Browse > Article

Adhesion of Human Intervertebral Disk Cells on Aiginate/PLGA Microspheres  

Lee, Jun-Hee (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
Jang, Ji-Wook (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
So, Jeong-Won (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
Choi, Jin-Hee (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
Park, Jong-Hak (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
Ahn, Shik-Il (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
Son, Young-Suk (College of Life Science, Kyung Hee University)
Min, Byoung-Hyun (Cell Therapy Center, School of Medicine, Ajou University)
Khang, Gil-Son (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University)
Publication Information
Polymer(Korea) / v.33, no.1, 2009 , pp. 7-12 More about this Journal
Abstract
PLGA microspheres have been known as an injectable system for tissue engineering. The purpose of this study was to investigate the condition of emulsion formation and cell adhesion on the microsphere surface. BSA-loaded PLGA microsphere was fabricated by oil-in-water (O/W) and water-in-oil-in-water (W/O/W) solvent evaporation method. Sodium alginate was dissolved in water phase to control initial burst release and to improve lag time by PLGA bulk degradation. In addition, the morphology of cells attached on the micro spheres was studied using a scanning electron microscopy (SEM). Cellular proliferation behavior of human disc cells cultivated on PLGA micro spheres was analyzed using a MTT assay. MTT assay revealed that the cells can attach and proliferate on PLGA microspheres. According to these results, we concluded that BSA -loaded alginate/PLGA microspheres can be used as an injectable system for tissue engineering application.
Keywords
alginate; PLGA; microsphere; cell adhesion; initial burst;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 E. Piskin, J. Biomat. Sci. Polym. Ed., 6, 775 (1995)   DOI   ScienceOn
2 H. J. Chung and T. G. Park, Adv. Drug. Deliver. Rev., 59, 249 (2007)   DOI   ScienceOn
3 B. Bondar, S. Fuchs, A. Motta, C. Migliaresi, and C. J. Kirkpatrick, Biomaterials, 29, 561 (2008)   DOI   ScienceOn
4 J. P. Benoit, N. Faisant, M. C. Venier-Juliene, and P. Menei, J. Control. Release, 65, 285 (2000)   DOI   ScienceOn
5 S. Cohen, T. Yoshioka, M. Laucarelli, L. H. Hwang, and R. Langer, Pharm. Res., 8, 713 (1991)   DOI   ScienceOn
6 H. Marchais, F. Boury, C. Damge, J. E. Proust, and J. P. Benoit, S.T.P. Pharma. Sci., 6, 417 (1996)
7 S. L. Lyons and S. G. Wright, US Patent 6,331,317, (2001)
8 K. G. Carrasquillo, A. M. Stanley, J. C. Aponte-carro, P. D. Jesus, H. R. Costantino, C. J. Bosques, and K. Grebenow, J. Control. Release, 76, 199 (2001)   DOI   ScienceOn
9 J. I. Lee and H. S. Yoo, Colloids Surf. B Biointerfaces, 61, 81 (2008)   DOI   ScienceOn
10 M. O'Mahony, Sensory Evaluation of Food: Statistical Methods and Procedures, CRC Press, p. 487 (1986)
11 G. Khang and H. B. Lee, Biomedical Polymers, p.19 (2001)
12 H. William, S. A. Teukolsky, W. T. Vetterling, and B. P. Flannery, Numerical Recipes in C: The Art of Scientific Computing, Cambridge University Press, p. 616 (1992)
13 H. Jeffery, S. S. Davis, and D. T. O'Hagan, Pharm. Res., 10, 362 (1993)   DOI   ScienceOn
