinsonian animal model. Korean Journal of Physical Anthropologists. 2013;26(1):13-23. http://dx.doi.org/10.11637/kjpa.2013.26.1.13   DOI
13 Lee C, Jang JH, Park GH. Protective effect of korean red ginseng against 6-hydroxydopamine-induced nitrosative cell death via fortifying cellular defense system. Yakhak Hoeji. 2016;60(2):92-99. http://dx.doi.org/10.17480/psk.2016.60.2.92   DOI
14 Kaushal P, Kumar V, Sharma HK. Utilization of taro (Colocasia esculenta): a review. Journal of Food Science and Technology. 2013;52(1):27-40. https://doi.org/10.1007/s13197-013-0933-y   DOI
15 Awa E, Eleazu C. Bioactive constituents and antioxidant activities of raw and processed cocoyam (Colocasia esculenta). Nutrafoods. 2015;14(3):133-140. https://doi.org/10.1007/s13749-015-0033-x   DOI
16 Rappold PM, Tieu K. Astrocytes and therapeutics for Parkinson's disease. Neurotherapeutics. 2010;7(4):413-423. https://doi.org/10.1016/j.nurt.2010.07.001   DOI
17 Xie HR, Hu LS, Li GY. SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in parkinson's disease. Chinese Medical Journal. 2010;123(8):1086-1092. https://doi.org/10.3760/cma.j.issn.0366-6999.2010.08.021   DOI
18 Rivetti di Val Cervo P, Romanov RA, Spigolon G, Masini D, Martin-Montanez E, Toledo EM, et al. Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model. Nature Biotechnology. 2017;35(5):444-452. https://doi.org/10.1038/nbt.3835   DOI
19 Heneka MT, Feinstein DL. Expression and function of inducible nitric oxide synthase in neurons. Journal of Neuroimmunology. 2001;114(1-2):8-18. https://doi.org/10.1016/S0165-5728(01)00246-6   DOI
20 Barthwal MK, Srivastava N, Dikshit M. Role of nitric oxide in a progressive neurodegeneration model of parkinson's disease in the rat. Redox Report. 2001;6(5):297-302. https://doi.org/10.1179/135100001101536436   DOI
21 Gao Z, Ure K, Ables JL, Lagace DC, Nave KA, Goebbels S, et al. Neurod-1 is essential for the survival and maturation of adult-born neurons. Nature Neuroscience. 2009;12(9):1090-1092. https://doi.org/10.1038/nn.2385   DOI
22 Guo Z, Zhang L, Wu Z, Chen Y, Wang F, Chen G. In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer's disease model. Cell Stem Cell. 2014;14(2):188-202. https://doi.org/10.1016/jstem.2013.12.001   DOI
23 Ahn SH, Chang IA, Kim KJ, Kim CJ, Namgung UK, Cho CS. Bogijetong decoction and its active herbal components protect the peripheral nerve from damage caused by taxol or nerve crush. BMC Complementary and Alternative Medicine. 2016;16:402. https://doi.org/10.1186/s12906-016-1391-7   DOI
24 Burke RE, O'Malley K. Axon degeneration in parkinson's disease. Experimental Neurology. 2013;246:72-83. https://doi.org/10.1016/j.expneurol.2012.01.011   DOI
25 Schmitt U, Tanimoto N, Seeliger M, Schaeffel F, Leube RE. Detection of behavioral alterations and learning deficits in mice lacking synaptophysin. Neuroscience. 2009;162(2):234-243. https://doi.org/10.1016/j.neuroscience.2009.04.046   DOI
26 Zhu X, Wang P, Liu H, Zhan J, Wang J, Li M, Zeng L, Xu P. Changes and significance of SYP and GAP-43 expression in the hippocampus of CIH Rats. International Journal of Medical Science. 2019;16(3):394-402. https://doi.org/10.7150/ijms.28359   DOI
27 Shin MS, Jeong HY, An DI, Lee HY, Sung YH. Treadmill exercise facilitates synaptic plasticity on dopaminergic neurons and fibers in the mouse model with Parkinson's disease. Neuroscience Letters. 2016;621:28-33. https://doi.org/10.1016/j.neulet.2016.04.015   DOI
28 Rakic S, Hung Y, Smith M, So D, Tayler HM, Varney W, et al. Systemic infection modifies the neuroinflammatory response in late stage alzheimer's disease. Acta Neuropathologica Communications. 2018;6(1):88. https://doi.org/10.1186/s40478-018-0592-3   DOI
29 Cong Q, Soteros BM, Wollet M. Kim JH, Sia GM. The endogenous neuronal complement inhibitor SRPX2 protects against complement-mediated synapse elimination during development. Nature Neuroscience. 2020. Forthcoming. https://doi.org/10.1038/s41593-020-0672-0   DOI
30 Kim HS, Kim MK, Lee M, Kwon BS, Suh DH, Song YS. Effect of red ginseng on genotoxicity and health-related quality of life after adjuvant chemotherapy in patients with epithelial ovarian cancer: a randomized, double blind, placebocontrolled trial. Nutrients. 2017;9(7):772. https://doi.org/10.3390/nu9070772   DOI
31 Lu X, Kim-Han JS, Harmon S, Sakiyama-Elbert SE, O'Malley KL. The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons. Molecular Neurodegeneration. 2014;9:17. https://doi.org/10.1186/1750-1326-9-17   DOI
32 Li H, Dong Z, Liu X, Chen H, Lai F, Zhang M. Structure characterization of two novel polysaccharides from colocasia esculenta (taro) and a comparative study of their immunomodulatory activities. Journal of Functional Foods. 2018;42:47-57. https://doi.org/10.1016/j.jff.2017.12.067   DOI
33 Moon JH, Sung JH, Choi IW, Kim YS. Anti-obesity and hypolipidemic activity of taro powder in mice fed with high fat and cholesterol diets. Korean Journal of Food Science and Technology. 2010;42(5):620-626.
34 Pereira PR, Correa ACNTF, Vericimo MF, Paschoalin VMF. Tarin, a potential immuno-modulator and COX Inhibitor lectin found in taro (Colocasia esculenta). Comprehensive Reviews in Food Science and Food Safety. 2018;17(4): 878-891. https://doi.org/10.1111/1541-4337.12358   DOI
35 Brown GC, Neher JJ. Inflammatory neurodegeneration and mechanisms of microglial killing of neurons. Molecular Neurobiology. 2010;41(2-3):242-247. https://doi.org/10.1007/s12035-010-8105-9   DOI
36 Volosin M, Song W, Almeida RD, Kaplan DR, Hempstead BL, Friedman WJ. Interaction of survival and death signaling in basal forebrain neurons: roles of neurotrophins and proneurotrophins. The Journal of Neuroscience. 2006;26(29):7756-7766. https://doi.org/10.1523/jneurosci.1560-06.2006   DOI