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Effects of Attachment and Proliferation of Retinal Pigment Epithelial Cells on Silk/PLGA Film  

Jo, Eun-Hye (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Kim, Soo-Jin (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Cho, Su-Jin (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Lee, Ga-Young (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Kim, On-You (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Lee, Eun-Yong (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Cho, Won-Hyung (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Lee, Dong-Won (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Khang, Gil-Son (Department of BIN Fusion Tech, Dept. of Polymer.Nano Sci & Tech and Polymer Fusion Research Center, Chonbuk National University)
Publication Information
Polymer(Korea) / v.35, no.4, 2011 , pp. 289-295 More about this Journal
Abstract
Biomaterials for retinal tissue engineering must demonstrate several critical features for potential utility, including mechanical integrity, biocompatibility, and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. We prepared natural/synthetic hybrid silk/PLGA films using 0, 10, 20, 40, and 80 wt% of silk by a solvent evaporation method. MIT assay was used to confirm the number of cells attached on film at 1, 2, and 3 days, respectively. The morphology of cellular adhesion on films was also confirmed by scanning electron microscope (SEM). RT-PCR was conducted to confrrm mRNA expression of retinal pigment epithelitun (RPE) using RPE65 as a RPEs marker and the expression of cytokeratin were determined by immunofluorescence staining. We confirmed that the silk/PLGA film of 20~40 wt% silk was superior for the adhesion and proliferation of RPEs.
Keywords
silk; PLGA; silk/PLGA film; RPE;
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