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Preparation and Characterization of Microparticles of $Poly(\gamma-glutamic\;acid)$ Containing Lysozyme by means of Supercritical Anti-Solvent (SAS) Precipitation Process  

Lee, Dong-Il (Department of Polymer Science and Engineering, Kumoh National Institute of Technology)
Ling, Yun (Department of Polymer Science and Engineering, Kumoh National Institute of Technology)
Sung, Moon-Hee (Department of Chemistry, Kookmin University)
Park, Il-Hyun (Department of Polymer Science and Engineering, Kumoh National Institute of Technology)
Publication Information
Polymer(Korea) / v.31, no.2, 2007 , pp. 168-176 More about this Journal
Abstract
The sub-micron particles of poly ($\gamma-glutamic$ acid) (PGA) containing lysozyme have been prepared using supercritical antisolvent (SAS) precipitation process at various experimental conditions such as pressure, temperature, inner diameter of nozzle, and concentration. As overall results of the application of SAS process to this system, the smaller size powder has been produced as (i) increasing pressure, (ii) decreasing temperature, (iii) decreasing the inner diameter of nozzle, and (iv) decreasing the concentration of PGA and lysozyme. It is found by means of FT-IR analysis that during SAS process, the composition has changed from the original composition of PGA : lysozyme=50 : 50 into PGA : lysozyme=33 : 67 at final product powder. It means that PGA has higher solubility for the mixed solvent of carbon dioxide and dimethyl sulfoxide (DMSO). Due to such difference of solubility, this particle forms the core-shell structure of which the core consists mainly of lysozyme. It is also found that the residual DMSO amount of $7.8\times10^{-3}wt%$ exists inside the powder.
Keywords
supercritical anti-solvent; poly($\gamma-glutamic$ acid); lysozyme; microparticle; core-shell structure;
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