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http://dx.doi.org/10.7317/pk.2013.37.3.323

Effect of Inflammatory Responses to PLGA Films Incorporated Hesperidin: In vitro and In vivo Results  

Song, Jeong Eun (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Shim, Cho Rok (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Lee, Yujung (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Ko, Hyun Ah (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Yoon, Hyeon (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Lee, Dongwon (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Khang, Gilson (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
Publication Information
Polymer(Korea) / v.37, no.3, 2013 , pp. 323-331 More about this Journal
Abstract
Hesperidin (Hes) has known to having some functions like protection of blood circulatory system, anti-tumor effect, antioxidant effect and anti-inflammatory effect. The goal of this study is to demonstrate the relationship between Hes and inflammatory through in vitro and in vivo studies using poly(lactic-co-glycolic acid) (PLGA) film including Hes as a tissue engineered scaffold. To confirm the proliferation of cells on fabricated scaffold, cells (RAW 264.7 and NIH/3T3) were seeded on PLGA/Hes film then analyzed with MTT and SEM at 1 and 3 days after seeding. The results from ELISA, RT-PCR, and FACS for anti-oxident and anti-inflammatory effect showed that inflammatory response of PLGA/Hes film decreased more than that of PLGA film. Also, in vivo result confirmed that inflammatory response by implanted PLGA/Hes film decreased more comparing with PLGA film. This is because of anti-inflammatory effect of Hes reducing induced inflammatory cell and accumulation of fibrous capsule. The results showed that PLGA/Hes film's capacity on reducing inflammatory is better than PLGA film because of Hes.
Keywords
poly(lactic-co-glycolic acid); hesperidin; inflammatory response;
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