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http://dx.doi.org/10.5333/KGFS.2020.40.2.80

The Effect of Ammonia and Sodium Hydroxide Treatment on the Storage and Rumen Microbial Fiber Degradation in Silage of Rice Straw Contaminated Mycotoxin

Sung, Ha Guyn (Dept. of Animal Science, Sangji University)

Publication Information

Journal of The Korean Society of Grassland and Forage Science / v.40, no.2, 2020 , pp. 80-86 More about this Journal

Abstract

This study was conducted to research on the efficacy of chemical treatment as an effective method for reducing mycotoxin in rice straw silage. As a chemical treatment method, ammonia and sodium hydroxid were treated at 4% level of rice straws contaminated with mycotoxin, and the effects of silage storage on fungal toxin reduction, fermentation quality, and fiber digestion were evaluated. Aflatoxin B₁, B₂, G₁, G₂ and fumonisin B₁, B₂ as well as deoxynivalenol were not detected in all experimental groups, and ochratoxin A and zearalenone were detected. Ochratoxin A was detected lower in the chemical treatment than control (41.23 g / kg) (p<0.05). Zearalenone showed lower results in sodium hydroxide treatment (297.44 ㎍ / kg) than control (600.33 ㎍ / kg) and ammonia treatment (376.00 ㎍ / kg) (p<0.05). The pH of rice straw silage was the lowest in ammonia treatment and the highest in sodium hydroxide treatment (p<0.05). The lactic acid contents of control and ammonia treatments were similar, but sodium hydroxide treatment was the lowest (p<0.05). Propionic acid was higher in the control than in the chemical treatments (p<0.05), and showed similar contents in the ammonia and sodium hydroxide treatment. Both the rumen microbial degradation rate of NDF and ADF showed the highest in sodium hydroxide treatment, followed by ammonia treatment, and the control showed the lowest level (p<0.05). Therefore, the results of this study are demonstrated to have a good effect on the treatment of ammonia and sodium hydroxide to reduce the mycotoxins and increase the rumen microbial degradation rate in the rice straw silage. Sodium hydroxide treatment was more effective in reducing mycotoxins and improving fiber degradation rate than ammonia treatment, but it is thought to have an inefficient effect on silage fermentation in rice straw silage.

본 연구는 볏짚 사일리지의 곰팡이 독소 저감을 위한 효율적 방안으로 화학적 처리 방법에 따른 효능에 관한 진보적 연구 자료를 제공하고자 실시하였다. 화학적 처리 방법으로 암모니아 및 가성소다를 이용 곰팡이독소에 오염된 볏짚의 4% 수준으로 각각 처리하고 사일리지 저장에 따른 곰팡이독소 저감, 발효 품질 및 섬유소 소화에 미치는 영향을 평가 하였다. 모든 실험구에서 Aflatoxin B₁, B₂, G₁, G₂ 그리고 Fomonisin B₁, B₂ 뿐만 아니라 Dexynivalenol은 검출되지 않았으며, Ochratoxin A와 Zearalenone이 검출되었다. 그리고 Ochratoxin A은 대조구(41.23g/kg)보다 화학적 처리구에서 낮게 검출되었다(p<0.05). Zearalenone은 대조구(600.33㎍/kg) 및 암모니아 처리구(376.00㎍/kg)에 비하여 가성소다 처리구(297.44㎍/kg)가 더 낮은 결과를 나타내었다(p<0.05). 볏짚 사일리지의 pH는 암모니아 처리구(4.63)가 가장 낮았고, 가성소다 처리구가 가장(8.19) 높았다(p<0.05). lactic acid 함량은 대조구와 암모니아 처리구(10.85와 10.98mM)는 유사한 수준이었으나 가성소다 처리구(8.80mM)가 가장 낮았다(p,0.05). Propionic acid는 대조구(0.55mM)가 화학처리구에 비하여 높았으며(p<0.05), 암모니아와 가성소다 처리구간(0.09와 0.07mM)에는 유사한 함량을 나타내었다. 화학처리에 따른 볏짚 사일리지의 반추위 미생물의 NDF 및 ADF의 분해율 모두 가성소다 처리구가 가장 높은 분해율을 보였고 다음으로 암모니아 처리구이었으며, 대조구가 가장 낮은 수준을 나타내었다(p<0.05). 따라서 본 연구결과는 볏짚 사일리지 제조공정에서 암모니아 및 가성 소다 처리가 곰팡이 독소 저감 및 볏짚 사일리지의 반추위 미생물 분해율 증진에 좋은 영향을 주는 것으로 사료되며, 가성소다 처리는 암모니아 처리에 비하여 곰팡이 독소 저감 및 섬유소 분해율 증진에 더 효율적이었으나 사일리지 발효에는 비효율적 영향을 준 것으로 사료된다.

