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Preparation and In Vitro Release of DNA-Loaded Poly(D,L-lactic-co-glycolic acid) Microspheres  

Son, Hye-Jung (College of Pharmacy, Sookmyung Women's University)
Kim, Jin-Seok (College of Pharmacy, Sookmyung Women's University)
Publication Information
Polymer(Korea) / v.29, no.1, 2005 , pp. 69-73 More about this Journal
Abstract
To overcome the main disadvantages of non-viral gene delivery systems such as repeated administration due to the low transfection efficiency, poly(D,L-lactide-co-glycolide) was applied to encapsulate pDNA in its microsphere formulation. Free pDNA or various ratios (w/w) of chitosan/pDNA complexes was used for encapsulation, with the resulting encapsulation efficiency of 44%, 5%, and 8% for free pDNA, 0.7:1 and 1:1 ratios, respectively. Scanning electron micrographs of poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres encapsulating pDNA or chitosan-condensed pDNA revealed a smooth spherical shape immediately after microsphere preparation and a collapsed porous shape in 41 days due to the degradation of PLGA. In vitro release profile showed that the 0.7:1 (w/w) ratio formulation exerted 47% release in 26 days, whereas free pDNA or 1:1 (w/w) ratio formulation did only 15% or 32%, respectively.
Keywords
microsphere; PLGA; DNA; chitosan;
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1 K. W. Leong, H. Q. Mao, V. L. Truong-Le, K. Roy, S. M. Walsh, and J. T. August, J. Control. Rel., 53, 183 (1998)
2 G. Khang, S. A. Seo, H. S. Choi, J. M. Rhee, and H. B. Lee, Macromol. Res., 10, 246 (2002)
3 J. W. Yockman, A. Maheshwari, S. Han, and S. W. Kim, J. Control. Rel., 87, 177 (2003)   DOI   ScienceOn
4 M. J. Brunda, J. Leukocyte BioI., 55, 280 (1994)
5 D. J. Verbik, W. W. Stinson, M. J. Brunda, A. Kessinger, and S. S. Joshi, Clin. Exp. Metastasis, 14, 219 (1996)
6 T. Hao, U. McKeever, and M. L. Hedley, J. Control. ReI., 69, 249 (2000)
7 M. Nakanishim, Crit. Rev. Ther. Drug Carr. Syst., 12, 263 (1995)
8 T. Tada, S. Ohzeki, K. Utsumi, H. Takiuchi, M. Muramatsu, X. F. Li, J. Shimizu, H. Fujiwara, and T. Hamaoka, J. lmmunol., 146, 1077 (1991)
9 H. Temin, Hum. Gene Ther., 1, 111 (1990)
10 F. C. MacLaughlin, R. J. Mumper, J. Wang, J. M. Tagliaferri, I. Gill, M. Hinchcliffe, and A. P. Rolland, J. Control. ReI., 56, 259 (1998)
11 S. K. Liao, C. C. Hung, and M. F. Lin, Macromol. Res., 12, 466 (2004)
12 A. Aiuti, F. Ficara, F. Cattaneo, C. Bordignon, and M. G. Roncarolo, Curr. Opin. Allergy. Clin. Immunol., 3, 461 (2003)
13 P. Felgner, Adv. Drug Deliv. Rev., 5, 807 (1990)
14 A. Kabanov and V. Kabanov, Bioconjug. Chem., 6, 7 (1995)
15 T. Merdan, J. Kopecek, and T. Kissel, Adv. Drug. Deliv. Rev., 54, 715 (2002)
16 B. Sangro, G. Mazzolini, J. Ruiz, M. Herraiz, J. Quiroga, I. Herrero, A. Benito, J. Larrache, J. Pueyo, J. C. Subtil, C. Olague, J. Sola, B. Sadaba, C. Lacasa, I. Melero, C. Qian, and J. Prieto, J. Clin Oncol., 22, 1389 (2004)   DOI   ScienceOn
17 M. J. Brunda, L. Luistro, P. R. Warrier, R. B. Wright, B. R. Hubbard, M. Murphy, and S. F. Wolf, J. Exp. Med., 178, 1223 (1993)
18 Y. Noguchi, E. C. Richards, Y. T. Chen, and L. J. Old, Proc. Natl. Acad. Sci., 92, 2219 (1995)
19 Y. Kato, H. Onishi, and Y. Machida, Macromol. Res., 11, 382 (2003)
20 S. P. Bannan, L. Lunsford, P. Chambers, and M. L. Hedley, J. Control. ReI., 69, 337 (2000)
21 K. Y. Lee, I. C. Kwon, Y. H. Kim, W. H. Jo, and S. Y. Jeong, J. Control. ReI., 51, 213 (1998)
22 V. L. Singer, L. J. Jones, S. T. Yue, and R. P. Haugland, Anal. Biochem., 249, 228 (1997)
23 G. Khang, J. M. Rhee, J. K. Jeong, J. S. Lee, M. S. Kim, S. H. Cho, and H. B. Lee, Macromol. Res., 11, 207 (2003)
24 W. Zauner, N. A. Farrow, and A. M. R. Haines, J. Control. Rel., 71, 39 (2001)   DOI   ScienceOn
25 R. I. Mahato, M. Lee, S. Han, A. Maheshwari, and S. W. Kim, Mol. Ther., 4, 130 (2001)   DOI   ScienceOn
26 L. B. Couto, Semin. Thromb. Hemost., 30, 161 (2004)