Browse > Article

Preparation and Characterization of Sponge Using Demineralized Bone Particle  

Jang, Ji-Wook (Polymer Fusion Research Center, Chonbuk National University)
Baek, Mi-Ock (Polymer Fusion Research Center, Chonbuk National University)
Kim, Soon-Hee (Polymer Fusion Research Center, Chonbuk National University)
Choi, Jin-Hee (Polymer Fusion Research Center, Chonbuk National University)
Yang, Jae-Chan (Polymer Fusion Research Center, Chonbuk National University)
Hong, Hyun-Hye (Polymer Fusion Research Center, Chonbuk National University)
Hong, Hee-Kyung (Polymer Fusion Research Center, Chonbuk National University)
Rhee, John-M. (Polymer Fusion Research Center, Chonbuk National University)
Min, Byoung-Hyun (Cell Therapy Center, School of Medicine, Ajou University)
Khang, Gil-Son (Polymer Fusion Research Center, Chonbuk National University)
Publication Information
Polymer(Korea) / v.33, no.2, 2009 , pp. 104-110 More about this Journal
Abstract
Demineralized boneparticle (DBP) has been widely used as and a powerful promoter of new bone growth. In this study, DBP sponges were chemically crosslinked and characterized for the potential application of tissue engineered scaffolds. The DBP sponges prepared by crosslinking with EDC. 0.1, 0.2 or 0.3% pepsin was applied to DBP dissolved in 3% (v/v) acetic acid aqueous solution for 48 hrs. The prepared sponges were crosslinked by 1, 5, 10, 50 or 100 mM of EDC solution concentration and then were lyophilized. The DBP sponges were characterized by SEM, FT-IR and DSC and analyzed in terms of their porosity and water absorption ability. The cellular viability and proliferation were assayed by MTT assay. Our investigation revealed that 0.2$\sim$0.3% of pepsin and 50$\sim$100 mM of EDC produced DBP sponges with good physical characteristics. In conclusion, DBP sponge prepared under these conditions is potentially useful for the applications of tissue construction.
Keywords
demineralized bone particle; sponge; EDC; pepsin; BMSCs;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 W. Huang, C. Brian, W. Isabella, R. George, I. Jenji, W. Beniamin, Y. Dean, and M. Timothy, Experimental Cell Res., 299, 325 (2004)   DOI   ScienceOn
2 K. S. Kim, M. H. Cho, H. H. Ahn, S. B. Song, S. J. Seo, M. S. Kim, B. Lee, G. Khang, and H. B. Lee, Tissue Eng. Regen. Med., 4, 168 (2007)
3 S. K. Kim, K. D. Hong, S. H. Ko, M. S. Kim, G. Khang, I. W. Lee, C. W. Han, H. K. Lee, and H. B. Lee, Tissue Eng. Regen. Med., 2, 130 (2005)
4 J. R. Mauney, C. Jaquiery, V. Volloch, M. Hebererb, I. Martinb, and D. L. Kaplan, Biomaterials, 26, 3173 (2005)   DOI   ScienceOn
5 Y. Chang, C. C. Tsail, H. C. Lian, and H. W. Sung, Biomaterials, 23, 2447 (2002)   DOI   ScienceOn
6 V. Charulatha and A. Rajaram, Biomaterials, 24, 759 (2003)   DOI   ScienceOn
7 Y. Zhang, W. Liu, G. Li, B. Shi, Y. Miao, and X. Wub, Food Chem., 103, 906 (2007)   DOI   ScienceOn
8 J. W. Jang, B. Lee, C. W. Han, M. S. Kim, S. H. Cho, H. B. Lee, and G. Khang, Polymer(Korea), 28, 382 (2004)   과학기술학회마을
9 Y. K. Ko, S. H. Kim, J. S. Jeong, J. S. Park, J. Y. Lim, M. S. Kim, H. B. Lee, and G. Khang, Polymer(Korea), 31, 505 (2007)   과학기술학회마을
10 E. J. Kim, C. S. Park, C. S. Park, M. S. Kim, S. H. Cho, J. M. Rhee, H. B. Lee, and G. Khang, Polymer(Korea), 28, 218 (2004)   과학기술학회마을
11 G. Khang, S. J. Lee, and H. B. Lee, 'Polymer-cell interaction', in Tissue Engineering: Concepts and Application, 2nd Eds., J. J. Yoon, and I. Lee, Editors, Korea Med. Pub. Co., Seoul, p. 297 (2002)
12 M. O. Baek, S. J. Won, S. H. Kim, H. W. Roh, N. R. Lee, M. S. Kim, G. H. Ryu, Y. H. Cho, S. J. Lee, G. Khang, and H. B. Lee, Tissue Eng. Regen. Med., 4, 600 (2007)   DOI   ScienceOn
13 M. R. Urist, Science, 150, 893 (1965)   DOI   PUBMED   ScienceOn
14 S. H. Kim, S. H. Kim, and Y. H. Kim, Polym. Sci. Tech., 16, 468 (2005)
15 M. Matsuoka, B. Wispriyono, and H. Igisu, Biochem. Pharm., 59, 1573 (2000)   DOI   ScienceOn
16 Y. K. Ko, S. H. Hee, H. J. Ha, M. S. Kim, C. W. Han, J. M. Rhee, Y. S. Son, H. B. Lee, and G. Kang, Tissue Eng. Regen. Med., 4, 67 (2007)
17 I. K. Park, S. H. Oh, and J. H. Lee, Tissue Eng. Regen. Med., 1, 164 (2004)
18 S. Nalinanon, S. Benjakul, W. Visessanguan, and H. Kishimura, Food Chem., 104, 593 (2007)   DOI   ScienceOn
19 Z. Chen, X. Mo, C. He, and H. Wang, Carbohydrate Polym., 72, 410 (2008)   DOI   ScienceOn
20 H. W. Shin, S. H. Kim, J. W. Jang, M. S. Kim, S. H. Cho, H. B. Lee, and G. Khang, Polymer(Korea), 28, 194 (2004)   과학기술학회마을
21 S. H. Kim, S. J. Yoon, J. W. Jang, M. S. Kim, G. Khang, and H. B. Lee, Polymer(Korea), 30, 14 (2006)   과학기술학회마을
22 S. J. Yoon, K. S. Park, B. S. Choi, G. Khang, M. S. Kim, J. M. Lee, and H. B. Lee, Key Eng. Materials, 342-343, 161 (2007)   DOI
23 G. Khang, M. S. Kim, B. Y. Min, I. W. Lee, J. M. Rhee, and H. B. Lee, Tissue Eng. Regen. Med., 3, 376 (2006)
24 G. Khang, M. S. Kim, S. H. Cho, I. W. Lee, J. M. Rhee, and H. B. Lee, Tissue Eng. Regen. Med., 1, 9 (2004)
25 J. F. Sugiura, H. Kitoh, and N. Ishiguro, BBRC, 316, 233 (2004)   DOI   ScienceOn
26 S. N. Park, H. J. Lee, K. H. Lee, and H. Suh, Biomaterials, 24, 1631 (2003)   DOI   ScienceOn
27 B. S. Choi, S. H. Kim, S. J. Yun, H. H. Ha, M. S. Kim, Y. I. Yang, Y. S. Son, G. Khang, J. M. Rhee, and H. B. Lee, Tissue Eng. Regen. Med., 3, 295 (2006)