Keywords

Mycotoxin; Ammonia; Sodium Hydroxide; Rice Straw silage;

Citations & Related Records

Reference

1	Huwig, A., Freimund, S., Kappeli, O. and Dutler, H. 2001. Mycotoxin detoxication of animal feed by different adsorbents. Toxicology Letters. 122:179-188. DOI
2	Jo, I.K., Hwangbo, S., Lee, Y.K., Ahn, J.H., Kim, H.J. and Lee, J.S. 2000. The effect of addition of apple pomace on quality and in situ degradability of rice straw silage. Journal of the Korean Society of Grassland Science. 20:295-302.
3	Cheli, F., Campagnoli, A. and Dell'Orto, V. 2013. Fungal populations and mycotoxins in silages: From occurrence to analysis. Animal Feed and Technology. 183:1-16. DOI
4	Kim, J.G., Ham, J.S., Chung, E.S., Yoon, S.H., Kim, M.J., Park, H.S., Lim, Y.C. and Seo, S. 2008. Evaluation of fermentation ability of microbes for whole crop rice silage inoculant. Journal of the Korean Society of Grassland Science. 28(3):229-236. DOI
5	Kim, S.R., Kim, G.S., Woo, J.H., Lee, J.W. and Sung, K.I. 2004. Chemical composition and fermentation characteristics of storage sections of the round bale silage of fresh rice straw at Yonchun of Gyeonggi-do. Journal of the Korean Society of Grassland Science. 24:253-260. DOI
6	Lee, S.C. 2000. Effects of chemical treatments and ensiling on the chemical composition and degradation rate in the rumen. Journal of the Korean Society of Grassland Science. 20:177-184.
7	Li, S., Marquardt, R.R., Frohlich, A.A., Vitti, T.G. and Crow, G. 1997. Pharmacokinetics of ochratoxin A and its metabolites in rats. Toxicology and Applied Pharmacology. 145:82-90. DOI
8	Mamun, M.A., Akbar, M.M.A. and Shahjalal, M. 2002. Rice straw, It's quality and quantity as affected by storage system in Bangladesh. Pakistan Journal of Nutrition. 1:153-155. DOI
9	Mashaly, E.D., Ismall, A.A. and Youssef, A. 1983. Effect of some chemical treatments on detoxification of aflatoxins in cotton seed meal. Proc. Int. Symp. Mycotoxins, Sept. 6-8th, 1981, Cairo, Egypt, the General Organization for Govt. pp. 515-522.
10	Mateo, J.J., Liorens, A., Mateo, R. and Jimenez, M. 2001. Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes. Journal of Chromatography. 918:99-112. DOI
11	Mehrez, A.A. and Orskov, E.I. 1977. A study of the artificial bac technique for determining the digestibility of feeds in the rumen. The Journal of Agricultural Science. 88:645-652. DOI
12	Moerch, K.E., McElfresh, P., Wohlman, A. and Hilton, B. 1980. Aflatoxin destruction in corn using sodium bisulfite, sodium hydroxide and aqueous ammonia. Journal of Food Protection. 43:571-574. DOI
13	Morgavi, D. and Riley, R.T. 2007. An historical overview of field disease outbreaks known or suspected to be caused by consumption of feeds contaminated with fusarium toxins. Animal Feed Science and Technology. 137:201-212. DOI
14	Nelson, C.E. 1993. Strategies of mold control in dairy feeds. Journal of Dairy Sciences. 76:898-902. DOI
15	Pestka, J.J. 2007. Deoxynivalenol: Toxicity, mechanisms and health risks. Animal Feed Science and Technology. 137:283-298. DOI
16	Sebunya, T.K. and Yourtee, D.M. 1990. Aflatoxigenic aspergilli in foods and feeds in Uganda. Journal of Food Quality. 13:97-101. DOI
17	Shin, K.J., Lee, B.D., Cha, Y.H., Lee, G.S. and Kim, K.S. 1989. Nutritive value of ammoniated rice straw in ruminants. I. Effect of various levels of ammonia treatment on consumption, digestibilities and nitrogen retention of rice straw in sheep. Korean Journal of Animal Sciences. 31:434-442.
18	Sung, H.G. 2014. Study on roughage degradation and adhesion of rumen fibrolytic bacteria by Real-Time PCR. Journal of the Korean Society of Grassland Science. 34:60-67. DOI
19	SPSS. 2017. Statistical package for the social sciences. IBM(R) SPSS(R) Statistics 25.0, IBM Now York USA.
20	Sung H.G. 2013. The studies on real condition of mycotoxin contamination in the fields before harvest and by the storage of rice straw using as roughage in Korea. Journal of The Korean Society of Grassland Science. 33:1-10. DOI
