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http://dx.doi.org/10.5333/KGFS.2011.31.3.277

Mycorrhizae Effects on N Uptake and Assimilation Estimated by 15N Tracing in White Clover under Water-Stressed Conditions  

Zhang, Qian (Chonnam National University)
Park, Sang-Hyun (Chonnam National University)
Kim, Tae-Hwan (Chonnam National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.31, no.3, 2011 , pp. 277-284 More about this Journal
Abstract
To investigate the effects of arbuscular mycorrhizal (AM) symbiosis on N uptake and its assimilation under drought-stressed conditions in white clover, total $^{15}N$ amount and $^{15}N$ amount incorporated into $NO_3^-$, amino acids and soluble proteins were quantified by $^{15}N$ tracing during 7 days of water treatment. Under well-watered conditions, there were no significant effects of AM symbiosis on all parameters analyzed in this study. Drought stress decreased total $^{15}N$ amount both in AM and non-AM plants, with a lower rate in AM plants (-13.8%) relative to non-AM plants (-28.5%) at day 7. Drought significantly increased $^{15}N-NO_3^-$ amount in non-AM plants. The amount of $^{15}N$-amino acids was 1.26-fold and 1.33-fold higher, respectively, in leaves and roots of AM plants compared to those of non-AM ones. Drought decreased the amount of $^{15}N$-soluble proteins in leaves at day 7, with a higher rate in non-AM plants than in AM ones. These results clearly indicate that AM colonization effectively alleviating the decrease in N uptake, amino acids and proteins synthesis caused by drought stress.
Keywords
Arbuscular mycorrhizae (AM); Drought; N uptake and assimilation; $^{15}N$ labeling;
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