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Preparation of Cationic Liposomes Modified by Polyethylenimine and Their Application as Gene Carrier  

Seo, Dong-Hoan (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Shin, Byung-Cheol (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Kim, Moon-Suk (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Publication Information
Polymer(Korea) / v.29, no.3, 2005 , pp. 277-281 More about this Journal
Abstract
Recently, various curriers prepared by the modification both cationic polymers and liposomes have been examined. In this work, we prepared the lipid with polyethylenimine (PEI) to investigate the possibility as effective DNA carrier. Cationic lipid (PEI-DSPE) was synthesized by the reaction of PEI and 1,2-diacyl-sn-glycero-3-phosphoetha-nolamine (DSPE). The liposomes were prepared by the concenoation changes of PEI-DSPE for a mixture of 1,2-disteanyl-sn-glycero-3-phosphocholine (DSPC), L-$\alpha$-phosphatidylcholine, hydrogenated (HSPC) and cholesterol (CHOL). Particle size decreased as PEI-DSPE concentration increased. In addition, the charge of liposome surface increased to positive value according to increasing the relative of PEI-DSPE concentration. The complexation of DNA was confirmed by gel retardation assay and fluorescence measurement. The surface charge of liposome/DNA complexes increased as the liposome concentration or surface charge of liposome increased. In conclusion, we confirmed that the prepared liposomes have the possibility as a DNA carrier.
Keywords
PEI-DSPE; liposome; gene carrier;
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