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http://dx.doi.org/10.5333/KGFS.2009.29.2.103

Sulfur Deficiency Effects on Nitrate Uptake and Assimilatory Enzyme Activities in Rape Plants  

Li, Lu-Shen (Chonnam National University)
Jin, Yu-Lan (BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture & Life Science, Chonnam National University)
Lee, Bok-Rye (BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture & Life Science, Chonnam National University)
Kim, Tae-Hwan (Chonnam National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.29, no.2, 2009 , pp. 103-110 More about this Journal
Abstract
Rape plants, especially forage type, are known as one of high S-demanding plants. Their productivity and quality have often limited under S-deficient condition. To investigate the effect of S-deficiency on N uptake and its assimilation, $NO_3^-$ absorption, nitrate reducatse (NR) and glutamine synthetase (GS) activity in leaf and root tissues as affected by different S-supplied level was determined. $NO_3^-$ uptake was not significant between control and S-deficient treated plants, while significantly depressed in S-deprived plants for the early 8 h. NR activity decreased as S-availability decreased, especially in young and middle leaves, representing more than 35% of decrease in S-deficient and 70% in S-deprived plants when compared with control. In roots, a significant decrease (-29%) in NR was observed only in S-deprived plants. Relatively higher GS activity was found in young leaves for three all treatments. As a whole leaf tissue, S-limited conditions resulted in a reduction of GS activity. In root which showed the lowest activity, a significant decrease (-30%) was observed only in S-deprived plants.
Keywords
Glutamine synthetase; Nitrate reductase; N uptake; Rape; Sulfate level;
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