Browse > Article
http://dx.doi.org/10.5333/KGFS.2009.29.2.095

Sulfur Deficiency Effects on Sulfate Uptake and Assimilatory Enzymes Activity in Rape Plants  

Li, Lu-Shen (Chonnam National University)
Jin, Yu-Lan (BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture & Life Science, Chonnam National University)
Lee, Bok-Rye (BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture & Life Science, Chonnam National University)
Kim, Tae-Hwan (Chonnam National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.29, no.2, 2009 , pp. 95-102 More about this Journal
Abstract
To determine $SO_4^{2-}$ uptake and its assimilation in response to the exogenous $SO_4^{2-}$supply level in forage rape (Brassica napus L.), the concentration of this element in plant tissues and the activity of ATP sulfurylasc and APS reductase was measured after 25 hours of treatment (1.0 mM $SO_4^{2-}$, control; 0.1 mM $SO_4^{2-}$, S deficiency; 0 mM $SO_4^{2-}$, S deprivation). $SO_4^{2-}$ uptake and the concentration in the plant tissues significantly decreased in S-deficient and S-deprived condition, while it maintained at nearly same level in the control. The activity of ATP sulfurylase tended to increase with decreasing the exogenous $SO_4^{2-}$ supply, while that of APS reductase to decrease. A significant change in both enzymes responding to S-deprivation treatment was observed only young and middle leaves. The results indicated that $SO_4^{2-}$ assimilation in young leaf tissues would be much more sensitively responded to S-limited nutrition.
Keywords
$SO_4^{2-}$; uptake; APS reductase; ATP sulfurylase; S deficiency; Rape;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zhao, F.J, P.E. Bilsborrow, E.J. Evans and S.P. McGrath. 1997. Nitrogen to sulfur ratio in rapeseed and in rapeseed protein and its use in diagnosing sulfur deficiency. J. Plant Nutr. 20:549-558   DOI   ScienceOn
2 Lappartient, A.G. and B. Touraine. 1996. Demanddriven complete solution of root ATP sulfurylase activity and $SO_4^2$- uptake in intact canola. Plant physiol. 111: 147-157
3 Leustek, T., M.N. Martin, J.A. Bick and J.P. Davies. 2000. Pathways and regulation of sulfur metabolism revealed through molecular and genetic studies. Annu. Rev. Plant Physiol. Plant Mol. BioI. 51:141-165   DOI   ScienceOn
4 Leustek, T. and K. Saito. 1997. Sulfate transport and assimilation in plants. Plant Physiol. 120: 637-643
5 Matsubayashi, Y., M. Ogawa, A. Morita and Y. Sakagami. 2002. An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine. Science. 296: 1470-1472   DOI   ScienceOn
6 Mechteld, M., A. Blake-Kalff, R. Kevin, M.J. Hawkesford, J.F. Zhao and S.P. McGrath. 1998. Distribution of sulfur within oilseed rape leaves in response to sulfur deficiency during vegetative growth. Plant Physiol. 118:1337-1344   DOI   ScienceOn
7 Schmidt, A and K. Jager. 1992. Open questions about sulfur metabolism in plants. Annu. Rev. Plant Physiol. Plant Mol. BioI. 43:325-349   DOI   ScienceOn
8 Schonhof, I., D. Blankenburg, S. muller and A. Krumbein. 2007. Sulfur and nitrogen supply influence growth, product appearance, and glucosinolate concentration of broccoli. J. Plant Nutr. Soli Sci. 170:65-72   DOI   ScienceOn
9 Sunarpi and J.W. Anderson. 1996. Distribution and redistribution of sulfur supplied as C5S] sulfate to roots during vegetative growth of soybean. Plant Physiol. 110:1151-1157   DOI
10 Suter, M., P. Ballmoos, S. Kopriva, R.O. Camp, J. Schaller, C. Kuhlemeier, P. Schurmann and C. Brunold. 2000. Adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes. J. BioI. Chem. 275:930-936   DOI   ScienceOn
11 Bloem, E., A. Riemenschneider, J. Volker, J. Papenbrock, A. Schmidt, L. Salac, S. Haneklaus and E. Schnug. 2004. Sulphur supply and infection with pyronopeziza brassicae influence Lcysteine desulphydrase acticity in Brassica napus L. J. Exp. Bot. 55:2305-2312   DOI   ScienceOn
12 Chew, F.S. 1988. Biological effects of glucosinolates. lnHG Cutler, ed, Biologically Active Natural Products: Potential Use in Agriculture. American Chemical Society, Washington, DC, pp: 155-181
13 Clarkson, D.T., F.W. Smith and P.J. Vanden Berg. 1983. Regulation of sulfate transport in a tropical Icgume, Macroptilium atropurpureum. J. Exp. Bot. 34:1463-1483   DOI
14 Gilbert, S.C., M. Plebanski, S.J. Harris, C.E.M. Allsopp, R Thomas, G.T. Layton and A.V.S. Hill. 1997. A protein particle vaccine containing multiple malaria epitopes. Nature biotech. 15:1280-1284   DOI   ScienceOn
15 Hell, R. 1997. Molecular physiology of plant sulfur metabolism. Planta 202:138-148   DOI   ScienceOn
16 Holmes, M.R.J. 1980. Nutrition of the Oilseed Rape Crop. Applied Science Publishers, London
17 Bell, C.J., D.T. Clarkson and W.J. Cram. 1995. Partitioning and redistribution of sulfur during Sstress in Macroptilium atropurpureum cv. Sirato. J. Exp. Bot. 46:73-81   DOI   ScienceOn
18 Hopkins, L., S. Parmar, D.L. Bouranis, J.R. Howarth and M.J. Hawkesford. 2004. Coordinated expression of sulfate uptake and components of the sulfate assimilatory pathway in maize. Plant BioI. 6:408-414   DOI   ScienceOn
19 Kim, T.H., A. Ourry, J. Boucaud and G. Lemaire. 1991. Changes in source sink relationship for nitrogen during regrowth of Lucerne (Medicago sativa L.) following removal of shoots. Aust. J. Plant Physiol. 18:593-602   DOI
20 Kopriva, S., T. Buchert, G. Fritz, M. Suter, R. Benda, V. Schunemann, A. Koprivova, P. Schurmann, A. X. Trautwein, P. M. H. Kroncek and C. Brunold. 2002. The presence of an ironsulfur cluster in adenosine 5'-phosphosulfate reductase separates organisms utilizing adenosine 5'-phosphosulfate and phosphoadenosine 5'- phosphosulfate for sulfate assimilation. J. BioI. Chem. 277:21786-21791   DOI   ScienceOn
21 Bick, J.A. and T. Leustek. 1998. Plant sulfur metabolism: the reduction of sulfate to sulfite. Curr. Opin. Plant BioI. 1:240-244   DOI   ScienceOn