Browse > Article
http://dx.doi.org/10.5333/KGFS.2021.41.3.210

Citric Acid Reduces Alkaline Stress-induced Chlorosis, Oxidative Stress, and Photosynthetic Disturbance by Regulating Growth Performance, Antioxidant Activity and ROS Scavenging in Alfalfa  

Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Song, Yowook (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Woo, Jae Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Choi, Bo Ram (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Lim, Eun A (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Rahman, Md Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.41, no.3, 2021 , pp. 210-216 More about this Journal
Abstract
Pollution of agricultural soil by alkaline salts, such as Na2CO3, is a critical and long-lasting problem in cultivable land. The aim of the study was to examine the putative role of citric acid (CA) in alleviating Na2CO3-stress in alfalfa. In this study, Na2CO3 significantly induced leaf chlorosis, inhibited plant growth and photosynthesis related parameters, increased hydrogen peroxide (H2O2) and reduced major antioxidant enzymes (SOD, CAD, APX) in alfalfa. However, the presence of CA these negative effects of Na2CO3-stress largely recovered. Interestingly, expression of antioxidant and ion transporter genes (Fe-SOD, CAT, APX, DHAR and NHX1) involved in Reactive oxygen species (ROS) homeostasis and oxidative stress tolerance in alfalfa. These findings suggest that CA-mediated Na2CO3 stress alleviation is an ecofriendly approach that would be useful to local farmer for alfalfa and other forage crop cultivation in alkaline soils.
Keywords
Citric acid; alkaline; Photosynthetic disturbance; ROS; Alfalfa;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Irakoze, W., Prodjinoto, H., Nijimbere, S., Bizimana, J.B., Bigirimana, J., Rufyikiri, G. and Lutts, S. 2021. NaCl- and Na2SO4-induced salinity differentially affect clay soil chemical properties and yield components of two rice cultivars (Oryza sativa L.) in Burundi. Agronomy. 11(3):571.   DOI
2 Kabir, A.H., Das, U., Rahman, M.A. and Lee, K.W. 2021. Silicon induces metallochaperone-driven cadmium binding to the cell wall and restores redox status through elevated glutathione in Cd-stressed sugar beet. Physiologia Plantarum. 173(1):352-368.
3 Kulkarni, K.P., Tayade, R., Asekova, S., Song, J.T., Shannon, J.G. and Lee, J.D. 2018. Harnessing the potential of forage legumes, alfalfa, soybean, and cowpea for sustainable agriculture and global food security. Frontiers of Plant Science. 9:1314.   DOI
4 Li, R., Shi, F., Fukuda, K. and Yang, Y. 2010. Effects of salt and alkali stresses on germination, growth, photosynthesis and ion accumulation in alfalfa (Medicago sativa L.). Soil Science and Plant Nutrition. 56(5):725-733.   DOI
5 Livak, K.J. and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods. 25:402-408.   DOI
6 Paz, R.C., Rocco, R.A., Reinoso, H., Menendez, A.B., Pieckenstain, F.L. and Ruiz, O.A. 2012. Comparative study of alkaline, saline, and mixed saline-alkaline stresses with regard to their effects on growth, nutrient accumulation, and root morphology of Lotus tenuis. Journal of Plant Growth Regulation. 31(3):448-459.   DOI
7 Haque, A.F.M.M., Tasnim, J., El-Shehawi, A.M., Rahman, M.A., Parvez, M.S., Ahmed, M.B. and Kabir, A.H. 2021. The Cd-induced morphological and photosynthetic disruption is related to the reduced Fe status and increased oxidative injuries in sugar beet. Plant Physiology and Biochemistry. 166:448-458.   DOI
8 Assaha, D.V.M., Ueda, A., Saneoka, H., Al-Yahyai, R. and Yaish, M.W. 2017. The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Frontiers of Plant Science. 8:509.   DOI
9 Fang, S., Hou, X. and Liang, X. 2021. Response mechanisms of plants under saline-alkali stress. Frontiers of Plant Science. 12:1049.
10 Guo, R., Shi, L., Yan, C., Zhong, X., Gu, F., Liu, Q., Xia, X. and Li, H. 2017. Ionomic and metabolic responses to neutral salt or alkaline salt stresses in maize (Zea mays L.) seedlings. BMC Plant Biology. 17(1):41.   DOI
11 Hoagland, D.R. and Arnon, D.I. 1950. The water-culture method for growing plants without soil. California Agricultural Experiment Station. 347:32.
12 Pilon, M., Ravet, K. and Tapken, W. 2011. The biogenesis and physiological function of chloroplast superoxide dismutases. Biochimica and Biophysica Acta (BBA)- Bioenergetics. 1807:989-998.   DOI
13 Rahman, M.A., Alam, I., Kim, Y.G., Ahn, N.Y., Heo, S.H., Lee, D.G., Liu, G. and Lee, B.H. 2015. Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach. Plant Physiology and Biochemistry. 89:112-122.   DOI
14 Sun, Y.L. and Hong, S.K. 2011. Effects of citric acid as an important component of the responses to saline and alkaline stress in the halophyte Leymus chinensis (Trin.). Plant Growth Regulation. 64(2):129-139.   DOI
15 Peng, Y.L., Gao, Z.W., Gao, Y., Liu, G.F., Sheng, L.X. and Wang, D.L. 2008. Eco-physiological characteristics of alfalfa seedlings in response to various mixed salt-alkaline stresses. Journal of Integrative Plant Biology. 50(1):29-39.   DOI
16 Rahman, M.A., Kim, Y.G., Alam, I., Liu, G., Lee, H., Lee, J.J. and Lee, B.H. 2016. Proteome analysis of alfalfa roots in response to water deficit stress. Journal of Integrative Agriculture. 15(6):1275-1285.   DOI
17 Rahman, M.A., Parvin, M., Das, U., Ela, E.J., Lee, S.H., Lee, K.W. and Kabir, A.H. 2020. Arbuscular mycorrhizal symbiosis mitigates iron (Fe)-deficiency retardation in alfalfa (Medicago sativa L.) through the enhancement of Fe accumulation and sulfur-assisted antioxidant defense. International Journal of Molecular Science. 21:2219.   DOI
18 Zhang, J.T. and Mu, C.S. 2009. Effects of saline and alkaline stresses on the germination, growth, photosynthesis, ionic balance and anti-oxidant system in an alkali-tolerant leguminous forage Lathyrus quinquenervius. Soil Science and Plant Nutrition. 55(5):685-697.   DOI
19 Tahjib-Ul-Arif, M., Zahan, M.I., Karim, M.M., Imran, S., Hunter, C.T., Islam, M.S., Mia, M.A., Hannan, M.A., Rhaman, M.S., Hossain, M.A., Brestic, M., Skalicky, M. and Murata, Y. 2021. Citric acid-mediated abiotic stress tolerance in plants. International Journal of Molecular Sciences. 22(13):7235.   DOI
20 Wu, Z.H., Yang, C.W. and Yang, M.Y. 2014. Photosynthesis, photosystem II efficiency, amino acid metabolism and ion distribution in rice (Oryza sativa L.) in response to alkaline stress. Photosynthetica. 52:157-160.   DOI
21 Hassani, A., Azapagic, A. and Shokri, N. 2020. Predicting long-term dynamics of soil salinity and sodicity on a global scale. Proceedings of the National Academy of Sciences of the United State of America. 117(52):33017-33027.   DOI
22 Funck, D., Baumgarten, L., Stift, M., Von Wiren, N. and Schonemann, L. 2020. Differential contribution of P5CS isoforms to stress tolerance in Arabidopsis. Frontiers in Plant Science. 11:1483.