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http://dx.doi.org/10.7317/pk.2012.36.6.768

Effect of Ratio of Demineralized Bone Powder with Alginate Microcapsules on Articular Cartilage Regeneration  

Kim, A Ram (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Kim, Hye Min (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Lee, Jung Keun (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Lee, Ji Hye (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Song, Jeong Eun (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Yoon, Kun Ho (Division of Endocrinology and Metabolism, Deparyment of insternal Medicine, College of Medicine, The Catholic UNiversity of Korea)
Lee, Dongwon (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Khang, Gilson (Dept. of Bin Fusion Tech., Polymer Fusion Res. Center & Dept. of Polymer Nano Sci. Tech., Chonbuk National Univ.)
Publication Information
Polymer(Korea) / v.36, no.6, 2012 , pp. 768-775 More about this Journal
Abstract
Alginate, obtained from the seaweeds, is a widely used biomaterial for cell transplantation, since its positive effect on viability of capsulized cells and its easier encapsulation capability of living cells. Demineralized bone powder (DBP), derived from the natural bone tissue, is widely applied for clinical trials for its low rate of reaction and antigenicity. A chondrocyte was seeded into an alginate with DBP of different contents, and a microcapsule was produced. The adhesion and proliferation of cells was observed through the MTT analysis, and the PCR was applied to estimate the content of the glycosaminoglycan (sGAG) and collagen, and confirm the specific genetic pattern of the chondrocytes. Also, the alginate microcapsule where the chondrocyte is seeded was extracted after transplantation under the skin of a nude mouse, and was immunochemically stained. The experimental result confirmed that the alginate microcapsule containing 1% of DBP not only showed the highest proliferation of cell but had a positive effect of chondrocytes by the interaction between the alginates and the growth factor in DBP. It can be expected that the microcapsule with application of the alginates and DBP might be an appropriate scaffold for tissue engineering.
Keywords
alginate; demineralized bone powder; microcapsule; biocompatibility;
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