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Preparation of Insulin-Immobilized Polyurethane Films and Their Interaction with Fibroblasts  

맹만 (연변대학교 화학공학과(중국))
김우식 (경북대학교 고분자공학과)
이동호 (경북대학교 고분자공학과)
박이순 (경북대학교 고분자공학과)
강인규 (경북대학교 고분자공학과)
Publication Information
Polymer(Korea) / v.28, no.6, 2004 , pp. 538-544 More about this Journal
Abstract
Polyurethanes containing L-lysine segments in the main chain (PULL) were synthesized from 4,4'-diphenymethyl diisocynate, poly(tetramethylene glycol), and z-lysine oligomer as a chain extender. Insulin-immobilized polyurethanes (PULL-In) were prepared by a coupling reaction of PULL surface amino groups with insulins. The amount of immobilized insulin was about 0.30 nmol/$\textrm{cm}^2$, as determined by Bradford method. The interactions of NIH/3T3 fibroblasts with surface-modified PULLs were investigated using $^3$H-thymidine incoporation and optical microscopy. The cell growth rate on PULL-In film was higher than those on other substrates. The cell proliferation by the immobilized insulin was almost same as that by the free one.
Keywords
insulin; polyurethane; fibroblasts; surface modification; proliferation;
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