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Evaluation of Various Scaffolds for Tissue Engineered Biodisc Using Annulus Fibrosus Cells  

Ha, Hyun-Jung (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Soon-Hee (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Yoon, Sun-Jung (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Park, Sang-Wook (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
So, Jung-Won (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Moon-Suk (Nanobiomaterials Lab, Korea Research Institute of Chemical Technology)
Rhee, John-M. (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Khang, Gil-Son (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Lee, Hai-Bang (Nanobiomaterials Lab, Korea Research Institute of Chemical Technology)
Publication Information
Polymer(Korea) / v.32, no.1, 2008 , pp. 26-30 More about this Journal
Abstract
This study was designed to investigate the effect of hybridization of synthetic/natural materials for annulus fibrosus (AF) tissue regeneration in vitro and in vivo. The synthetic/natural hybrid scaffolds were prepared using PLGA (poly (lactic-co-glycolic) acid), SIS (small intestinal submucosa) and DBP (demineralized bone particles). PLGA, PLGA/SIS(20%), PLGA/DBP(20%) and PLGA/SIS (10%)/DBP (10%) scaffold were manufactured by solvent casting/salt leaching method. Compressive strength was measured. Rabbit AF cells were isolated, cultured and seeded into experimental groups. Hydroxyproline production and DNA quantity of AP cells on each scaffold was measured at 2, 4 and 6 weeks after in vitro culture. Cell-scaffold composites were implanted subcutaneously into athymic mice. After 1,4 and 6 weeks postoperatively, specimens were taken and H&E, Safranin-O and type I collagen staining were carried out concerning formation of cartilagenous tissue. In vitro PLGA/SIS scaffold was evaluated for total collagen content (bydroryproline/DNA content) and PLGA scaffold was evaluated for compressive strength.
Keywords
intervertebral disc regeneration; tissue engineering; natural-synthetic hybrid materials; annulus fibrosus cell; small intestinal submucosa;
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