Browse > Article
http://dx.doi.org/10.5333/KGFS.2009.29.4.291

Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Mature Seed Culture of Miscanthus sinensis  

Park, Choong-Hoon (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Yong-Goo (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Kyung-Hee (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Alam, Iftekhar (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Hyo-Jin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Sharmin, Shamima Akhtar (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Ki-Won (National Institute of Animal Science, RDA)
Lee, Byung-Hyun (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.29, no.4, 2009 , pp. 291-298 More about this Journal
Abstract
In order to optimize tissue culture conditions for genetic transformation of Miscanthus sinensis, we investigated the effects of different plant growth regulators on callus induction and plant regeneration using mature seeds as explant. Dehusked mature seeds were cultured on MS medium supplemented with 3 to 10 mg/L 2,4-D, dicamba or NAA, 30 g/L sucrose and 750 mg/L $MgCl_2{\cdot}6H_2O$. A number of combinations of auxin and cytokinin (BA, kinetin) were also used. MS medium containing 3 mg/L 2,4-D was found optimal for embryogenic callus induction (75.7%) from mature seed. The highest number of plants were regenerated (44.6%) upon transferring the embryogenic callus to MS medium supplemented with 1 mg/L 2,4-D plus 2 mg/L BA. This high efficient plant regeneration system could be useful to use for molecular breeding of new cultivars by genetic transformation.
Keywords
Callus; Plant growth regulators; Miscanthus;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Chaudhury, A. and Q. Rongda. 2000. Somatic embryogenesis and plant regeneration of turf-type Bermuda grass: effect of 6-benzyladenine in callus induction medium. Plant Cell Tiss. Org. Cult. 60:113-120   DOI   ScienceOn
2 Cho, M.J., W. Jiang and P.G. Laumaux. 1998. Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism. Plant Sci. 138:229-244   DOI   ScienceOn
3 Petersen, K.K., J. Hansen and P. Krogstrup. 1999. Significance of different carbon source and sterilization methods on callus induction and plant regeneration of Miscanthus x ogiformis Honda ‘Giganteus’. Plant Cell Tiss. Org. Cult. 58:189- 197   DOI   ScienceOn
4 Speller, C.S. 1993. The potential for growing biomass crops for fuel on surplus land in the UK. Outlook Agric. 22:23-29   DOI
5 Van, D., P. Valk, F. Ruis, A.M. Tettelaar-Schrier, D. Van and C.M. Velde. 1995. Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass, the effect of benzyladenine. Plant Cell Tiss. Org. cult. 40:101-103   DOI   ScienceOn
6 Zhong, H. and M.B. Sticklen. 1991. Plant regeneration via somatic embryogenesis in creeping bentgrass. Plant Cell Rep. 10:453-456   DOI
7 Holme, I.B. and K.K. Petersen. 1996. Callus induction and plant regeneration from different explant types of Miscanthus x ogiformis Honda ‘Giganteus’. Plant Cell Tiss. Org. Cult. 45:43-52   DOI   ScienceOn
8 Clifton-Brown, J.C., P.F. Stampfl and M.B. Jones. 2004. Miscanthus biomass production for energy in Europe and its potential contribution to deceasing fossil fuel carbon emissions. Global Change Biology. 10:509-519   DOI   ScienceOn
9 Clifton-Brown, J. C., Y.-C. Chiang and T. R. Hodkinson. 2008. Miscanthus: Genetic resources and breeding potential to enhance bioenergy production. pp. 273-294 in: Genetic Improvement of Bioenergy Crops, Vermerris, W.(Ed.). ISBN: 978-0-387-70804-1
10 Griffin, J.D. and M.S. Dibble. 1995. High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.). Plant Cell Rep. 14:721-724
11 Holme, I.B., P. Krogstrup and J. Hansen. 1997. Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus x ogiformis Honda ‘Giganteus’ as affected by proline. Plant Cell Tiss. Org. Cult. 50:203-210   DOI   ScienceOn
12 Jones, M.B. and M. Walsh. 2001. Miscanthus for energy and fibre. James and James (Science Publishers). London
13 우현숙, 이상훈, 이동기, 김진수, 원성혜, 이병현. 2004. 이탈리안 라이그래스의 성숙종자 유래 캘 러스로부터 효율적인 식물체 재분화. 한국식물생 명공학회지 31(1):43-48
14 Lewandowski, I., J.C. Clifton-Brown, J.M.O. Scurlock and W. Huisman. 2000. Miscanthus: European experience with a novel energy crop. Biomass and Bioenergy. 19:209-277   DOI   ScienceOn
15 Murashige, T. and F. Skoog. 1962. A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15:473-497   DOI
16 Petersen, K.K. 1997. Callus induction and plant regeneration in Miscanthus $\chi$ ogiformis Honda 'Giganteus' as influenced by benzyladenine. Plant Cell Tiss. Org. Cult. 49:137-140   DOI   ScienceOn
17 이상훈, 이동기, 김진수, 이병현. 2003. 오차드그 래스 성숙종자로부터 캘러스 유도 및 고효율 식물체 재분화. 한국식물생명공학회지 30(4):341-346
18 이상훈, 우현숙, 이병현. 2004a. 이탈리안 라이그 래스의 형질전환에 미치는 몇 가지 요인의 영향. 한국동물자원과학회지 46(2):235-242
19 이상훈, 김범수, 원성혜, 조진기, 김기용, 박근제, 성병렬, 이효신, 이병현. 2004b. 들잔디 성숙종자 로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향. 한국초지학회지 24(1): 29-36   DOI
20 임용우, 김기용, 최기준, 성병렬, 신정섭. 2000. 이탈리안 라이그라스 종자로부터 캘러스 유도 및 식물체 재분화. 한국초지학회지 20(1):25-30
21 Bai, Y. and R. Qu. 2000. An evaluation on callus induction and plant regeneration of turf-type tall fescue (Festuca arundinacea Schreb.) cultivars. Grass Forage Sci. 55:326-330   DOI   ScienceOn