21	Sung, H.G., Lee, J.K. and Seo, S. 2011. Studies on fungi contamination and mycotoxins of rice straw round bale silage. Journal of the Korean Society of Grassland Science. 31:451-462. DOI
22	Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Carbohydrate methodology, metabolism, and nutritional implications in dairy cattle: Methods for dietary fibre, neutral detergent fibre, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74:3583-3597. DOI
23	Visconti, A. and Pascales, M. 1998. Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection. Journal of Chromatography A. 31:133-140. DOI
24	Voss, K.A., Smith, G.W. and Haschek, W.M. 2007. Fumonisins: Toxicokinetics, mechanism of action and toxicity. Animal Feed Science and Technology. 137:299-325. DOI
25	Wu, F. 2004. Mycotoxins risk assesment for the purpose of setting international regulatory standard. Environmental Science & Technology. 38:4049-4055. DOI
26	Alonso, V.A., Pereyra, C.M., Keller, L.A.M., Dalcero, A.M., Ros, C.A.R., Chiacchiera, S.M. and Cavaglieri, L.R. 2013. Fungi and mycrtoxin in silage: An overview. Journal of Applied Microbiology. 115:637-643. DOI
27	Wu, F. 2006. Economic impact of fumonisin and aflatoxin regulations on global corn and peanut markets. In: D. Barug, D. Bhatnager, H.P. Van Egmond, J.W. Van der Kamp, W.A. Van Osenbruggen and A. Viscinti (Eds.), The mycotoxin factbook. Food & Feed Topics. Wageningen Academic Publishers, The Netherlands.
28	Xiao, H., Marquardt, R.R., Abramson, D. and Frohlich, A.A. 1996. Metabolites of ochratoxins in rat urine and a culture of Aspergillus ochraceus. Applied and Environmental Microbiology. 62:648-655. DOI
29	Duncan, D.B. 1955. Multiple range and multiple F test. Biometrics. 11:1-42. DOI
30	Akiyama, H., Goda, Y., Tanaka, T. and Toyoda, M. 2001. Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up. Journal of Chromatography. 932:153-157. DOI
31	Atanda, S.A., Pessu, P.O., Aina, J.A., Agoda, S., Adekalu, O.A. and Ihionu, G.C. 2013. Mycotoxin management in agriculture. Greener Journal of Agricultural Sciences. 3:176-184.
32	Auerbach, H., Oldenburg E. and Weissbach F. 1998. Incidence of Penicillium roquiforti and Roquefortine C in silages. J. Sci Food Agric. 76:565-572. DOI
33	Fussel, R.J. and McCalley, D.V. 1987. Determination of volatile fatty acids(C2-C5) and lactic acid in silage by gas chromatography. Analyst. 112:1213-1216. DOI
34	Brekke, O.L., Sinnhuder, R.O., Peplinski, A.J., Wales, J.H., Putnam, G.B., Lee, D.J. and Ciegler, A. 1977. Aflatoxin in corn, ammonia inactivation and bioassay with rainbow trout. Applied and Environmental Microbiology. 34:34-37. DOI
35	Caloni, F., Spotti, M.. Auerbach, H., Op den camp, H., Fonk G.J. and Pompa, G. 2000. In vitro metabolism of fumonisin B1 by ruminal microflora. Veterinary Research Communications. 24:379-387. DOI
36	Fink-Gremmels, J. and Malekinejad, H. 2007. Clinical effects and biochemical mechanisms associated with exposure to the mycoestrogen zearalenone. Animal Feed Science and Technology. 137:326-341. DOI
37	Goering, H. and Van Soest, P.J. 1970. Forage Fiber Analysis. Agric. Handb. 379. US Department of Agriculture, Washington, DC.
38	Hammond, B.G., Campbell, K.W., Pilcher, C.D., Degooyer, T.A., Robinson, A.E., McMillen, B.I., Spangler, S.M., Riordan, S.G., Rice, L.G. and Richard, J.L. 2004. Lower fumonisin mycotoxin levels in the grain of Bt corn grown in the Unite State in 2000-2002. Journal of Agricultural and Food Chemistry. 52:1390-1397. DOI
39	Han, I.K. and Grrett, W.N. 1986. Improving the dry matter digestibility and voluntary intake of low quality by various treatment: A review. Korean Journal of Animal Sciences. 28:199-207.
40	Herrera-saldana, R., Church, D.C. and Kellem, R.D. 1982. The effect of ammonia treatment on intake and nutritive value of wheat straw. Journal of Animal Science. 54:603-608. DOI