14 P. Sansdrap and A. J. Moes, Int. J. Pharm., 98, 157 (1993)   DOI   ScienceOn
15 A. Goraltchouk, V. Scanga, C. M. Morshead, and M. S. Shoichet, J. Control. Release, 110, 400 (2006)   DOI   ScienceOn
16 M. J. Mahoney and W. M. Saltzman, J. Pharm. Sci., 90, 1356 (1996)   DOI   ScienceOn
17 S. M. Kim, S. H. Kim, C. M. Kim, A. Y. Oh, G. A. Kim, I. W. Lee, J. M. Rhee, G. Khang, M. S. Kim, and H. B. Lee, Tissue Eng. Regen. Med., 4, 589 (2007)
18 M. K. Choi, G. Khang, I. W. Lee, J. M. Rhee, and H. B. Lee, Polymer(Korea), 25, 318 (2001)
19 L. S. Nair and C. T. Laurencin, Pro. Polym. Sci., 32, 762 (2007)   DOI   ScienceOn
20 P. Couvreur, M. J. Blanco-prieto, F. Puisieux, B. Roques, and E. Fattal, Adv. Drug. Deliver. Rev., 28, 85 (1997)   DOI   ScienceOn
21 J. Herrmann and R. Bodmeier, Int. J. Pharm., 126, 129 (1995)   DOI   ScienceOn
22 G. Khang, J. H. Jeon, J. C Cho, and H. B. Lee, Polymer (Korea), 23, 471 (1999)
23 N. Nihant, C. Schugens, C. Grandfils, R. Jerome, and P. Teyssile, Pharm. Res., 11, 1479 (1994)   DOI   ScienceOn
24 R. Jeyanthi, R. C. Metha, B. C. Thanoo, and P. P. Deluca, J. Microencapsul., 14, 163 (1997)   DOI   ScienceOn
25 S. K. Kim, S. H. Kim, H. R. Lee, M. H. Cho, M. S. Kim, G. Khang, and H. B. Lee, Tissue Eng. Regen. Med., 2, 388 (2005)
26 H. Y. Lee, H. S. Lee, J. S. Kim, S. B. Kim, J. S. Lee, H. I. Choi, and S. G. Chang, International Patent WO 01/78687 A1 (2001)
27 E. K. Jeon, H. J. Whang, G. Khang, I. Lee, J. M. Rhee, and H. B. Lee, Polymer(Korea), 25, 893 (2001)   과학기술학회마을
28 S. J. Lee, S. H. Oh, J. Liu, S. Soker, A. Atala, and J. J. Yoo, Biomaterials, 29, 1442 (2008)
29 L. Meinel, O. E. Illi, J. Zapf, M. Malfanti, H. P. Merkle, and B. Gander, J. Control. Release, 70, 193 (2001)   DOI   ScienceOn
30 J. W. So, S. H. Kim, M. O. Baek, Y. Y. Lim, H. W. Roh, N. R. Lee, M. S. Kim, G. H. Ryu, Y, Cho, S. J. Lee, B. H. Min, G. Khang, and H. B. Lee, Tissue Eng. Regen. Med., 4, 577 (2007)   DOI   ScienceOn
31 Y. Y. Yang, T. S. Chung, X. L. Bai, and W. K. Chan, Chem, Eng, Sci., 55, 2223 (2002)   DOI   ScienceOn
32 S. Y. Chew, R. Mi, A. Hoke, and K. W. Leong, Biomaterials, 29, 653 (2008)   DOI   ScienceOn
33 J. S. Park, J. C. Yang, S. H. Yuk, H. S. Shin, J. M. Rhee, M. S. Kim, H. B. Lee, and G. Khang, Polymer(Korea), 31, 189 (2007)   과학기술학회마을
34 Y. K. Ko, S. H. Kim, J. S. Jeong, H. J. Ha, S. J. Yoon, J. M. Rhee, M. S. Kim, H. B. Lee, and G. Khang, Polymer(Korea), 31, 14 (2007)   과학기술학회마을
35 T. G. Park, H. Y. Lee, and Y. S. Nam, J. Control. Release, 55, 181 (1998)   DOI   ScienceOn
36 S. H. Choi and T. G. Park, Int. J. Pharm., 203, 193 (2000)   DOI   PUBMED   ScienceOn
37 S. M. Willerth and S. E. Sakiyama-Elbert, Adv. Drug. Deliver. Rev., 59, 325 (2007)   DOI   ScienceOn
38 F. Gabor, B. Ertl, M. Wirth, and R. Mallinger, J. Microencapsul., 16, 1 (1999)   DOI   PUBMED   ScienceOn
39 H. Sah, R. Toddywala, and Y. W. Chien, J. Control. Release, 30, 201 (1994)   DOI   ScienceOn
40 S. M. Kim, S. H. Kim, C. M. Kim, A. Y. Oh, I. W. Lee, M. S. Kim, J. M. Rhee, G. Khang, and H. B. Lee, Tissue Eng. Regen. Med., 4, 583 (2007)
41 C. Schugens, N. Laruelle, N. Nihant, C. Grandfils, R. Jerome, and P. Teyssie, J. Control. Release, 32, 161 (1994)   DOI   ScienceOn