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Antibacterial Activity of Low Molecular Weight Water-Soluble Chitosan  

Park, Yoon-Kyung (Department of Biotechnology, Chosun University)
Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University)
Publication Information
Polymer(Korea) / v.35, no.5, 2011 , pp. 419-423 More about this Journal
Abstract
Chitosan is a natural polymer derived from chitin that has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. In addition, water-soluble chitosan has been used to enhance the stability of chitosan in water and reduce cytotoxic activity induced by acetic acid. In this study, the antibiotic activity and mechanism of low molecular weight water-soluble chitosan (LMWSC; MW1, MW3, MW5, and MW10) were examined in pathogenic bacteria cells and vesicles containing bacterial membrane lipids. MW10 displayed potent antibacterial activity against pathogenic bacteria strains and no cytotoxicity against mammalian cells. In addition, the degree of calcein leakage was examined as a function of lipid composition (PE/PG=7/3 w/w). The results of these experiments demonstrated that MW10 promoted leakage in negatively-charged membranes. Furthermore, confocal microscopy revealed that MW10 was located in the plasma membrane.
Keywords
chitosan; antibacterial activity; pathogenic bacteria; lipid; water-soluble; negatively-charged membranes;
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1 S. J. Howell, D. Wilk, S. P. Yadav, and C. L. Bevins, Peptides, 24, 1763 (2003).   DOI   ScienceOn
2 D. H. Schlesinger and G. Goldstein, Nature, 255, 423 (1975).   DOI   ScienceOn
3 N. R. Sudarshan, D. G. Hoover, and D. Knorr, Food Biotechnol., 6, 257 (1992).   DOI
4 K. Matsuzaki, K. Sugishita, and K. Miyajima, FEBS Lett., 449, 221 (1999).   DOI
5 Y. Pouny, D. Rapaport, A. Mor, P. Nicolas, and Y. Shai, Biochemistry, 31, 12416 (1992).   DOI   ScienceOn
6 M. Shimoda, K. Ohki, Y. Shimamoto, and O. Kohashi, Infect. Immun., 63, 2886 (1995).
7 S. B. Rao and C. P. Sharma, J. Biomed. Mater. Res., 34, 21 (1997).   DOI   ScienceOn
8 J. W. Nah and M. K. Jang, J. Polym. Sci. Part A: Polym. Chem., 40, 3796 (2002).   DOI   ScienceOn
9 Y. Park, M. H. Kim, S.-C. Park, H. Cheong, M.-K. Jang, J.-W. Nah, and K.-S. Hahm, J. Microbiol. Biotechnol., 18, 1729 (2008).
10 L. Illum, Pharm. Res., 15, 1326 (1998).   DOI   ScienceOn
11 G. Kisko, R. Sharp, and S. Roller, J. Appl. Microbiol., 98, 872 (2005).   DOI   ScienceOn
12 Y. C. Chung, Y. P. Su, C.C. Chen, G. Jia, H. L. Wang, J. C. G. Wu, and J. G. Lin, Acta Pharmacol. Sin., 27, 932 (2004).
13 I. M. Helander, E. L. Nurmiaho-Lassila, R. Ahvenainen, J. Rhoades, and S. Roller, Int. J. Food Microbiol., 71, 235 (2001).   DOI
14 P. J. Park, J. Y. Je, H. G. Byun, S. H. Moon, and S. K. Kim, J. Microbiol. Biotechnol., 14, 317 (2004).
15 P. J. Park, J. Y. Je, and S. K. Kim, Carbohydr. Polym., 55, 17 (2004).   DOI   ScienceOn
16 C. Porporatto, I. D. Bianco, C. M. Riera, and S. G. Correa, Biochem. Biophys. Res. Commun., 304, 266 (2003).   DOI   ScienceOn
17 Y. Maezaki, K. Tsuji, Y. Nakagawa, Y. Kawai, M. Akimoto, T. Tsugita, W. Takekawa, A. Terada, H. Hara, and T. Mitsuoka, Biosci. Biotechnol. Biochem., 57, 1439 (1993).   DOI
18 D. Raafat, K. von Bargen, A. Haas, and H. G. Sahl, Appl. Environ. Microbiol., 74, 3764 (2008).   DOI   ScienceOn
19 K. Suzuki, T. Mikami, Y. Okawa, A. Tokoro, S. Suzuki, and M. Suzuki, Carbohydr. Polym., 151, 403 (1986).
20 Y. J. Jeon and S. K. Kim, J. Chitin Chitosan, 6, 163 (2001).