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http://dx.doi.org/10.7317/pk.2014.38.1.43

Synthesis and Characterization of Polyethylenimine-conjugated Polydiacetylene Liposome as a Gene Delivery Carrier  

Lee, Young Hwa (Department of Biochemistry, Chungnam National University)
Yim, Kang Hyuck (Department of Biochemistry, Chungnam National University)
Heo, Jungseok (Department of Chemistry, Chungnam National University)
Choi, Joon Sig (Department of Biochemistry, Chungnam National University)
Publication Information
Polymer(Korea) / v.38, no.1, 2014 , pp. 43-48 More about this Journal
Abstract
In this paper, we made a new polycationic polymeric liposome composed of low molecular weight polyethylenimine (PEI) and 10,12-pentacosadiynoic acid (PCDA). PCDA liposome was prepared by ultraviolet irradiation. PEI was further conjugated on the surface of the polymerized PCDA liposome using coupling reagents to make PCDA-PEI. The blue-to-red transition of PCDA liposome was observed during the coupling reaction. The size distribution of liposome and complexes with plasmid DNA was measured by dynamic light scattering (DLS). The complex formation was also identified by agarose gel electrophoresis and PicoGreen reagent assay. We confirmed the complex formation of the polymeric liposome with DNA and then performed transfection and cytotoxicity assay in HEK 293 and HeLa cells. As a result, PCDA-PEI showed significant gene transfection efficiency and low cytotoxicity. This study shows that PEI-conjugated PCDA liposome could be an efficient gene or drug delivery carrier.
Keywords
polydiacetylene; polyethylenimine; gene delivery; transfection;
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1 P. Hess, Clin. Lab. Med., 16, 197 (1996).
2 H. Eliyahu, Y. Barenholz, and A. J. Domb, Molecules, 10, 34 (2005).   DOI   ScienceOn
3 M. Thomas and A. M. Klibanov, Appl. Microbiol. Biotechnol., 62, 27 (2003).   DOI   ScienceOn
4 E. K. Ji, D. J. Ahn, and J. M. Kim, Bull. Korean Chem. Soc., 24, 667 (2003).   DOI   ScienceOn
5 E. Morin, M. Nothisen, A. Wagner, and J. S. Remy, Bioconjug. Chem., 22, 1916 (2011).   DOI   ScienceOn
6 R. R. Sawant, S. K. Sriraman, G. Navarro, S. Biswas, R. A. Dalvi, and V. P. Torchilin, Biomaterials, 33, 3942 (2012).   DOI   ScienceOn
7 D. H. Seo, B. C. Shin, and M. S. Kim, Polymer(Korea), 29, 277 (2005).
8 S. J. Bae, H. Choi, and J. S. Choi, Polymer(Korea), 37, 94 (2013).
9 Q. Cheng and R. C. Stevens, Langmuir, 14, 1974 (1998).   DOI   ScienceOn
10 A. Perino, A. Klymchenko, A. Morere, E. Contal, A. Rameau, J. Guenet, Y. Mely, and A. Wagner, Macromol. Chem. Phys., 212, 111 (2011).   DOI   ScienceOn